Patent application number | Description | Published |
20080277494 | Liquid Bridge and System | 11-13-2008 |
20080280331 | Microfluidic Analysis System - A thermal cycling device ( | 11-13-2008 |
20100029512 | FORMING SAMPLE COMBINATIONS USING LIQUID BRIDGE SYSTEMS - The present invention generally relates to methods of constructing liquid bridges and methods of forming predetermined combinations of samples using liquid bridges. | 02-04-2010 |
20100041055 | NOVEL GENE NORMALIZATION METHODS - Measurement of gene expression relative to an endogenous control gene is prone to excessive variability between samples and even replicates. The disclosure provides methods for normalizing expression levels of a gene by scaling gene expression levels to that of the most highly expressed gene in the set of genes whose expression levels are measured, rather than a house-keeping gene. | 02-18-2010 |
20100092973 | METHODS AND DEVICES FOR DIGITAL PCR - The invention provides methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a “droplet-in-oil” technology. In the methods, the starting sampled is segmented at least partially into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point. | 04-15-2010 |
20100092987 | MICROFLUIDIC ANALYSIS SYSTEM - A microfluidic analysis system ( | 04-15-2010 |
20100120635 | SAMPLE DISPENSING - The present invention generally relates to systems and methods for mixing and dispensing a sample droplet from a microfluidic system, such as a liquid bridge system. In certain embodiments, the invention provides systems for mixing and dispensing sample droplets, including a sample acquisition stage, a device for mixing sample droplets to form sample droplets wrapped in an immiscible carrier fluid, a dispensing port, and at least one channel connecting the stage, the droplet mixing device, and the port, in which the system is configured to establish a siphoning effect for dispensing the droplets. | 05-13-2010 |
20100216128 | METHODS FOR ANALYZING AGRICULTURAL AND ENVIRONMENTAL SAMPLES - The present invention generally relates methods for analyzing agricultural and/or environmental samples using liquid bridges. In certain embodiments, the invention provides a method for analyzing an agricultural sample for a desired trait including obtaining a gene or gene product from an agricultural sample, in which the gene or gene product is in a first fluid; providing a liquid bridge for mixing the gene or gene product with at least one reagent to form a mixed droplet that is wrapped in an immiscible second fluid; and analyzing the mixed droplet to detect a desired trait of the agricultural sample. | 08-26-2010 |
20100297685 | METHODS FOR CULTURING AND ANALYZING CELLS - The present invention generally relates to methods for culturing and analyzing cells using liquid bridges. | 11-25-2010 |
20100297748 | INTEGRATED FLUIDIC CIRCUITS - The invention generally relates to an integrated fluidic circuit. In certain embodiments, the circuit includes a main chamber having a withdrawal port, a carrier fluid occupying a volume in the chamber, in which the carrier fluid is immiscible with a sample fluid, and a plurality of liquid bridges disposed within the chamber. | 11-25-2010 |
20100304443 | SAMPLING DEVICE - The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath. | 12-02-2010 |
20100304446 | DEVICES, SYSTEMS, AND METHODS FOR AMPLIFYING NUCLEIC ACIDS - The present invention generally relates to a devices, systems, and methods for amplifying nucleic acids in flowing droplets. In certain embodiments, the invention provides a device for amplifying nucleic acids including at least one channel through which sample droplets including nucleic acids flow, in which the nucleic acids in the droplets are optically detectable while the droplets are flowing through the channel, and a plurality of temperature zones in thermal contact with the channel, in which the zones are located at different locations along the channel and the zones are separated from each other. | 12-02-2010 |
20120003658 | MICROFLUIDIC DROPLET QUEUING NETWORK - A multi-port liquid bridge ( | 01-05-2012 |
20140038196 | Forming Sample Combinations Using Liquid Bridge Systems - The present invention generally relates to methods of constructing liquid bridges and methods of forming predetermined combinations of samples using liquid bridges. | 02-06-2014 |
20140045185 | Novel Gene Normalization Methods - Measurement of gene expression relative to an endogenous control gene is prone to excessive variability between samples and even replicates. The disclosure provides methods for normalizing expression levels of a gene by scaling gene expression levels to that of the most highly expressed gene in the set of genes whose expression levels are measured, rather than a house-keeping gene. | 02-13-2014 |
20140051604 | MICROFLUIDIC DROPLET QUEUING NETWORK - A multi-port liquid bridge ( | 02-20-2014 |
20140096833 | Microfluidic Connector - A microfluidic connector comprises an enclosure, a fluidic inlet port and a fluidic outlet port, in the enclosure, in which the inlet and outlet ports are movable with respect to each other, for example, mutual spacing between the inlet and outlet ports is variable. A port is in a fixed part of the enclosure, and another port is in a part of the enclosure which slides with respect to the fixed part. There may be multiple inlet ports and/or multiple outlet ports. Also, there may be an auxiliary port for introduction of fluid into the enclosure or removal of fluid from the enclosure. | 04-10-2014 |
20140120604 | Biological Detection System and Method of Use - Provided herein is a biological detection system and method of use wherein the biological detection system comprises at least one mixer or liquid bridge for combining at least two liquid droplets and an error correction system for detecting whether or not proper mixing or combining of the two component droplets have occurred. | 05-01-2014 |
20140349387 | Sampling Device - The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath. | 11-27-2014 |