Patent application number | Description | Published |
20080251383 | Microfluidic Liquid Stream Configuration System - A microfluidic liquid stream configuration system is provided including providing a substrate; forming a first co-planar electrode and a second co-planar electrode on the substrate; applying a dielectric layer, with a controlled surface energy, on the first co-planar electrode and the second co-planar electrode; forming an input reservoir on the first co-planar electrode and a second co-planar electrode; supplying a liquid in the input reservoir for analysis; and imposing an electric field, an electric field gradient, or a combination thereof on the liquid for respectively driving surface charge or dipole moments in the liquid for configuring a liquid stream. | 10-16-2008 |
20090269243 | ELECTRICALLY CONTROLLED MICROFLUIDIC SYSTEM - An electrically controlled microfluidic system ( | 10-29-2009 |
20100035290 | ENZYME DETECTION SYSTEM WITH CAGED SUBSTRATES - An enzyme detection method includes forming a caged substrate; releasing an uncaged substrate by cleaving a caging molecules from the caged substrate; and emitting a light emission from a Bioluminescence Resonance Energy Transfer luminescent nanocrystal conjugate reacting with the uncaged substrate. | 02-11-2010 |
20100055725 | SYSTEM FOR ASSAYS OF AMINOTRANSFERASE - An assay system ( | 03-04-2010 |
20100068741 | ASSAY SYSTEM FOR ADENOSINE TRIPHOSPHATE AND CREATINE KINASE - An assay method includes providing a luminescent nanocrystal; combining a solution having an adenosine triphosphate molecule; and displaying a light emission by the luminescent nanocrystal and the solution combined. | 03-18-2010 |
Patent application number | Description | Published |
20130273520 | COMPOSITION, DEVICE, AND METHOD FOR BIOLOGICAL AIR SAMPLING - The present invention generally relates to air sampling of biological compounds. Specifically, the present invention relates to a device and method for sampling the ambient air for detecting microbial propagules, microbial propagules being any spore, vegetative cell, or virion of microbiological origin including all bacteria, fungi, viruses, protozoans, molds, slime molds, chlamydospores, hyphae, and cysts. | 10-17-2013 |
20160032352 | Composition, Device, and Method for Biological Air Sampling - The present invention generally relates to air sampling of biological compounds. Specifically, the present invention relates to a device and method for sampling the ambient air for detecting microbial propagules, microbial propagules being any spore, vegetative cell, or virion of microbiological origin including all bacteria, fungi, viruses, protozoans, molds, slime molds, chlamydospores, hyphae, and cysts. | 02-04-2016 |
Patent application number | Description | Published |
20160076068 | QUANTITATIVE CONTROL OF SIALYLATION - The present disclosure is directed to the use of certain glycosyltransferase variants having N-terminal truncation deletions. Contrary to previous findings certain truncations were found to exhibit sialidase enzymatic activity, particularly a variant of human sialyltransferase (hST6Gal-I) with a truncation deletion involving the first 89 N-terminal amino acids of the respective wild-type polypeptide. A fundamental finding documented in the present disclosure is that there exists a variant of this enzyme which is capable of catalyzing transfer of a glycosyl moiety as well as hydrolysis thereof. Thus, disclosed is a specific exemplary variant of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variant of mammalian glycosyltransferase and use thereof, particularly for sialylating in a quantitatively controlled manner terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins. | 03-17-2016 |
20160102298 | N-TERMINALLY TRUNCATED GLYCOSYLTRANSFERASES - The present disclosure is directed to glycosyltransferase variants having N-terminal truncation deletions. Contrary to previous findings certain truncations comprising the conserved amino acid motif (“QVWxKDS”) were found to be compatible with glycosyltransferase enzymatic activity, particularly in a human sialyltransferase (hST6Gal-I). Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins. | 04-14-2016 |
20160102333 | QUANTITATIVE CONTROL OF SIALYLATION AND SPECIFIC MONO-SIALYLATION - The present disclosure is directed to the use of certain glycosyltransferase variants having N-terminal truncation deletions. It was found that the combination of two different truncation variants of human β-galactoside-α-2,6-sialyltransferase I (hST6Gal-I) exhibited different specific sialyltransferase enzymatic activities. In one example, under conditions wherein the first variant Δ89 hST6Gal-I catalyzed formation of bi-sialylated target molecules the second variant Δ108 hST6Gal-I catalyzed formation of mono-sialylated target molecules. Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating in a quantitatively controlled manner terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins. | 04-14-2016 |
Patent application number | Description | Published |
20100159529 | DRY COMPOSITION OF REACTION COMPOUNDS WITH STABILIZED POLMERASE - The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (PCR) amplification after re-solubilization. | 06-24-2010 |
20100273235 | STABILIZED AQUEOUS ALPHA-GALACTOSIDASE COMPOSITION AND METHODS RELATING THERETO - The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising the protein with enzymatic activity of alpha-galactosidase. | 10-28-2010 |
20110250598 | DETERGENT FREE POLYMERASES - The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step. | 10-13-2011 |
20110256589 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 10-20-2011 |
20120252071 | T7 RNA POLYMERASE VARIANTS WITH CYSTEINE-SERINE SUBSTITUTIONS - The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference. | 10-04-2012 |
20140134633 | DETERGENT FREE POLYMERASES - The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step. | 05-15-2014 |
20140162267 | DRY COMPOSITION OF REACTION COMPOUNDS WITH STABILIZED POLYMERASE - The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (PCR) amplification after re-solubilization. | 06-12-2014 |
20160032260 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 02-04-2016 |
20160032261 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 02-04-2016 |
Patent application number | Description | Published |
20110029396 | METHODS AND SYSTEMS FOR PROCESSING, ACCOUNTING, AND ADMINISTRATION OF STORED VALUE CARDS - Methods are described for accounting, administration and processing of transactions utilizing a stored value card utilizing an EFT service gateway and providing balances for the cards across a plurality of point of sale devices. One method includes receiving a message generated by the point of sale system of a merchant and parsing the message into data elements. The method also includes routing the message through a switch controller to an account holder database based on an institution ID and a branch ID, accessing an account within the database utilizing the account key, and determining if an account holder's monetary balance in the account is greater than or equal to the transaction amount. A message is formatted and one of an authorization approval or a denial based upon the determination is transmitted within the message. | 02-03-2011 |
20120221422 | METHOD AND SYSTEM FOR ACTIVATION AND FUNDING OF PREPAID CARD ACCOUNTS WITHIN A RESTRICTED AUTHORIZATION NETWORK - A method for activating and funding a stored value, prepaid card account includes acquiring, with a point of sale (POS) device, a first data set associated with the card and purchase thereof, and a second data set associated with the card and activation, deactivation, funding, loading, and reloading thereof. The method also includes transmitting a specific transaction trigger amount for card account activation and transmitting the second data set across a universal payment network to an authorization system associated with the second data set. The method further includes transmitting, from the authorization system, a transaction approval message to the POS including data relating to a funded value of the account, an electronic settlement message causing payment to the merchant for selling the card, an electronic message in the amount of the funded value of the account plus the specific transaction trigger amount, and an electronic message to debit the account. | 08-30-2012 |