Patent application number | Description | Published |
20100291570 | MATERIALS AND METHODS FOR DETECTION OF NUCLEIC ACIDS - Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample. | 11-18-2010 |
20120295808 | Solid Support Assay Systems and Methods Utilizing Non-Standard Bases - Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay. | 11-22-2012 |
20140024550 | Solid Support Assay Systems and Methods Utilizing Non-Standard Bases - Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay. | 01-23-2014 |
20140057269 | Materials and Methods for Detection of Nucleic Acids - Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample. | 02-27-2014 |
Patent application number | Description | Published |
20090203540 | Methods and Systems for Quality Control Metrics in Hybridization Assays - The present invention provides methods and systems for performing quality control metrics in hybridization assays. In particular, the present invention provides for quality control metrics for nucleic acid enrichment on hybridization assay formats, such as microarray assays. | 08-13-2009 |
20090221438 | METHODS AND SYSTEMS FOR UNIFORM ENRICHMENT OF GENOMIC REGIONS - The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules. | 09-03-2009 |
20100222232 | ENRICHMENT AND SEQUENCE ANALYSIS OF GENOMIC REGIONS - The present invention provides novel methods for reducing the complexity of preferably a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented (genomic) nucleic acids for reducing the genetic complexity of the original population of nucleic acid molecules. | 09-02-2010 |
20100331204 | METHODS AND SYSTEMS FOR ENRICHMENT OF TARGET GENOMIC SEQUENCES - The present invention provides methods and systems for targeted nucleic acid sequence enrichment in a sample. In particular, the present invention provides for enriching for targeted nucleic acid sequences during hybridizations in hybridization assays by first depleting non-target nucleic acid sequences. | 12-30-2010 |
20110153222 | OPTIMIZED PROBE SELECTION METHOD - The present invention provides methods for optimizing oligonucleotide hybridization probes for use in basic and clinical research. Specifically, the invention involves hybridizing serially diluted genomic sample to the oligonucleotide probes on the array, such that a signal intensity is produced for each of the probes; computationally identifying optimized probes which exhibit signal intensities that correspond to the serial dilutions of genomic sample and are reproducibly strong relative to non-optimized probes. | 06-23-2011 |
20120071357 | METHODS AND SYSTEMS FOR UNIFORM ENRICHMENT OF GENOMIC REGIONS - The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules. | 03-22-2012 |
20120088686 | METHODS OF ARRAY DATA WAVE CORRECTION - The invention relates to methods of correcting auto-correlated wave artifacts of array signal data based on the GC content of the nucleic acids under consideration without affecting signal variations due to copy number variations. | 04-12-2012 |
20120238477 | METHODS FOR SYNTHESIS OF AN OLIGOPEPTIDE MICROARRAY - An oligopeptide microarray and methods for the synthesis thereof are presented. Further presented is a microarray on a solid support comprising at least about 10,000 oligopeptide features per cm | 09-20-2012 |
20120245057 | WHOLE PROTEOME TILING MICROARRAYS - The present invention relates to a microarray comprising at least 50,000 oligopeptide features per cm | 09-27-2012 |
20140031244 | OPTIMIZED PROBE SELECTION METHOD - The present invention provides methods for optimizing oligonucleotide hybridization probes for use in basic and clinical research. Specifically, the invention involves hybridizing serially diluted genomic sample to the oligonucleotide probes on the array, such that a signal intensity is produced for each of the probes; computationally identifying optimized probes which exhibit signal intensities that correspond to the serial dilutions of genomic sample and are reproducibly strong relative to non-optimized probes. | 01-30-2014 |