Top Document: Sci.chem FAQ - Part 6 of 7 Previous Document: 30. Polymer Chemistry See reader questions & answers on this topic! - Help others by sharing your knowledge 31.1 How does remote sensing of chemical pollutants work?. The are several techniques, but the one of most interest to the public is the system being used to identify grossly polluting vehicles. The system consists of an infra-red source on one side of the road, and a detector system on the other. The collimated beam of IR is directed at a gas filter radiometer equipped with two liquid-nitrogen-cooled indium antimide photovoltaic detectors. The beam is split, and passes through a 4.3um bandpass filter to isolate the CO2 spectral region, a 4.6um filter to isolates the CO region, and a third filter to isolate the HC region. A non-absorbing region is also used to compensate for signal strength. There are various specific enhancements, such as the spinning gas-filter correlation cell in the University of Denver FEAT ( Fuel Efficiency Automobile Test ) system used to cost-effectively identify grossly-polluting vehicles [1]. "Optical remote sensing for air pollutants - review " by M.Simonds et al [2], provides a good introduction to the diverse range of instruments used for remote sensing of pollutants. 31.2 How does a Lava Lamp work?. Contributed by: Jim Webb <jnw4347@email.unc.edu> A container filled with clear or dyed liquid contains a non-water-soluble substance (the "lava") that's just a little bit denser (heavier), and has a greater thermal coefficient of expansion, than the liquid around it. Thus, it settles to the bottom of the container. A heat source at the bottom of the container warms the substance, making it expand and become less dense than the liquid around it. Thus, it rises. As it moves away from the heat source, it cools, contracts a bit, and becomes (once again) heavier than the medium. Thus, it falls. Heavy, light, heavy, light. Sounds like a Milan Kundera novel. (Actually, to be more precise: dense, less dense, dense, less dense.) 31.3 How do I make a Lava Lamp?. Contributed by: Jim Webb <jnw4347@email.unc.edu> Method 1. A new, easy, simple, cheap lava lamp recipe Use mineral oil as the lava. Use 90% isopropyl alcohol (which most drugstores can easily order) and 70% isopropyl alcohol (grocery-store rubbing alcohol) for the other ingredient. In 90% alcohol the mineral oil will sink to the bottom; slowly add the 70% alcohol (gently mixing all the while; take your time) until the oil seems lighter and is about to "jump" off the bottom. Use the two alcohols to adjust the responsiveness of the "lava." This mixture is placed in a closed container (the "lava lamp shape" is not required, although something fairly tall is good) and situated over a 40-watt bulb. If the "lava" tends to collect at the top, try putting a dimmer on the bulb, or a fan at the top of the container. To dye the lava, use an oil-based dye like artists' oil paints or a chopped-up sharpie marker. To dye the liquid around it, use food coloring. Two suggestions for better performance: 1) Agitation will tend to make the mineral oil form small bubbles unlike the large blobs we're all used to. The addition of a hydrophobic solvent to the mixture will help the lava coalesce. Turpentine and other paint solvents work well. To make sure what you use is hydrophobic, put some on your hand (if it's so toxic you can't put it on your hand, do you want to put it in a container that could break all over your room/desk/office?) and run a little water on it. If the water beads, it should work fine. 2) For faster warm-up time, add some antifreeze or (I've not tried it) liquid soap. Too much will cloud the alcohol. Keep in mind that the addition of these chemicals may necessitate your readjusting the 90% to 70% alcohol mixture. Method 2. The "official" way - from a patent [3]. The patent itself is not very specific as to proportions of ingredients. The solid component (i.e., the waxy-looking stuff that bubbles) is said to consist of "a mineral oil such as Ondina 17 (R.T.M.) with a light paraffin, carbon tetrachloride, a dye and paraffin wax." The medium this waxy stuff moves in is roughly 70/30% (by volume) water and a liquid which will raise the coefficient of cubic thermal expansion, and generally make the whole thing work better. The patent recommends propylene glycol for this; however, glycerol, ethylene glycol, and polyethylene glycol (aka PEG) are also mentioned as being sufficient. This mixture is placed in a closed container (the "lava lamp shape" is not required, although something fairly tall is good) and situated over a 40-watt bulb. If the "lava" tends to collect at the top, try putting a dimmer on the bulb, or a fan at the top of the container. Method 3. The "less official" way - from Popular Electronics [4]. Several non-water-soluble chemicals fall under the category of being "just a little bit heavier" than water, and are still viscous enough to form bubbles, not be terribly poisonous, and have a great enough coefficient of expansion. Among them: Benzyl alcohol (Specific Gravity 1.043 g/cm3), Cinnamyl Alcohol (SG 1.04), Diethyl phthalate (SG 1.121) and Ethyl Salicylate (SG 1.13). [The specific gravity of distilled water is 1.000.] Hubscher recommends using Benzyl Alcohol, which is used in the manufacture of perfume and (in one of its forms) as a food additive. It can be obtained from chemical or laboratory supply houses (check your yellow pages); the cheapest I could find it for was $25 for 500 ml (probably 2, maybe 3 regular-sized lava lamps' worth). An oil-soluble dye is nice to color the "lava"; Hubscher soaked the benzyl in a chopped up red felt-tip pen and said it worked great. [Benzyl alcohol is "relatively harmless", but don't drink it, and avoid touching & breathing it.] Hubscher found that the benzyl and the water alone didn't do much, so he raised the specific gravity of the water a little bit by adding table salt. A 4.8% salt solution (put 48 grams of salt in a container and fill it up to one liter with water) has a specific gravity of about 1.032, closer to benzyl's 1.043. I find that the salt tends to cloud the water a bit.. you might want to experiment with other additives. (Antifreeze? Vinegar?) This is put into a closed container and placed above a 40-watt bulb, as above. Either way, I would suggest using distilled water and consider sterilising the container by immersing it in boiling water for a few minutes.. algae growing in lava lamps is not very hip. Caveat: Some of these chemicals are not good for you. Caveat 2: Some of these companies are not good for you if they find you've been infringing on their patent rights and trying to sell your new line of "magma lights." Be careful. 31.4 What is Goretex?. Goretex is a dispersion-polymerised PTFE that is patented by W.L.Gore and Associates [5]. It is classed as a stretched semi-crystalline film, and is produced by extrusion under stress ( faster take-up rate than extrusion rate ). The extrudate is stretched below the melting temperature, often in the presence of an aromatic hydrocarbon that swells the amorphous region, creating porosity. The hydrophobic nature of the PTFE means that liquid water is repelled from the pores, whereas water vapour can pass through. It is important to realise that once the PTFE pores are filled with liquid water, the fabric can allow liquid water to pass though until it is dry again. Thus Goretex-containing fabrics ( such as Nomex/Goretex - which consists of an outer aramid fabric, a central Goretex layer, and a cotton backing ) should never be used as protection from chemicals as many will pass straight through. Any water-miscible solvent ( eg alcohol ) can fill the pores, and then liquid water can displace it and continue to rapidly pass through until the fabric is fully dried out. 31.5 What causes an automobile airbag to inflate?. The final cause is the production of nitrogen from 10s of grams of sodium azide, but there are some extra chemicals involved along the way. Sodium azide is toxic, The airbag inflators are aluminium-encased units that contain an igniter (squib), gas generating pellets ( or wafers of sodium azide propellant ), and filters to screen out combustion products. The electrical signal ignites a few milligrams of initiator pyrotechnic material. The pyrotechnic material then ignites several grams of booster material, which ignites the tens of grams of sodium azide, and the azide burns very rapidly to produce nitrogen gas and sodium. The sodium azide is pelletised to control the rate of gas generation by controlling its surface area. The free sodium would form sodium hydroxide when it contacts the water in people's noses, mouths, and eyes so, to prevent this, the manufacturers mix in chemicals that will produce sodium salts ( silicates, aluminates, borates ) on combustion. Inflator units also often have a layer of matted material of alumina and silica called Fiberfrax in the particulate filter. The Fiberfrax mat reacts with most of the remaining free sodium in the generated gas. A typical reaction pathway is as follows [6];- 300C 2 NaN3 ------> 2 Na + 3 N2 10 Na + 2 KNO3 ------> K2O + 5 Na20 + N2 K2O + Na2O + SiO2 ------> alkaline silicate glass. There are apparently also corn starch and talcum powder used as lubricants in the bag, and if the bag explodes these are the powders that contaminate people - not the toxic chemicals in the inflator. One article quotes 160 grams of propellant for a drivers-side bag ( 60 litres of gas) and 450 grams for a passengers-side bags ( which are 3-5 times larger) . I suspect that may include all of the above ingredients in the igniter, but not the bag lubricants. The bag fills until it reaches slightly above atmospheric pressure, and the manufacturers now control the bag inflation speed to 90-200mph, which is less than the early models - because they were too violent and could harm occupants. The actual sequence goes something like:- 0 - Impact 15 - 20 milliseconds - sensors signal severe frontal collision. 18 - 23 milliseconds - pyrotechnic squib fired 21 - 27 milliseconds - nylon bag inflates 45 - 50 milliseconds - the driver ( who has moved forward 5 inches) slams into the fully inflated bag 85 -100 milliseconds - the driver "rides the bag down" as the air cushion deflates. Recently, there have been calls to change the crash testing procedures to allow the test dummy to be belted in, as seat belt usage is now about 67%. Having a belted dummy would permit the use of slower inflating airbags, as the deaths of 30 children ( up to Dec. 1996 ) have been attributed to the speed of inflation of the larger passenger-side bag. Early in 1997, the US NHTSA finally permitted depowering and/or disabling of passenger-side airbags. A major airbag supplier is Breed Automotive, Boonton Township, N.J. More details can be found in specialist articles [7-9], and research is continuing into alternative inflation mechanisms, such as compressed gases. There has been extensive work over the last decade on "hybrid" airbag systems. These two-stage systems often use cylinders of compressed gas, which can be released at ambient temperatures for situations where low-speed deployment is appropriate, or the gas can be rapidly heated for high-speed deployment. 31.6 How hazardous is spilt mercury?. First step - ensure any broken thermometer actually contained mercury, as many only contain alcohol. Mercury has an appreciable vapour pressure at ambient temperatures, thus if the mercury has split somewhere warm and with limited air circulation, then vapour concentrations can accumulate. When mercury drops any distance onto a surface, it splatters into hundreds of minute globules, resulting in a large surface area. The major hazard is the mercury vapour produced from the spill. Mercury usually ends up in carpet or cracks in the surface, and so really is only a significant hazard to children crawling around the floor. Do not over-react. If the location is relatively cool and well-ventilated, there is little danger to adults. Remove as much mercury as conveniently possible, and just remember when toddlers come visiting that there is a slight potential hazard if the area is not well-ventilated and is warm. Obviously, if you increase the ventilation, the concentrations will decrease faster. The USA ACGIH TLV for mercury vapour is 0.05mg/m3, whilst the DFG ( Germany ) limit is 0.01mg/m3, and the vapour pressure of mercury at 25C is 0.0018mm. At 25C, the equilibrium concentration would be about 20mg/m3, which is 400 times the permitted TLV. It is unlikely that this equilibrium would be reached in areas where there are significant airflows, unless the mercury had been finely dispersed ( as in a blown manometer, or dropped onto a very rough surface ). Mercury vapour is rapidly oxidised to divalent ionic mercury by the tissues of the body. Human volunteers exposed to tracer doses of elemental Hg demonstrated first order kinetics for excretion with a half life of 60 days. The lethal concentration for humans is apparently not known, but acute mercurialism has resulted from exposures to concentrations within the range 1.2 - 8.5mg/m3. The human organism is able to absorb and excrete substantial amounts of mercury, in some cases as high as 2 mg/day without exhibiting any abnormal symptoms or physical signs [10]. The Dietary uptake for mercury was estimated to be :- 3 micrograms/day Adults 1 " " young children 1 " " infants. and the adult uptake was estimated to comprise of 0.3 air via Hg(0), 0.1 water via Hg(2+), 3 food via Hg(CH3Hg+). ( EPA Mercury Criteria Document 1979 ) The CRC Handbook of Laboratory Safety [11] has a chapter on mercury hazards. A good discussion of mercury ( and other metals ) is found in "Metals and their Compounds in the Environment: Occurrence, Analysis and Biological Relevance" [12]. The best method of removing spilt mercury is to use a vacuum with a flask and pasteur pipette and chase the little globules around the floor while not breathing :-). Seriously, a simple vacuum system, or even a pasteur pipette, can remove most of the large globules. There are special commercial vacuum cleaners, but never use a household one - as the expelled air will contain mercury vapour, and the fine metal globules will contaminate the cleaner. For nooks, crannies, and cracks - where the mercury is likely to remain undisturbed, you can either apply flowers of sulfur ( fine elemental sulfur ) or zinc dust, with vigorous brushing to facilitate contact, and sweep up the excess. If the mercury is going to be re-exposed ( by cleaning, foot traffic etc., ), then the zinc dust may be preferred because of an apparently faster reaction rate. However, if you have a light-coloured carpet, pouring yellow or grey powder is not usually an option, and if the location is warm and not well-ventilated near ground level, ensure that toddlers do not spend hours every day playing there. There have been several studies on the best methods to clean up spills, including "Vaporisation of Mercury spillage" [13]. The abstract reports " A report on an investigation of the problem in laboratories and industries of mercury (Hg) vaporisation from small droplets in cracks and floors. The efficacy of other fixing agents besides flowers of sulfur was metered. The results show that the use of a sulfur, calcium oxide and water mixture was the most successful mixture for fixing mercury droplets. A second convenient technique is the use of an aerosol hair spray. A chelating soap is available in some countries, and this would presumably be the method of choice in dealing with spillages." Another article includes methods based on amalgamating with zinc impregnated in a metal sponge or scrubbing pad for picking up mercury [14], and another investigates substances that can be used to remove spilled mercury - such as iodised activated carbon, copper or zinc powders, molecular sieves of copper or silver ions, and silica gel [15]. Dental amalgam is apparently a finely divided powder of a silver, tin, and copper alloy that is mixed with the mercury. The setting time probably is a function of the slow dissolution of the alloy in the mercury due to the particle size of the powder used. The mass % of each individual metal amalgam when mercury is saturated at 20C is Ag = 0.04, Cu = 0.0032, and Sn = 0.62, but I've no idea if that is the ratio actually used. I presume the ratio may be varied to obtain the desired physical properties, and that there would be a theoretical excess of the alloy to ensure minimal free mercury. The actual amount of mercury vapour from dental amalgam is low, but directly measurable by sensitive mercury vapour analysers. The significance of mercury vapour from dental amalgam to health has been very controversial, however there are now practical alternatives in widespread use. 31.7 Did molasses really kill 21 people in Boston?. From: mica@world.std.com (mitchell swartz) Date: Sun, 4 Jul 1993 Subject: Molasses Accident [excerpt from the Book of Lists #3 (Wallace et alia)] THE GREAT BOSTON MOLASSES FLOOD "On Jan. 15, 1919, the workers and residents of Boston's North End, mostly Irish and Italian, were out enjoying the noontime sun of an unseasonably warm day. Suddenly, with only a low rumble of warning, the huge cast-iron tank of the Purity Distilling Company burst open and a great wave of raw black molasses, two stories high, poured down Commercial Street and oozed into the adjacent waterfront area. Neither pedestrians nor horse-drawn wagons could outrun it. Two million gallons of molasses, originally destined for rum, engulfed scores of persons - 21 men, women, and children died of drowning or suffocation, while another 150 were injured. Buildings crumbled, and an elevated train track collapsed. Those horses not completely swallowed up were so trapped in the goo they had to be shot by the police. Sightseers who came to see the chaos couldn't help but walk in the molasses. On their way home they spread the sticky substance throughout the city. Boston smelled of molasses for a week, and the harbor ran brown until summer." From this we see 21 people were killed, the half life was fairly short for the contaminants. Long term effects were probably negligible. 31.8 What is the active ingredient in mothballs?. Mothballs were originally made from camphor ( C10H16O, [76-22-2], MP 176C, BP 204C ), or naphthalene ( C10H8, [91-20-3], MP 82C, BP 218C ), but para-dichlorobenzene ( C6H4Cl2, [106-46-7], MP 55C, BP 173C ), became cheaply available as an unwanted by-product of ortho-dichlorobenzene production, and thus became the most common active ingredient. However para-dichlorobenzene is also a suspected carcinogen, and naphthalene has again become a common active ingredient. Consequently, the best method of finding the active ingredient is to read the label on the packet, Note that adding mothballs to modern gasolines will not increase the octane rating of the fuel - refer to the Gasoline FAQ posted in rec.autos.tech for more details. 31.9 Is vinegar just acetic acid?. Most countries have food regulations that permit the use of acetic acid as clearly-labelled "synthetic white vinegar". Most vinegars are actually malt vinegars ( fermented ), and synthetic acetic acid is not allowed to be sold as Malt Vinegar. Most natural, unfortified, malt vinegars are appropriately labelled. The classification can get rather messy when bulk suppliers dilute malt vinegar concentrates with acetic acid, which itself could either be synthetic, or from another fermentation process. Regulations usually require any addition of acetic acid to be clearly marked on the label, and the product is not normally legally sold as pure "malt vinegar". The amount of acetic acid in "natural" malt, cider, or wine vinegars usually ranges from 4% - 6%, but some examples can have up to approximately 20%. Vinegar is produced by the exothermic aerobic bacterial oxidation of ethanol to acetic acid via acetaldehyde. 31.10 What are the different grades of laboratory water?. There are several techniques used in chemical laboratories to obtain the required purity of water. There are several grading systems for water, but the most well-known is the ASTM system, although certain applications (HPLC) often require purer water than ASTM Type I, consequently additional treatments such as ultrafiltration and UV oxidation may also be used to reduce concentrations of uncontrolled impurities, such as organics. ASTM Type I II III Specific Conductance (max. uMhos/cm.) <0.06 <1.0 <1.0 Specific Resistance (min. Mohms/cm.) >16.67 >1.0 >1.0 Total Matter ( max. mg/l ) <0.1 <0.1 <1.0 Silicate ( max. mg/l ) N/D N/D 0.01 KMnO4 Reduction ( min. mins ) >60.0 >60.0 >10.0 Type A B C Colony Count (Colony forming units/ml) 0 Bacteria <10 <100 pH NA NA 6.2-7.5 The techniques to purify natural waters - which may be almost saturated with some contaminants - are frequently used in combination to obtain high purity laboratory water. Some purification techniques use less energy than distilling the water, and may be used in combination where large volumes of "pure" water are required. The design of purified water systems, and the materials used for construction, are selected according to the important contaminants of the water. For some applications, 316L stainless steel may be required, whereas other applications may require polyvinylidene difluoride and polytetrafluoroethylene materials. Systems are carefully designed to minimise the volume of water remaining static and in "dead ends" - where microbes could grow. The first treatment is usually a coarse physical filtration using a depth filter that can remove undissolved large particles and other insoluble material in the feed water. For smaller volumes, distillation is the pretreatment method of choice. Distilled water is water that has been boiled in a still and the vapour condensed to obtained distilled water. While many impurities are removed ( especially dissolved and undissolved inorganics that make water "hard", most organisms, etc. ), some impurities do remain ( volatile and some non-volatile organics, dissolved gases, and trace quantities of fine particulates ). Distilled water has lost many of the ionic species that provided a pH buffer effect so, as it dissolves some CO2 from the air during condensation and storage, the pH moves to around 5.5 ( usually from close to the neutral pH of 7.0 ). Distilled water has the vast majority of impurities removed, but often those residual compounds still make it unsuitable for demanding applications, so there are alternative methods of purifying water to remove specific undesirable species. The next common treatment is ion-exchange, which involves using a bed of resin that exchanges with unwanted dissolved species, such as those that cause "hardness" ( calcium, magnesium ) in water. Two resins are used, one that exchanges anions ( usually a strong anion exchanger such as Amberlite IRA-400 - a quaternary ammonium compound on polystyrene ), and one that exchanges cations ( usually a strong cation exchanger such as Amberlite IR-120 - a sulfonic acid compound on polystyrene ). These resins can also be combined in "mixed bed" resins, such as Amberlite MB-1A, which is a mixture of IRA-400 [OH- form] and IR-120 [H+ form]. The porosity of the polystyrene-based resin is dependant on the amount of cross-linking, which is, in turn, dependant on the proportion of divinyl benzene used in the process. Unfortunately, selectivity of a highly porous resin is inferior to that of a less porous, more cross-linked, resin, so a balance between the rate of exchange and the selectivity is sought. Agarose, cellulose, or dextran can be used in place of the polystyrene base. Sophisticated systems can have many beds in sequence, using both stronger and weaker ion exchange resins. The exchange potential for ions depends on a number of factors, including molecular size, valency and concentration. In dilute solutions, exchange potentials increase with increasing valency, but in concentrated solutions the effect of valency is reversed, favouring the absorption of univalent ions rather than polyvalent ions. This explains why calcium and magnesium can be strongly absorbed from feedwater in softening processes, but then are easily removed from the ion exchange resin when concentrated sodium chloride is used as regenerant. In dilute solutions, the order of common anion exchange potentials on strong anion exchangers is sulfate > chromate > citrate > nitrate > phosphate > iodide > chloride. In dilute solutions, the order of common cation exchange potentials on strong cation exchangers is Fe3+ > Al2+ > Ba2+ > Pb2+ > Ca2+ > Cu2+ > Zn2+ = Mg2+ > NH4+ = K+ > Na+ > H+ > Hg2+. There are two forms of ion exchange for water purification. To "deionise" feed water, the resins are in the OH- ( anion exchanger ) and H+ ( cation exchanger ) forms. If sodium chloride was present in the feed water, the sodium ion would displace the hydrogen ion from the cation resin, while the chloride would displace the hydroxyl ion from the anion resin. The displaced ions can combine to form water. Separate beds of resins can be regenerated using 1 Normal acid ( HCl or H2SO4 ) for strongly-acid cation resins, or 1 Normal sodium hydroxide for strongly-basic anion resins. The amount of regenerant is approximately 150 - 500% of the theoretical exchange capacity of the bed. If the intention is to merely "soften" the feed water to reduce deposits, the beds can be in the Cl- ( anion exchanger ) and Na+ ( cation exchanger ) forms. These are replaced by the dilute polyvalent species in the water that rapidly form undesirable insoluble deposits as process water evaporates, like calcium, magnesium and sulfate. The beds can be regenerated by passing highly concentrated salt ( sodium chloride ) solutions through them until all the polyvalent ions on the resins have been replaced. This technique produces "soft" process water that used in industry. When a dilute feedwater solution containing salt passes through a cation exchange resin bed in the hydrogen form, the reaction that occurs is:- Na+ + Cl + R.SO3H <=> H+ + Cl- + R.SO3Na Obviously, the acidity of the water strongly increases as it moves down the bed, which inhibits the exchange process. If a mixed bed is used, the products soon encounter the anion exchange resin and are also removed:- H+ + Cl- + R.NH2 <=> R.NH3 + Cl- H+ + Cl- + R.NH3OH <=> R.NH3 + Cl- + H2O Mixed bed resins are usually more efficient than equivalent single beds. If the water feeding the resin beds has already been distilled ( very common in laboratories - the resin beds then last much, much longer, and the distillation has also removed other impurities ), then the water is called "distilled and deionised". Laboratory water that has had most of the ionic impurities removed will have a high electrical resistance, and is often known as "18.3 megohm" water because the electrical resistance is >18,300,000 ohm/cm, but note that non-ionic impurities may still be present. An alternative process that has increasingly replaced ion-exchange is reverse-osmosis, which uses osmotic pressure across special membranes to remove most of the impurities. It is called reverse-osmosis because the feed side is pressurised to drive the purified water through the membrane in the opposite direction than would occur if both sides were the same pressure. The two common membrane materials are cellulose acetate or polysulfone coated with polyamine, and typical rejection characteristics are:- Monovalent Divalent Pyrogens, Bacteria Ions Ions Organics > 200 MW Cellulose Acetate >88% >94% >99% Polyamine >90% >95% >99% The huge advantage of RO is that membranes can easily be maintained ( occasional chemical sterilisations ), are largely self-cleaning, and can produce large amounts of water with no chemical regeneration and minimal energy requirements - just the pressure ( 200 psi ) required to push the water along the membrane surfaces and improve the osmotic yield. RO is commonly used as a pretreatment stage when very pure water is required, and for situations where large volumes of reasonably pure water are required. Organic species and free chlorine are usually removed from water by passing the water through a bed of activated carbon where they form a low energy chemical link with the carbon. These filters are often installed upstream of the ion-exchange and reverse osmosis stages to protect them from chlorine and organics in the feed water. Polyamine RO membranes require feedwater containing <0.1ppm free chlorine, however cellulose acetate membranes can tolerate up to 1.5ppm free chlorine. The final stage of producing "pure" laboratory water usually involves passing the deionised water through a 0.22um filter, which is sufficiently small to remove the vast majority of organisms ( the smallest known bacterium is around 0.3um ), thus sterilising the water. Recently, ultrafiltration has become popular as a means of reducing pyrogens ( they are usually lipopolysaccharides from the degradation of gram negative bacteria ). They are measured by either injecting a sample into test rabbits and measuring body temperature increase or by the more sensitive Limulus Amebocyte Lysate (LAL) test. The internal membrane of an ultrafiltration system has a pore size of <0.005um. This will remove most particles, colloidal silica, and high MW organics such as pyrogens, down to about 10,000MW. These are usually for cell-culture and DNA research, and are located at the point of use, however the ultrafiltration unit has to be regularly sanitized to prevent microbial growth. Ultraviolet irradiation can be used as a bactericide (254nm) or to destroy organics by photo-oxidation (185nm). The water is exposed to UV for periods up to 30 minutes, and the UV interacts with dissolved oxygen to produce ozone. The ozone promotes hydroxyl radical formation, which result in the destruction of organic material. Usually a high intensity, quartz mercury vapour lamp is used, and is followed by an ion exchange and organic scavenger cartridge to collect decomposition products. The product water is very low in total organic carbon. Dissolved gases can be removed by passing the water through a vacuum degassing module that utilises an inert, gas-permeable membrane surrounded by a vacuum to remove dissolved gases from the water. The purest laboratory water is usually obtained after passing through a system that can include reverse osmosis or distillation of the feed water, followed by activated carbon to remove chlorine and organics. The water is passed through ion exchange resins to remove inorganic ions, through a UV oxidation stage, followed by a combined ion exchange and organic scavenger cartridge, and finally through a 0.22um filter. An additional stage of vacuum degassing to remove dissolved gases may be added for some applications - such as for semiconductor production. These pure water systems are regarded as " point-of-use ", because it is extremely difficult to prevent the reintroduction of contamination during storage and distribution. The water is commonly known as " 18.3 Megohm " water, because it has a specific resistance greater than 18.3 Megohm-cm at 25C. It also contains < 5 ppb of total organic carbon, < 10 ppb of total dissolved solids, and < 1 colony forming unit / mL of micro-organisms. Details of laboratory and industrial water-purification processes are available in the catalogues of equipment suppliers such as Barnstead [16] and Millipore [17]. User Contributions:Top Document: Sci.chem FAQ - Part 6 of 7 Previous Document: 30. Polymer Chemistry Part1 - Part2 - Part3 - Part4 - Part5 - Part6 - Part7 - Single Page [ Usenet FAQs | Web FAQs | Documents | RFC Index ] Send corrections/additions to the FAQ Maintainer: B.Hamilton@irl.cri.nz
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