Patent application number | Description | Published |
20090118128 | Preparation of templates for nucleic acid sequencing - The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise:
| 05-07-2009 |
20090176662 | End Modification to Prevent Over-Representation of Fragments - The invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides and also the use of the 5′ and 3′ modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from whence the 5′ and 3′ modified library is generated is greatly reduced or prevented. | 07-09-2009 |
20110009276 | Method for Sequencing a Polynucleotide Template - The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. | 01-13-2011 |
20110223601 | Method for pairwise sequencing of target polynucleotides - The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template. | 09-15-2011 |
20120094848 | Method for pairwise sequencing of target polynucleotides - The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template. | 04-19-2012 |
20120122737 | METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES - The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction. | 05-17-2012 |
20120172259 | USING POPULATIONS OF BEADS FOR THE FABRICATION OF ARRAYS ON SURFACES - The present invention provides a method of creating an array of features. The method can include steps of (a) providing a plurality of beads, wherein each bead in the plurality of beads includes probe content; (b) contacting the plurality of beads with a surface to produce a layer of beads on the surface; and (c) transferring the probe content from the beads to the surface to create an array of spatially discrete features on the surface, wherein each spatially discrete feature includes probe content from a bead in the plurality of beads. | 07-05-2012 |
20130244886 | COMPOSITIONS AND METHODS FOR SEQUENCING NUCLEIC ACIDS - Disclosed herein are compositions and methods for sequencing nucleic acids. | 09-19-2013 |
20130274115 | METHOD FOR PAIRWISE SEQUENCING OF TARGET POLYNUCLEOTIDES - The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template. | 10-17-2013 |
20130281306 | SEQUENCING METHODS - The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to nucleic acid sequencing. | 10-24-2013 |
20140024537 | METHODS AND SYSTEMS FOR DETERMINING HAPLOTYPES AND PHASING OF HAPLOTYPES - The present disclosure provides methods and systems for determining and/or characterizing one or more haplotypes and/or phasing of haplotypes in a nucleic acid sample. In particular, the disclosure provides methods for determining a haplotype and/or phasing of haplotypes in a nucleic acid sample by incorporating synthetic polymorphisms into fragments of a nucleic acid sample and utilizing the synthetic polymorphisms in determining one or more haplotypes and/or phasing of haplotypes. | 01-23-2014 |
20140080721 | METHODS FOR REDUCING NUCLEIC ACID DAMAGE - Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample. | 03-20-2014 |
20140287415 | Polymerases - Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays. | 09-25-2014 |
20140287935 | COMPOSITIONS AND METHODS FOR SEQUENCING NUCLEIC ACIDS - Disclosed herein are compositions and methods for sequencing nucleic acids. | 09-25-2014 |
20140336057 | METHOD FOR PAIRWISE SEQUENCING OF TARGET POLYNUCLEOTIDES - The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template. | 11-13-2014 |