Patent application number | Description | Published |
20090077329 | Non-broadcast signature-based transactional memory - A coherence controller in hardware of an apparatus in an example detects conflicts on coherence requests through direct, non-broadcast employment of signatures that: summarize read-sets and write-sets of memory transactions; and provide false positives but no false negatives for the conflicts on the coherence requests. The signatures comprise fixed-size representations of a substantially arbitrary set of addresses for the read-sets and the write-sets of the memory transactions. | 03-19-2009 |
20140108731 | Energy Optimized Cache Memory Architecture Exploiting Spatial Locality - Aspects of the present invention provide a “SuperTag” cache that manages cache at three granularities: (i) coarse grain, multi-block “super blocks,” (ii) single cache blocks and (iii) fine grain, fractional block “data segments.” Since contiguous blocks have the same tag address, by tracking multi-block super blocks, the SuperTag cache inherently increases per-block tag space, allowing higher compressibility without incurring high area overheads. To improve compression ratio, the SuperTag cache uses variable-packing compression allowing variable-size compressed blocks without requiring costly compactions. The SuperTag cache also stores data segments dynamically. In addition, the SuperTag cache is able to further improve the compression ratio by co-compressing contiguous blocks. As a result, the Super Tag cache improves energy and performance for memory intensive applications over conventional compressed caches. | 04-17-2014 |
20150192980 | Electronic Computer Providing Power/Performance Management - Computational resources of an electronic computer are monitored by predictors that establish predicted trade-offs between performance and power for a particular workload while the workload is being executed. A coordinator combines prediction values to identify a limited number of combinations of operating states of the computational resources, allowing operating states of the computational resources to be readily adapted during program execution based on a particular workload. The limited number of combinations of operating states are ideally Pareto optimal combinations. | 07-09-2015 |
20150363903 | Wavefront Resource Virtualization - A processor comprising hardware logic configured to execute of a first wavefront in a hardware resource and stop execution of the first wavefront before the first wavefront completes. The processor schedules a second wavefront for execution in the hardware resource. | 12-17-2015 |
Patent application number | Description | Published |
20080206798 | KITS FOR DETECTION OF ATP - Methods and kits for detecting the presence of ATP, for measuring ATP concentrations, and for detecting viable cells using a composition comprising an ATP-dependent enzyme and one or more ATPase inhibitors. | 08-28-2008 |
20140194307 | RECOGNITION OF CELLULAR TARGET BINDING BY A BIOACTIVE AGENT USING INTRACELLULAR BIOLUMINESCENCE RESONANCE ENERGY TRANSFER - The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, or portions, components, or subunits of bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith. | 07-10-2014 |
20140194325 | RECOGNITION OF CELLULAR TARGET BINDING BY A BIOACTIVE AGENT USING INTRACELLULAR BIOLUMINESCENCE RESONANCE ENERGY TRANSFER - The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith. | 07-10-2014 |
20140199712 | COMPOSITIONS AND METHODS FOR CAPTURE OF CELLULAR TARGETS OF BIOACTIVE AGENTS - The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith. | 07-17-2014 |
20140287427 | COMPOSITIONS AND METHODS FOR CAPTURE OF CELLULAR TARGETS OF BIOACTIVE AGENTS - The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith. | 09-25-2014 |
20140348747 | ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION - Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide. | 11-27-2014 |
20140363375 | ACTIVATION OF BIOLUMINESCENCE BY STRUCTURAL COMPLEMENTATION - Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide. | 12-11-2014 |
20160097042 | INTERNAL PROTEIN TAGS - Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex). | 04-07-2016 |
Patent application number | Description | Published |
20090137019 | Thermostable luciferases and methods of production - Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits. | 05-28-2009 |
20110003316 | LUMINESCENCE-BASED METHODS AND PROBES FOR MEASURING CYTOCHROME P450 ACTIVITY - The present invention provides methods, compositions, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferin or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. Upon addition of the luciferin derivative or other luminogenic molecule into a P450 reaction, the P450 enzyme metabolizes the molecule into a bioluminescent enzyme substrate, e.g., luciferin and/or luciferin derivative metabolite, in a P450 reaction. The resulting metabolite(s) serves as a substrate of the bioluminescent enzyme, e.g., luciferase, in a second light-generating reaction. Luminescent cytochrome P450 assays with low background signals and high sensitivity are disclosed and isoform selectivity is demonstrated. The present invention also provides an improved method for performing luciferase reactions which employs added pyrophosphatase to remove inorganic pyrophosphate, a luciferase inhibitor which may be present in the reaction mixture as a contaminant or may be generated during the reaction. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors. | 01-06-2011 |
20110081670 | LUCIFERASE-BASED ASSAYS - A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent. | 04-07-2011 |
20120174242 | OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE - An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type | 07-05-2012 |
20130217051 | LUCIFERASE-BASED ASSAYS - A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent. | 08-22-2013 |
20130217052 | LUCIFERASE-BASED ASSAYS - A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent. | 08-22-2013 |
20140223590 | OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE - An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type | 08-07-2014 |
20150337198 | COMPOSITIONS AND METHODS FOR STABILIZING LUMINOGENIC SUBSTRATES - Disclosed herein are compositions and methods for stabilizing a luminogenic substrate such as coelenterazine or a functional analog thereof. The functional analog may be furimazine. The composition may include the luminogenic substrate, a thionucleoside, and an organic solvent, in which the thionucleoside is present in an amount effective to stabilize the luminogenic composition against decomposition. The method provided herein stabilizes the luminogenic substrate against decomposition by contacting the luminogenic substrate with an effective amount of the thionucleoside in the presence of the organic solvent. Also provided herein is a kit containing the composition. | 11-26-2015 |
Patent application number | Description | Published |
20080277522 | POLYMERIC FILM WINDING SYSTEMS AND METHODS UTILIZING INK SPACING - A method and system of winding polymeric film includes printing an ink pattern onto a length of polymeric film (e.g., low modulus, optical grade film) prior to winding. The film and the ink pattern are simultaneously wound about a cylinder to form a wound roll, with ink pattern being interposed between successively wound layers of film. The ink pattern establishes a gap between the outermost and immediately underlying wound layers of film. The gap facilitates natural movement (e.g., relaxation) of the outermost wound layer relative to the immediately underlying wound layer, thereby compensating for any winding-related stress or tension. | 11-13-2008 |
20130133770 | APPARATUS AND METHOD FOR REPAIRING DAMAGED PIPES - An induction heating device for heating the surface of a pipe or pipeline comprises an induction heating head ( | 05-30-2013 |
20130299418 | METHOD OF FORMING FILTER ELEMENTS - A method of forming a filter element is disclosed comprising introducing a mixture into a feeder, the mixture comprising a plurality of susceptor particles and a plurality of polymeric binder particles. The mixture is advanced through the feeder and into a die comprising an excitation portion where eddy currents are induced in the susceptor particles by subjecting the mixture to a high-frequency electromagnetic field, the eddy currents being sufficient to elevate the temperature of the susceptor particles to cause adjacent polymeric binder particles to be heated to at least a softening point. The susceptor particles bind with the heated polymeric binder particles in the die to form a coherent mass. The coherent mass is advanced out of the die and cooled to form the filter element. | 11-14-2013 |
20150079337 | FILMS COMPRISING AN ARRAY OF OPENINGS AND METHODS OF MAKING THE SAME - Polymeric layer having first and second, generally opposed major surfaces, comprising an array of openings extending between the first and second major surfaces. The polymeric layers are useful, for example, as components in personal care garments such as diapers and feminine hygiene products. They can also be useful for filtering (including liquid filtering) and acoustic applications. | 03-19-2015 |