Patent application number | Description | Published |
20140118775 | Automated Printer's Dashboard - Techniques are provided for generating and using automated production and performance dashboards. For example, an automated production dashboard is generated and used that includes, but is not limited to, defining production performance metrics, job stats, trends, red/green/yellow with health check, and risks. As well, an automated upkeep dashboard is generated and used that includes, but is not limited to, defining supplies, maintenance, trends, next service appointments, red/green/yellow with health check, and risks. Such techniques may include a remote view of such dashboards, e.g. via native mobile applications in addition to web/client, etc. | 05-01-2014 |
Patent application number | Description | Published |
20100129879 | GENOME PARTITIONING USING A NICKING ENDONUCLEASE - A method for partitioning a genome is provided. In certain embodiments, the method comprises: a) nicking a region of the genome using a sequence-specific nicking endonuclease to produce a nicked double-stranded genomic region; b) hybridizing the nicked double-stranded genomic region with an oligonucleotide comprising: i. an affinity tag; and ii. a nucleotide sequence that is complementary to the nucleotide sequence that is immediately adjacent to the nick site, to produce a duplex in which a terminal nucleotide of the oligonucleotide lies immediately adjacent to said a nucleotide of the nick site; c) ligating the terminal nucleotide of the oligonucleotide to the nucleotide of the nick site to produce a ligation product; and d) separating the ligation product from unligated products using the affinity tag. Compositions and kits for practicing the method are provided. | 05-27-2010 |
20100137154 | GENOME ANALYSIS USING A METHYLTRANSFERASE - A method of genome analysis is provided. In certain embodiments, the method may comprise: labeling the test genome using a first site-specific methyltransferase to produce a labeled test genome comprising a label; and analyzing the labeled test genome to determine if the test genome comprises a sequence alteration relative to a reference sequence. In certain embodiments, the method may comprise: evaluating binding of the labeled test genome to an array of probes, or observing a pattern of labeling along the labeled test genome. | 06-03-2010 |
20110250595 | ANALYSIS OF SINGLE NUCLEOTIDE POLYMORPHISMS USING END LABELING - A method for analyzing a sequence comprising a SNP site is provide. In general terms, the method comprises: a) contacting a first DNA sample with a first restriction enzyme to provide DNA fragments, wherein: i) the first restriction enzyme cleaves the sequence only if a first allele of a SNP is present at the SNP site; b) end-labeling the DNA fragments to produce an end-labeled sample; c) hybridizing the end-labeled sample to an array comprising a probe sequence; and d) comparing the amount of hybridization between the digested sample and the probe sequence to a reference signal | 10-13-2011 |
Patent application number | Description | Published |
20090036664 | Complex oligonucleotide primer mix - The invention generally relates to a complex mixture of oligonucleotide primers and/or probes. Another aspect of the invention includes a method of selective priming of a target nucleic acid. | 02-05-2009 |
20100120631 | Methods for detecting hepatocellular carcinoma - A method for evaluating hepatocellular carcinoma in a subject is provided. In certain embodiments, the method comprises: a) obtaining a hepatocellular carcinoma protein marker profile for a sample obtained from the subject; and b) comparing the protein marker profile to a control profile. | 05-13-2010 |
20100330556 | GENOME ANALYSIS USING A NICKING ENDONUCLEASE - A method of genome analysis is provided. In certain embodiments, the method of comprises: a) contacting a genomic sample comprising a double-stranded DNA with a site-specific nicking endonuclease to provide a nicked double-stranded DNA comprising a plurality of nick sites, in which the nicking endonuclease nicks a site adjacent to a variable nucleotide; b) contacting the nicked double-stranded DNA with a polymerase in the presence of a nucleotide composition comprising a first labeled nucleotide comprising a first label, thereby producing a labeled double-stranded DNA that is not labeled at every nick site; c) stretching out the labeled double-stranded DNA to provide a stretched, labeled double-stranded DNA; and d) imaging the stretched, labeled double-stranded DNA to identify a labeling pattern on the stretched labeled double-stranded DNA. | 12-30-2010 |
20100330557 | GENOMIC COORDINATE SYSTEM - A method of sample analysis is provided. In certain embodiments, the method comprises: a) site-specifically labeling a test genome with at least two different labels to produce a labeled genome labeled at a plurality of discrete sites across the genome; b) stretching a nucleic acid of the labeled genome to produce a linear pattern of the different labels along a region of a stretched nucleic acid; c) reading the labels along the region to provide a test pattern comprising a sequence of colors emitted by the labels; d) comparing the test pattern to a plurality of reference patterns obtained from a reference genome, in which the reference patterns are mapped to corresponding genomic locations in the reference genome; and e) identifying one or more reference patterns that match the test pattern, thereby mapping a location for the region in the test genome. | 12-30-2010 |
20110039716 | Analysis of Single Nucleotide Polymorphisms Using a Nicking Endonuclease - A method of genome analysis is provided. In certain embodiments, the method comprises: a) contacting a double-stranded genomic DNA with a site-specific nicking endonuclease that recognizes a sequence comprising a single nucleotide polymorphism (SNP), in which the endonuclease nicks the genomic DNA at a nick site only if a first allele of the SNP is present; b) denaturing the genomic sample; c) contacting the denatured genomic sample with an array comprising a first probe and a second probe, in which nicking results in less binding of the denatured sample to the first probe relative to a sample that is not nicked; and d) comparing the amount of hybridization to the first probe to the amount of hybridization to said second probe, in which decreased binding of the denatured genomic samples to the first probe relative to the second probe indicates that the first allele of the SNP is present. | 02-17-2011 |
20140356867 | NUCLEIC ACID ENRICHMENT USING CAS9 - A method of enriching for a fragment of a genome, as well as corresponding compositions and kits, are provided. In certain embodiments, the method comprises: (a) contacting a sample comprising fragmented DNA with a Cas9-gRNA complex comprising mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in the DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented DNA; and (b) isolating the complex. In addition, other methods and compositions for Cas9/CRISPR-mediated nucleic acid manipulation are also provided. | 12-04-2014 |
20140357523 | METHOD FOR FRAGMENTING GENOMIC DNA USING CAS9 - A method for fragmenting a genome is provided. In certain embodiments, the method comprises: (a) combining a genomic sample containing genomic DNA with a plurality of Cas9-gRNA complexes, wherein the Cas9-gRNA complexes comprise a Cas9 protein and a set of at least 10 Cas9-associated guide RNAs that are complementary to different, pre-defined, sites in a genome, to produce a reaction mixture; and (b) incubating the reaction mixture to produce at least 5 fragments of the genomic DNA. Also provided is a composition comprising at least 100 Cas9-associated guide RNAs that are each complementary to a different, pre-defined, site in a genome. Kits for performing the method are also provided. In addition, other methods, compositions and kits for manipulating nucleic acids are also provided. | 12-04-2014 |
20150068901 | Nanofluidic Device for Charge Analysis of Straightened Molecules - This disclosure provides, among other things, a nanofluidic device sensing device is provided. In certain embodiments, the device contains: a) a channel comprising a floor and a ceiling, b) an array of charge sensors in the floor and/or ceiling of the channel, arranged along the longitudinal axis of the channel; c) a capture area in the floor and/or ceiling of the channel at the entrance end of the channel; and d) a first electrode and a second electrode, wherein the first and second electrodes are positioned to provide an electrophoretic force along the longitudinal axis of the channel. Other embodiments, e.g., methods, are also described. | 03-12-2015 |
20150132756 | POLYMERASE IDLING METHOD FOR SINGLE MOLECULE DNA SEQUENCING - A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3′ end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a nuclease-resistant nucleotide to the end of the primer after the polymerase has idled on and removed the added chain terminator nucleotide, thereby producing a duplex comprising the template and an extended primer that has a nuclease resistant 3′ end. | 05-14-2015 |
20150148239 | SPATIAL MOLECULAR BARCODING OF IN SITU NUCLEIC ACIDS - This disclosure provides, among other things, a method for analyzing a planar cellular sample. In some embodiments, the method comprises: (a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the feature in which the oligonucleotides is present; (c) hybridizing the nucleic acid tags, or a copy of the same, with the oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in the duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. Other embodiments, e.g., kits and the like, are also described. | 05-28-2015 |
Patent application number | Description | Published |
20130205033 | SESSION INFORMATION TRANSPARENCY CONTROL - Transparency control for session information for a communication network node can be set on a dynamic and/or static basis in relation to properties of a peer, an interface, a trust level, a session or a message. The transparency control can be based on message-related parameters, with the transparency of certain message parts being controlled on a static and/or dynamic basis in relation to transparency control settings. The transparency control can change upon the occurrence of a rerouting event that implicates recalculated transparency control settings. A message can be marked with transparency type settings that are evaluated to determine transparency control for the message. The transparency control can be based on transparency types of topology, dialog, identity, header, body, media, functional or accounting. The transparency control settings can be user selectable to permit transparency behavior tailored to the desires of an operator of the communication network node. | 08-08-2013 |
20150221007 | PERPETUAL COMMUNICATION SESSION: PORTABILITY / REUSABILITY ACROSS APPLICATIONS, NETWORKS AND DEVICES - A System with mechanisms that allows for creating, storing and resuming a session without being bound by time is proposed here. In a system that allows for communication between parties when one side of the party has an account with the system whereas others who are trying to reach this party do not, creating a session and allowing for the same users to come back to resume the session is proposed. Resuming the Session by any of the associated users to the session is supported. This session can be operated through various applications such as voice, video and chat and be accessed in various networks such as the interne and telephone network as well as across devices such as web browser, smart device apps and mobile phones with SMS capability. The system proposed supports porting and reusability of the session across networks, applications and devices. | 08-06-2015 |
20150339745 | INSTANT GENERATION AND USAGE OF HTTP URL BASED UNIQUE IDENTITY FOR ENGAGING IN MULTI-MODAL REAL-TIME INTERACTIONS IN ONLINE MARKETPLACES, SOCIAL NETWORKS AND OTHER RELEVANT PLACES - A System with mechanisms is provided that allows anyone or any entity to signup with basic identity information along with any service description resulting in an instant HTTP URL as a means for outside parties to contact this provider of the service and engage in multi-modal interactions involving voice, video, chat and media sharing. A single user or a group of users can signup with this system and provide optionally some contact information such as phone numbers. A HTTP URL is instantly generated that can be advertised as hyperlinks directly by the users to the outside environment such as on a website, email or any other means. The providers, who have signed-up for the service, can also remain signed-in into the system so as to engage with users wishing to contact them via the published URL. Any user with an internet connection upon clicking this URL will be directed to this proposed system and will be provided with a conversation window through which they can start engaging with the provider via chat, voice and video (webcam) and start sharing with each other any media information such as pictures and videos. The provider if not signed-in into the system can also engage with the user via SMS and email. The HTTP URL is being used as a starting point for users to engage with the providers of any service behind that URL. This URL can be advertised by the proposed system in various online marketplaces or social networks of the provider as well as on the web to be identified by search engines. | 11-26-2015 |
Patent application number | Description | Published |
20130143295 | AMYLASES AND GLUCOAMYLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing. | 06-06-2013 |
Patent application number | Description | Published |
20140121629 | Elastic Introducer Sheath - An elastic percutaneous elastic introducer sheath is disclosed which can locally expand and reduce to accommodate a transcatheter medical device. The elastic introducer sheath includes a non-circumferentially continuous wire structure, a liner, and a jacket. | 05-01-2014 |
20140148889 | Distal Tip for a Delivery Catheter - Distal tips for use with delivery catheters are disclosed that are configured to maintain complete engagement between the distal tip and a distal opening of a sheath component of the delivery catheter so as to prevent separation therebetween and/or to prevent fish-mouthing of a distal leading edge of the sheath component during in vivo use. Distal tips so configured realize one or more of the objectives of safer tracking of the delivery catheter through the vasculature, safe crossing of the delivery catheter through structural components of the vasculature and heart, such as through native valves, and safe removal of the delivery catheter post deployment. | 05-29-2014 |
20140277403 | Devices and Methods for Preparing A Transcatheter Heart Valve System - Devices and methods for preparing a transcatheter heart valve system. The device includes a housing, sealing apparatuses and a port. The housing forms a chamber sized to receive a prosthetic valve and a portion of a delivery device. During use, the prosthesis is seated in the chamber, and the sealing apparatuses operated to seal the chamber about the delivery device. Air bubbles in a liquid delivered through the delivery device and to the chamber are removed from the system via the port, thereby flushing the system. Further, the device is manipulated to permit loading of the prosthesis into the delivery device in an air bubble-free environment. | 09-18-2014 |