Patent application number | Description | Published |
20090104682 | Construction of novel strains containing minimizing genome by tn5-coupled cre/loxp excision system - Disclosed is a method for developing novel strains deleted specific chromosome sites, using transposon and Cre/loxP site-specific recombination by Cre expression vector, wherein the transposon comprises a selectable marker and loxP site. The method comprises the steps of: (1) preparing a transposon comprising a selectable marker and loxP site; (2) inserting the transposon into an optional position of microbial chromosome, and determining the inserted site; (3) integrating two transposons comprising a different selectable marker to one chromosome; (4) deleting a chromosomal site between the two lox sites by introducing a Cre expression vector into the chromosome of step (3); and (5) repeating steps (3 and 4) for the mutant deleted a part of chromosome, to shorten the chromosome of mutant gradually. | 04-23-2009 |
20090305421 | Recombinant vector for deleting specific regions of chromosome and method for deleting specific chromosomal regions of chromosome in the microorganism using the same - Disclosed herein are a recombinant vector for deletion of specific chromosomal regions and a method for deletion of targeted microbial chromosomal regions using the same. Specifically, the recombinant vector comprises an arabinose-inducible promoter; a gene encoding a protein involved in lambda (λ)-red recombination; a rhamnose-inducible promoter; and a gene encoding the I-SceI endonuclease. The present invention enables a convenient, rapid and markerless successive deletion of specific genes of microbes, as compared to a conventional method. | 12-10-2009 |
20090312249 | TLR4 decoy receptor protein - The present application discloses a nucleic acid molecule encoding a fusion polypeptide capable of binding myeloid differentiation protein-2 (MD-2) polypeptide, which includes: a nucleotide sequence encoding an MD-2 polypeptide-binding portion of human toll-like receptor 4 (TLR4), a leucine-rich repeats (LRR) module, and a multimerizing component. | 12-17-2009 |
20100136663 | PREPARATION OF AN ARTIFICIAL TRANSCRIPTION FACTOR COMPRISING ZINC FINGER PROTEIN AND TRANSCRIPTION FACTOR OF PROKARYOTE, AND A USE THEREOF - The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an | 06-03-2010 |
20100184949 | Method for the mass expression of an antimicrobial peptide by using a translational coupling system - The present invention relates to a gene construct which is capable of achieving efficient production of an antimicrobial peptide in a microorganism, and a method for efficient mass production and separation of an antimicrobial peptide using the same. The gene construct of the present invention has a translationally coupled configuration of two independent and separate cistrons which encode an acidic peptide and a basic antimicrobial peptide, each having an opposite charge, under the control of a single promoter. The translationally coupled acidic peptide and basic antimicrobial peptide undergo charge-charge interaction simultaneously with expression thereof to neutralize the potential cytotoxicity of the antimicrobial peptide, resulting in prevention of antimicrobial peptide-mediated killing of host microorganisms. In addition, a conjugate of the acidic peptide and the antimicrobial peptide can be separated without chemical or enzymatic treatment. Therefore, it is possible to achieve easy mass production of antimicrobial peptides from recombinant microorganisms. | 07-22-2010 |
20100330623 | COMPOSITIONS AND METHODS FOR AMINO ACID BIOSYNTHESIS - The present invention relates generally to compositions and methods useful for, inter alia, production of commercial biologic products such as amino acids. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products. The present invention also provides, inter alia, novel isolated DNA, nucleic acid, vectors and reduced genome bacteria. | 12-30-2010 |
20120088733 | NOVEL USE OF ANTIMICROBIAL PEPTIDES IN REGENERATION OF SKIN CELLS - Disclosed are novel antimicrobial peptides which can promote the regeneration of skin cells, thus healing wounds. Pharmaceutical compositions comprising the peptides as active ingredients are also provided for wound healing and skin rejuvenation. | 04-12-2012 |
20120264167 | NOVEL GINSENOSIDE GLYCOSIDASE DERIVED FROM THE GENUS TERRABACTER, AND USE THEREOF - The present invention relates to a novel ginsenoside glycosidase protein derived from the genus | 10-18-2012 |
20130244949 | NOVEL ANTICANCER AGENTS COMPRISING PEPTIDES WITH CANCER-SPECIFIC TOXICITY - The present invention relates a prophylactic or therapeutic composition for cancer, and more particularly, to a pro-phylactic or therapeutic composition for cancer comprising a peptide which is represented by an amino acid sequence of the following Formula (I), a method for preventing or treating cancer comprising the step of administering the peptide to a subject, and use of the peptide in the preparation of the prophylactic or therapeutic composition for cancer. (I) APKAMX | 09-19-2013 |
20140162331 | AMPHIPATHIC PEPTIDE-LIPASE CONJUGATE HAVING ADVANCED LIPASE ACTIVITY AND USE THEREOF - Disclosed are an amphipathic peptide-lipase conjugate with enhanced lipase activity, a polynucleotide coding for the conjugate, an expression vector carrying the polynucleotide, a transformant anchoring the expression vector therein, a method for preparing the conjugate, a lipolysis method using the conjugate, and a method for producing biodiesel using the lipase. | 06-12-2014 |
20150044712 | METHOD AND KIT FOR ANALYZING PROTEIN-PROTEIN INTERACTION USING NANOCLUSTER FORMATION - For efficient analysis of a protein-protein interaction, the present disclosure provides a kit for analyzing a protein-protein interaction, the kit including: a 1 | 02-12-2015 |