Patent application number | Description | Published |
20080199907 | Method For Preparing a Target Protein Using the Shsps - The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sIISPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases. | 08-21-2008 |
20080206814 | Method For Extracellular Production of Target Proteins By Co-Expression of Ompf and Target Proteins - The present invention relates to a method for secreting and producing a target protein into cell culture broth. More particularly, the invention relates to a microorganism co-transformed with a recombinant expression vector containing | 08-28-2008 |
20080220490 | Method for Improving Organisms Using Flux Scanning Based on Enforced Objective Flux - The present invention relates to a method for improving useful substance-producing organisms using metabolic flux analysis, and more particularly to a method for improving a host organism producing a useful substance, the method comprising: calculating a maximum flux value corresponding to the theoretical maximum yield of the useful substance in the metabolic network model of the host organism for producing useful substance, and calculating the optimum value of metabolic flux associated with useful substance production in the metabolic network when the value of cell growth-associated metabolic flux is the maximum under the condition where fermentation data are applied or not applied; selecting metabolic fluxes whose absolute values increase from the range between the maximum value and the optimum value; screening genes associated with the selected metabolic fluxes; and introducing and/or amplifying the selected genes in the host organism. According to the invention, the production of the useful substance can be effectively improved by selecting metabolic fluxes to be amplified and genes involved in the metabolic fluxes from the range between the optimum value and maximum value of production-associated metabolic flux in the host organism for producing the useful substance, whose genome-level metabolic network model is constructed, and introducing and/or amplifying the selected genes in the organism. | 09-11-2008 |
20090053779 | MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME - The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine. | 02-26-2009 |
20090075352 | Method For Improving A Strain Based On In-Silico Analysis - The present invention is related to a method for improving a strain on the basis of in silico analysis, in which it compares the genomic information of a target strain for producing a useful substance to the genomic information of a strain overproducing the useful substance so as to primarily screen genes unnecessary for the overproduction of the useful substance, and then to secondarily screen genes to be deleted through performing simulation with metabolic flux analysis. According to the present invention, an improved strain can be effectively constructed by the metabolic and genetic engineering approach comprising comparatively analyzing the genomic information of a target strain for producing a useful substance and the genomic information of a strain producing a large amount of the useful substance to screen candidate genes and performing in silico simulation on the screened candidate genes to select a combination of genes to be deleted, which shows an improvement in the production of the useful substance. Accordingly, the time, effort and cost required for an actual wet test can be significantly reduced. | 03-19-2009 |
20090136587 | PHARMACEUTICAL COMPOSITION FOR THE TREATMENT OF NERVE DAMAGE COMPRISING BLOOD PLASMA OR SERUM - The present invention relates to a pharmaceutical composition for the treatment of nerve damage, and more particularly to a pharmaceutical composition for the treatment of nerve damage, which contains blood plasma or serum as an active ingredient. The inventive composition regenerates nerve cells after spinal nerve damage and provides complete structural continuity in the spinal nerve lesion sites. Thus, the composition is useful for the treatment of nerve damage. | 05-28-2009 |
20090142804 | Process For Preparing Serine-Rich Protein Employing Cysteine Synthase (CYSK) Gene - The present invention relates to a process for preparing a serine-rich foreign protein comprising culturing a bacterium containing the cysteine synthase (cysK) gene and a gene encoding the serine-rich foreign protein. The present invention comprises the steps of culturing a bacterium transformed with an expression vector containing a gene encoding a serine-rich foreign protein and an expression vector containing the cysK gene, or a bacterium transformed with an expression vector containing the cysK gene and a gene encoding a serine-rich foreign protein and isolating the foreign protein therefrom. The present invention is expected to be widely used to increase the production yield of a serine-rich foreign protein. | 06-04-2009 |
20090203095 | NOVEL ENGINEERED MICROORGANISM PRODUCING HOMO-SUCCINIC ACID AND METHOD FOR PREPARING SUCCINIC ACID USING THE SAME - The present invention relates to a mutant microorganism, which is selected from the group consisting of genus | 08-13-2009 |
20090215048 | METHOD OF IN-SILICO IMPROVEMENT OF ORGANISMS USING THE FLUX SUM OF METABOLITES - The present invention relates to an in silico method for improving an organism on the basis of the flux sum (φ) of metabolites, and more particularly to a method for screening key metabolites that increase the production yield of a useful substance, the method comprising defining the metabolite utilization of an organism for producing a useful substance as flux sum and perturbing the flux sum, as well as a method for improving an organism producing a useful substance, the method comprising deleting and/or amplifying genes associated with the aforementioned screened key metabolites. According to the present invention, the correlation between specific metabolites and useful substance production can be exactly predicted, so that it is possible to develop an organism having increased useful substance production by introducing and/or amplifying and/or deleting genes expressing enzymes associated with the specific metabolites. In addition, it is also possible to increase the production of a useful substance by adding specific metabolites during culture. | 08-27-2009 |
20090269750 | MARKER AND METHOD FOR CANCER DIAGNOSIS - The present invention relates to a diagnostic cancer marker using variation of a granulocyte colony stimulating factor (G-CSF) gene and a method for preparing the same, and more specifically, relates to a method for diagnosing cancer and/or assessing the state of cancer progression using an oligonucleotide having the 3′-terminal end of exon 2 region linked to the 5′-terminal end of exon 4 region of a G-CSF gene as a diagnostic cancer marker. According to the present invention, cancer can be quickly and exactly diagnosed using variation in a G-CSF gene expression. | 10-29-2009 |
20090298070 | METHOD FOR ANALYZING METABOLITES FLUX USING CONVERGING RATIO DETERMINANT AND SPLIT RATIO DETERMINANT - The present invention relates to a method for analyzing metabolic flux using CRD and SRD. Specifically, the method comprising: selecting a specific target organism, constructing the metabolic network model of the selected organism, identifying the correlations between specific metabolic fluxes in the metabolic network model, defining the correlation ratios as CRD and SRD, determining the correlation ratios of the metabolic fluxes through the experiment for measuring metabolic flux ratios, modifying a stoichiometric matrix with the determined CRD, SRD and correlation ratios, and applying the modified stoichiometric matrix of the metabolic network model for linear programming. According to the inventice method, the correlation between influent/effluent metabolic fluxes with respect to specific metabolites in target organisms (including | 12-03-2009 |
20090298706 | METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM - The present invention relates to a method for expressing a target protein on the surface of a microorganism using | 12-03-2009 |
20090305328 | METHOD OF IMPROVEMENT OF ORGANISMS USING PROFILING THE FLUX SUM OF METABOLITES - The present invention relates to a method for improving an organism through the profiling of flux sum of metabolites, and more particularly to a method for screening key metabolites, the method comprises: plotting a profile between objective functions based on useful substance formation rate as a main function through an algorithm perturbing other functions influencing the production of useful substance; determining the utilization (flux sum (Φ)) of all metabolites from the profile; and screening key metabolites, which show an increase in flux sum (Φ) with an increase in useful substance formation rate. The present invention also relates to a method for improving an organism producing a useful substance, the method comprises introducing and/or amplifying genes associated with the screened key metabolites or introducing the genes from the outside into the organism. According to the disclosed invention, the metabolic utilization (flux sum; Φ) of specific metabolites according to an increase in useful substance formation rate can be predicted, so that key metabolites in increasing the production of a useful substance can be screened. Also, it is possible to increase the production of a useful substance through the method of improving a target organism by introducing and/or amplifying genes associated with the screened metabolites or through the method of supplying the metabolites during the culture of the organism. | 12-10-2009 |
20090305394 | DNA CHIP FOR DIAGNOSIS OF CORNEAL DYSTROPHY - The present invention relates to oligonucleotides for diagnosis of corneal dystrophy. More particularly, the present invention relates to oligonucleotides for detecting mutation of BIGH3 gene for diagnosis or corneal dystrophy including Avellino corneal dystrophy, which must be precisely diagnosed before vision correction surgery, and a DNA chip for diagnosis of corneal dystrophy, which has the oligonucleotides fixed thereon. According to the present invention, conventional microscopic diagnosis of corneal dystrophy can be replaced with a precise genetic method, which prevents a patient with corneal dystrophy from losing eyesight by eyesight correction surgery after erroneous diagnosis. | 12-10-2009 |
20100009432 | CONDUCTIVE CARBON NANOTUBES DOTTED WITH METAL AND METHOD FOR FABRICATING A BIOSENSOR USING THE SAME - Conductive carbon nanotubes (CNTs) obtained by dotting carboxylated CNTs with metal nanocrystals by chemical functional groups, are described, as well as a method for fabricating a pattern or film of the conductive CNTs which involves repeatedly depositing conductive CNTs on a substrate to achieve high surface density. A biosensor is described, in which bioreceptors that bind to target biomolecules are selectively attached to conductive CNTs or a conductive CNT pattern or film. By use of the conductive biosensor, various target biomaterials that bind or react with the bioreceptors can be precisely measured directly or by electrochemical signals at large amounts in one step. Additionally, the biosensor can be used for an electrical detection method capable of providing precise measurement results even with a small amount of source material. | 01-14-2010 |
20100035362 | BIO-SILICA CHIP COMPRISING SILICA BINDING PROTEIN AND METHOD FOR FABRICATING THE SAME - The present invention relates to a bio-silica chip comprising a silica-binding protein and a fabrication method thereof, and more particularly to a bio-silica chip in which a fusion protein of a silica-binding protein and a probe protein is immobilized on a chip comprising a silica layer, a fabrication method thereof and a method of using the bio-silica chip to detect interactions with biomaterials. The bio-silica chip will be very useful in biosensors, etc., because the bio-silica chip is advantageous in that it does not cause non-specific protein binding in the detection of protein-DNA, protein-ligand, protein-antibody, protein-peptide, protein-carbohydrate, protein-protein and cell-biomaterial interactions. Also, in the method for fabricating the bio-silica chip, a probe chip can be selectively immobilized on a silica device chip, which is widely used in biosensors, without a chemical surface treatment process. Thus, a chip fabricating process is simplified and a complicated process for purifying the probe protein becomes unnecessary, thus providing great improvements in productivity and economic efficiency | 02-11-2010 |
20100050298 | MUTANTS OF PHA SYNTHASE FROM PSEUDOMONAS SP. 6-19 AND METHOD FOR PREPARING LACTATE HOMOPOLYMER OR COPOLYMER USING THE SAME - The present invention relates to polyhydroxyalkanoate synthase (PHA synthase) mutant originated from | 02-25-2010 |
20100136640 | ENHANCED BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING BUTANOL USING THE SAME - The present invention relates to a recombinant mutant microorganism having enhanced butanol producing capacity and a method for producing butanol using the same. In the microorganism, genes coding for enzymes responsible for the biosynthesis of lactate, ethanol and/or acetate are deleted or attenuated and genes coding for enzymes involved in butanol biosynthesis are introduced and amplified. | 06-03-2010 |
20100143911 | METHOD FOR SCREENING ESSENTIAL METABOLITES IN GROWTH OF MICROORGANISMS - The present invention disclosed is a method for screening metabolites essential for the growth of microorganism using metabolic flux analysis. More specifically, the present invention relates to the method for screening metabolites essential for the growth of microorganism, by selecting a target microorganism, constructing a metabolic network model of the selected microorganism, inactivating the consumption reaction of each of metabolites in the constructed metabolic network model, analyzing the metabolic flux of the metabolites to select metabolites essential for the growth of the microorganism, and confirming the selected metabolites using the utilization of each of the metabolites, defined as flux sum (Φ). According to the present invention, metabolites essential for the growth of microorganism, and genes involved in the essential metabolites, can be screened in a convenient manner, and drug-target genes against pathogenic microorganisms can be predicted by deleting genes associated with the metabolites screened according to the method. | 06-10-2010 |
20100143985 | METHOD FOR PREPARING BUTANOL THROUGH BUTYRYL-COA AS AN INTERMEDIATE USING YEAST - Disclosed herein are a method for producing butanol in yeast having the ability to biosynthesize butanol using butyryl-CoA as an intermediate, the method comprises producing butyryl-CoA in yeast having a CoAT (acetyl-CoA:butyryl-CoA CoA-transferase)-encoding gene introduced thereinto, through various pathways, and then converting the produced butyryl-CoA to butanol. | 06-10-2010 |
20100159544 | HOMO-SUCCINIC ACID PRODUCING MICROORGANISM VARIANT AND PROCESS FOR PREPARING SUCCINIC ACID USING THE SAME - The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions. | 06-24-2010 |
20100167951 | DNA CHIP FOR DETECTION OF STAPHYLOCOCCUS AUREUS - The present invention relates to nucleic acid probes specific to | 07-01-2010 |
20100167956 | DNA CHIP FOR DETECTION OF ESCHERICHIA COLI - The present invention relates to nucleic acid probes specific to | 07-01-2010 |
20100203599 | MUTANT MICROORGANISMS HAVING A HIGH ABILITY TO PRODUCE PUTRESCINE AND METHOD FOR PRODUCING PUTRESCINE USING THE SAME - Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications. | 08-12-2010 |
20100222545 | COPOLYMER COMPRISING 4-HYDROXYBUTYRATE UNIT AND LACTATE UNIT AND ITS MANUFACTURING METHOD - The present invention relates to a copolymer comprising 4-hydroxybutyrate monomer unit and lactate monomer unit, a copolymer 4-hydroxybutyrate monomer unit, lactate monomer unit and 3-hydroxyalkanoate, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer; 4-hydroxybutyrate monomer; and optionally 3-hydroxyalkanoate, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, phosphotransbutylase gene, butyrate kinase gene and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used for medical use. | 09-02-2010 |
20100286381 | DIAGNOSTIC METHOD FOR CANCER CHARACTERIZED IN THE DETECTION OF THE DELETION OF G-CSF EXON 3 - Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker. | 11-11-2010 |
20100317790 | GRAPHENE COMPOSITE NANOFIBER AND PREPARATION METHOD THEREOF - Disclosed are a graphene composite nanofiber and a preparation method thereof. The graphene composite nanofiber is produced by dispersing graphenes to at least one of a surface and inside of a polymer nanofiber or a carbon nanofiber having a diameter of 1˜1000 nm, and the graphenes include at least one type of monolayer graphenes, and multilayer graphenes having a thickness of 10 nm or less. The graphene composite nanofiber can be applied to various industrial fields, e.g., a light emitting display, a micro resonator, a transistor, a sensor, a transparent electrode, a fuel cell, a solar cell, a secondary cell, and a composite material, owing to a unique structure and property of graphene. | 12-16-2010 |
20100320086 | FLEXIBLE BIOSENSOR AND MANUFACTURING METHOD FOR THE SAME - Provided are a flexible biosensor using a gold binding substance and a method for manufacturing the same. | 12-23-2010 |
20100330634 | Mutants Having Capability To Produce 1, 4-Butanediol And Method For Preparing 1, 4-Butanediol Using The Same - A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | 12-30-2010 |
20110003349 | L-Threonine Overproducing Microorganism and Method for Preparing L-Threonine Using the Same - The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified. | 01-06-2011 |
20110008855 | Recombinant Microorganism Capable Of Producing Polylactate Or Polylactate Copolymer From Sucrose And Method For Producing Polylactate Or Polylactate Copolymer From Sucrose Using The Same - Provided are a microorganism capable of producing a polylactate (PLA) or PLA copolymer from sucrose and a method of producing the PLA or PLA copolymer from sucrose using the same. A gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate are introduced into a microorganism capable of using sucrose as a substrate, and the microorganism is cultured using sucrose as the substrate, thereby allowing efficient production of a PLA or PLA copolymer. | 01-13-2011 |
20110020888 | METHOD FOR PREPARING BUTANOL THROUGH BUTYRYL-CoA AS AN INTERMEDIATE USING BACTERIA - The present invention relates to a method for producing butanol using a bacterium capable of biosynthesizing butanol from butyryl-CoA as an intermediate. More particularly, a method for producing butanol, the method comprising generating bytyryl-CoA in a bacterium which contains a gene coding for AdhE (an enzyme responsible for the conversion of butyryl-CoA to butanol) using various methods, and converting the butyryl-CoA into butanol. | 01-27-2011 |
20110027845 | ENHANCED ETHANOL AND BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING ETHANOL AND BUTANOL USING THE SAME - The present invention relates to a recombinant microorganism having an enhanced ability to produce ethanol and butanol and a method for preparing ethanol and butanol using the same, and more particularly to a recombinant microorganism having an enhanced ability to produce ethanol and butanol, into which a gene encoding CoA transferase and a gene encoding alcohol/aldehyde dehydrogenase are introduced, and to a method for preparing ethanol and butanol using the same. The recombinant microorganism according to the present invention, obtained by manipulating metabolic pathways of microorganisms, is capable of producing butanol and ethanol exclusively without producing any byproduct, and thus is useful as a microorganism producing industrial solvents and transportation fuel. | 02-03-2011 |
20110046339 | COPOLYMER CONTAINING 3-HYDROXYALKANOATE UNIT AND LACTATE UNIT, AND ITS MANUFACTURING METHOD - The present invention relates to a copolymer comprising 3-hydroxyalkanoate monomer unit and lactate monomer unit, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer and 3-hydroxyalkanoate monomer, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used also for medical use. | 02-24-2011 |
20110124131 | METHOD FOR PREPARING METAL NANOPARTICLE USING METAL BINDING PROTEIN - The present invention relates to a method of preparing heavy metal nanoparticles using a heavy metal-binding protein. More specifically, relates to a method for preparing heavy metal structures, comprising the steps of: culturing a microorganism transformed with a gene encoding a heavy metal-binding protein, in a heavy metal ion-containing medium, to produce heavy metal structures in the microorganism; and collecting the produced heavy metal structures, as well as nanoparticles of heavy metal structures prepared according to said method. Unlike prior methods of preparing quantum dots by physically binding metal materials, en the quantum dots disclosed herein can be efficiently produced by expressing the heavy metal-binding protein in cells. In addition, the quantum dots are useful because they can solve an optical stability problem that is the shortcoming of organic fluorophores. | 05-26-2011 |
20110165586 | Detection Method of Bio-Chemical Material Using Surface-Enhanced Raman Scattering - Provided is a detection method of a biochemical material using surface-enhanced Raman scattering in order to detect the existence of a biochemical material in a target subject or its content therein, more particularly a detection method of a biochemical material facilitating multiplex detection with high-sensitivity, high-reproducibility, high-reliability, and high-precision owing to multiple hot spots formed on the nanowire surface of a single crystal body by the bond of multiple nanoparticles which are physically separated from each other. | 07-07-2011 |
20110201067 | METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF - Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful. | 08-18-2011 |
20110244515 | METHOD OF PREPARING PROTEIN HAVING HIGH CONTENT OF SPECIFIC AMINO ACID BY CO-EXPRESSION WITH TRNA OF SPECIFIC AMINO ACID - The present invention relates to a method of increasing the expression of a target protein by co-expression of a gene encoding a target protein having a high content of a specific amino acid with a nucleotide sequence encoding the tRNA of the specific amino acid. According to the present invention, the expression of a protein having a high content of a specific amino acid can be remarkably increased by co-expression with the tRNA of the specific amino acid. Thus, the present invention is useful for increasing the productivity of a protein having a high content of a specific amino acid, such as a repetitive protein. | 10-06-2011 |
20110269183 | RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE - The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose. | 11-03-2011 |
20120118827 | METHOD OF CONCENTRATING LOW TITER FERMENTATION BROTHS USING FORWARD OSMOSIS - The present invention relates to a method for concentrating law titer fermentation broth, and more particularly to a method for concentrating a fermentation broth using forward osmosis. The method comprises: introducing the fermentation broth and an osmolyte into a feed chamber and a draw chamber, respectively, which are included in a concentration system and are divided from each other by a forward osmosis membrane, and subjecting the introduced fermentation broth to forward osmosis, thereby concentrating the fermentation broth in the feed chamber. The method can maximize the concentration of the low titer fermentation broth while minimizing energy consumption and operating cost, and thus can contribute to the industrialization of new technology. | 05-17-2012 |
20120171737 | PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS - Mutants of various polyhydroxyalkanoate (PHA) synthases capable of synthesizing a lactate polymer (PLA) and a lactate copolymer (PLA copolymer), and a method of preparing a lactate polymer and a lactate copolymer using the same are provided. More specifically, a mutant of polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8, and a method of preparing lactate polymer and lactate copolymer using the mutant of synthase are provided. The polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8 can have an activity of synthesizing a lactate polymer and a lactate copolymer by an amino acid sequence mutation affecting an activity of synthesizing a lactate polymer, and can produce a lactate polymer and a copolymer that have different features, respectively, by using the mutants of the synthase. | 07-05-2012 |
20120190088 | METHOD OF PRODUCING FATTY ACID ALKYL ESTER USING MICROORGANISMS HAVING ABILITY TO PRODUCE OIL - The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel. | 07-26-2012 |
20120231499 | HIGH-MOLECULAR-WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN AND MICRO- OR NANO-SIZED SPIDER SILK OR SILK-LIKE FIBER PRODUCED THEREFROM - A high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications. | 09-13-2012 |
20120301936 | RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME - The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol. | 11-29-2012 |
20120322684 | G-CSF PROTEIN HAVING A DELETION OF AN AMINO ACID SEQUENCE CORRESPONDING TO EXON 3 FOR USE AS A DIAGNOSTIC CANCER MARKER - Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker. | 12-20-2012 |
20120329078 | METHOD OF DIAGNOSING CANCER USING G-CSF PROTEIN HAVING A DELETION OF AN AMINO ACID SEQUENCE CORRESPONDING TO EXON 3 AS A DIAGNOSTIC CANCER MARKER - Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker. | 12-27-2012 |
20130017588 | RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAMEAANM Lee; Sang YupAACI DaejeonAACO KRAAGP Lee; Sang Yup Daejeon KRAANM Jang; Yu-SinAACI DaejeonAACO KRAAGP Jang; Yu-Sin Daejeon KRAANM Lee; Jin YoungAACI IncheonAACO KRAAGP Lee; Jin Young Incheon KRAANM Lee; Joung MinAACI Gangwon-doAACO KRAAGP Lee; Joung Min Gangwon-do KRAANM Park; Jin HwanAACI DaejeonAACO KRAAGP Park; Jin Hwan Daejeon KR - The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels. | 01-17-2013 |
20130078673 | RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE - The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose. | 03-28-2013 |
20130130344 | MICROORGANISM VARIANTS HAVING HYDROCARBON PRODUCING ABILITY AND METHOD FOR PRODUCING HYDROCARBON USING THE SAME - The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane. | 05-23-2013 |
20130157323 | MUTANT MICROORGANISM HAVING HIGH PRODUCTION OF CADAVERINE, AND PREPARATION METHOD OF CADAVERINE USING SAME - The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications. | 06-20-2013 |
20130164805 | Recombinant Microorganisms Having Enhanced Propanol and Method for Preparing Propanol Using the Same - The present invention relates to mutant microorganisms having the ability to produce propanol in high concentration and high yield, and to a method of producing propanol using the same. More particularly, the invention relates to mutant microorganisms having the ability to produce propanol in high concentration and high yield, which have introduced therein genes that encodes enzymes which are involved in the biosynthesis of propanol from threonine, and to a method of producing propanol using the same | 06-27-2013 |
20130217087 | NOVEL MUTANT MICROORGANISM PRODUCING SUCCINIC ACID SIMULTANEOUSLY USING SUCROSE AND GLYCEROL, AND METHOD FOR PREPARING SUCCINIC ACID USING SAME - The present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously as carbon sources. More particularly, the present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously for succinic acid production, the mutant organism being obtained by relieving the mechanism of sucrose-mediated catabolite repression of glycerol in a succinic acid-producing microorganism. | 08-22-2013 |
20140005365 | ANTIBODY TO A MUTANT G-CSF FOR DIAGNOSIS OF CANCER | 01-02-2014 |
20140030775 | RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE] COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE] COPOLYMER USING SAME - There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment. | 01-30-2014 |
20140093901 | GENOME-SCALE METABOLIC NETWORK MODEL RECONSTRUCTION OF KLUYVEROMYCES MARXIANUS AND STRATEGIES FOR ENGINEERING NON-NATIVE PATHWAYS FOR 3-HYDROXYPROPIONATE PRODUCTION IN KLUYVEROMYCES MARXIANUS - Use of a metabolic network model for analyzing metabolic characteristics of microorganisms for producing a metabolic product, such as 3HP enabling estimation of productivity and cell growth speed of microorganisms, optimizing new metabolic pathway, and providing transformed microorganisms that may produce a specific metabolic product with high efficiency. | 04-03-2014 |
20140243222 | MULTI-SPOT METAL-CAPPED NANOSTRUCTURE ARRAY NUCLEIC ACID CHIP FOR DIAGNOSING OF CORNEAL DYSTROPHY AND PREPARATION METHOD THEREOF PRODUCING SAME - A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. The metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases. | 08-28-2014 |
20140377752 | NOVEL SYNTHESIS-REGULATING SRNA AND METHOD FOR PREPARING SAME - The present invention relates to a novel customized sRNA that reduces gene expression in prokaryotic cells, a preparation method thereof, and the use thereof, and more particularly to a synthetic sRNA comprising an Hfq binding site, derived from the sRNA of any one of MicC, SgrS and MicF, and a region that base-pairs with the target gene mRNA, and to a preparation method thereof and the use thereof. The synthetic sRNA according to the invention has an advantage in that the degree of inhibition of the target gene can be controlled by regulating the ability of the synthetic sRNA to bind to the mRNA of the target gene. The use of the synthetic sRNA that regulates the expression of the target gene makes it possible to effectively construct a recombinant microorganism without using a conventional gene deletion method and to reduce the expression of the target gene, and thus the synthetic sRNA is useful for the production of recombinant microorganisms. Also, the synthetic sRNA can be quickly applied to various strains, and thus is very suitable for the measurement of metabolic capabilities of strains and the selection of the most suitable strain. In addition, recombinant microorganisms, which are obtained by metabolic flux manipulation using the synthetic sRNA and produce tyrosine or cadaverine with high efficiency, are useful in the drug and industrial fields. In other words, the use of the sRNA according to the present invention can make it easy to select target genes whose expression is to be inhibited for the highly efficient production of metabolites. Accordingly, the synthetic sRNA can be used to construct recombinant strains for efficient production of various metabolites and to establish efficient methods for production of various metabolites, and thus is highly useful. | 12-25-2014 |