Patent application number | Description | Published |
20090189089 | VALVE CONTROL SYSTEM FOR A ROTATING MULTIPLEX FLUORESCENCE DETECTION DEVICE - Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser. | 07-30-2009 |
20090218517 | MULTIPLEX FLUORESCENCE DETECTION DEVICE HAVING REMOVABLE OPTICAL MODULES - Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules. Each of the removable optical modules is optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. | 09-03-2009 |
20110039274 | ANALYSIS OF NUCLEIC ACID AMPLIFICATION CURVES USING WAVELET TRANSFORMATION - A method comprising acquiring amplification data proportional to an amount of nucleic acid present for each of a plurality of PCR cycles includes applying wavelet transformation to the amplification data to determine a PCR cycle corresponding to a point within a growth period of the amplification data, and updating a display A device including a control module, an analysis module and an interface module for initialization of PCR analysis of a nucleic acid sample, receiving amplification data proportional to an amount of nucleic acid present applying wavelet transformation to the amplification data to determine a PCR cycle corresponding to a point within a growth period of the amplification data, and updating a display based on the amplification data is also provided. | 02-17-2011 |
20110117607 | ANNULAR COMPRESSION SYSTEMS AND METHODS FOR SAMPLE PROCESSING DEVICES - Systems and methods for processing sample processing devices. The system can include a base plate adapted to rotate about a rotation axis. The base plate can include at least one first magnetic element. The system can further include an annular cover, and a sample processing device comprising at least one thermal process chamber. The annular cover can include an inner edge, an outer edge, and at least one second magnetic element. The method can include positioning the sample processing device between the base plate and the annular cover, such that the inner edge of the annular cover is positioned inwardly of the at least one thermal process chamber, and such that the at least one first magnetic element attracts the at least one second magnetic element to force the annular cover in a first direction along the z-axis, urging the sample processing device into contact with the base plate. | 05-19-2011 |
20110117656 | SYSTEMS AND METHODS FOR PROCESSING SAMPLE PROCESSING DEVICES - A system and method for processing sample processing devices. The system can include a base plate adapted to rotate about a rotation axis. The system can further include a cover including a first projection, and a housing. A portion of the housing can be movable with respect to the base plate between an open position and a closed position, and can include a second projection. The first projection and the second projection can be adapted to be coupled together when the portion is in the open position and decoupled when the portion is in the closed position. The method can include coupling the cover to the portion of the housing, moving the portion of the housing from the open position to the closed position, and rotating the base plate about the rotation axis. | 05-19-2011 |
20110182770 | BIOLOGICAL STERILIZATION INDICATOR, SYSTEM, AND METHODS OF USING SAME - A biological sterilization indicator, system, and methods of determining the effectiveness of a sterilization process. The biological sterilization indicator can include a locus of spores, a reservoir containing a liquid, and a sterilant path positioned to provide fluid communication between ambience and the locus of spores. The reservoir can have a closed state in which the reservoir is not in fluid communication with the locus of spores and an open state in which the reservoir is in fluid communication with the locus of spores. The biological sterilization indicator system can include the biological sterilization indicator and a detection device adapted to be coupled to the biological sterilization indicator. In some embodiments, the method can include assaying the spores for a detectable change in a characteristic, and detecting substantially all of the detectable change. | 07-28-2011 |
20120171677 | COMPENSATION FOR SPECTRAL CROSSTALK IN MULTIPLEX NUCLEIC ACID AMPLIFICATION - A method includes performing a nucleic acid amplification of a nucleic acid sample using a detection probe, wherein the nucleic acid amplification occurs over one or more interrogation periods, and, from the nucleic acid amplification, acquiring amplification data that indicates an amount of nucleic acid present for each of the one or more interrogation periods. The method also includes, based on the amplification data, determining a crosstalk correction value associated with a spectral neighbor to the probe to reduce spectral crosstalk from the spectral neighbor; and applying the crosstalk correction value to amplification data collected from multiplex nucleic acid amplifications of nucleic acid samples. | 07-05-2012 |
20120291538 | SYSTEMS AND METHODS FOR VOLUMETRIC METERING ON A SAMPLE PROCESSING DEVICE - A system and method for volumetric metering on a sample processing device. The system can include a metering reservoir, and a waste reservoir positioned in fluid communication with a first end of the metering reservoir to catch excess liquid from the metering reservoir that exceeds a selected volume. The system can further include a capillary valve in fluid communication with the second end of the metering reservoir to inhibit liquid from exiting the metering reservoir until desired. The method can include metering the liquid by rotating the sample processing device to exert a first force on the liquid that is insufficient to move the liquid into the capillary valve, and rotating the sample processing device to exert a second force on the liquid that is greater than the first force to move the metered volume of the liquid to the process chamber via the capillary valve. | 11-22-2012 |
20120291565 | SYSTEMS AND METHODS FOR VALVING ON A SAMPLE PROCESSING DEVICE - A system and method for valving on a sample processing device. The system can include a valve chamber, a process chamber, and a valve septum located between the valve chamber and the process chamber. The system can further include a fluid pathway in fluid communication with an inlet of the valve chamber, wherein the fluid pathway is configured to inhibit a liquid from entering the valve chamber and collecting adjacent the valve septum when the valve septum is in a closed configuration. The method can include rotating the sample processing device to exert a first force on the liquid that is insufficient to move the liquid into the valve chamber; forming an opening in the valve septum; and rotating the sample processing device to exert a second force on the liquid to move the liquid into the valve chamber. | 11-22-2012 |
20120293796 | SYSTEMS AND METHODS FOR DETECTING THE PRESENCE OF A SELECTED VOLUME OF MATERIAL IN A SAMPLE PROCESSING DEVICE - Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material. | 11-22-2012 |