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Paul Lem, Ottawa CA

Paul Lem, Ottawa CA

Patent application numberDescriptionPublished
20080275229Method for increasing the speed of nucleic acid amplification reactions - The speed of a nucleic acid amplification reaction, such as the Polymerase Chain Reaction (PCR), can be increased by setting the temperature of heat sources above and below the desired denaturation, annealing, and extension temperatures. The reaction mixture is only contacted with the heat sources long enough for the desired temperatures to be reached.11-06-2008
20090037268Relevance Engine for Delivering Increasingly Relevant Content to Users - An electronic means for delivering increasingly relevant advertising content to users comprises a relevance engine a user enrollment portal so that the user can submit relevant user preference information to the engine, means for determining relevant advertising content to be delivered to the user based on submitted user preferences and use preferences learned by the engine, and, means for adjusting the relevance of advertising content delivered to the user based on user responses to the ads delivered.02-05-2009
20090123976Compositions and Method for Storage of Nucleic Acid From Bodily Fluids - An aqueous composition comprising a denaturing agent, a chelator, a buffering agent and a protease for the extraction of nucleic acid from a sample of bodily fluid, such as saliva, such that the extracted nucleic acid is stable for at least fourteen days at room temperature and can be directly utilised in an amplification reaction without further processing. In particular, said composition comprises SDS, Cyclohexanediamine tetraacetate, Tris-HCl and proteinase K. A method and kit for the amplification of DNA directly from a bodily fluid, comprising said composition, is further provided.05-14-2009
20090216213CONTAINER SYSTEM FOR RELEASABLY STORING A SUBSTANCE - The present invention provides a container system for releasably storing a substance. The container system includes a vial having a sample storage chamber and a piercing member for piercing a membrane in the lid, which membrane seals a substance within a reservoir in the lid until the membrane is pierced by the piercing member. The container system optionally includes a funnel. There is also provided a method and kit for use of such a container system.08-27-2009
20100075296Thermal cycling by positioning relative to fixed-temperature heat source - A thermal cycling system and method are provided herein, The thermal cycling system for performing a biological reaction at two or more different temperatures comprises: a) a heat source for setting at a fixed temperature; b) a reaction vessel containing material upon which the biological reaction is to be performed; c) mechanically-operable means for altering the relative position of the heat source and the reaction vessel so that reaction vessel first achieves and maintains a desired first temperature in the reaction vessel for starting the carrying out of the biological reaction, and then for altering the relative position of the heat source and the reaction vessel so that reaction vessel then achieves and maintains a second temperate for continuing the carrying out of the biological reaction on the biological material, and d) temperature-sensing means operatively associated with the reaction vessel for controlling the altering of the relative position of the heat source and the reaction vessel so that the reaction vessel achieves and maintains the desired second temperature in the reaction vessel.03-25-2010
20100264155TUBE FOR DNA REACTIONS - A reaction tube for use in performing the Polymerase Chain Reaction (PCR) in a thermal cycler, is provided herein such reaction tube includes a hollow tube of heat conducting synthetic plastics material having an open top and a flat bottom formed of an optically-clear material. Such reaction tube also includes a solid plug cap nesting within said hollow tube and to provide a minimized void volume within the hollow tube and to minimize evaporation and condensation of the biological fluid.10-21-2010
20100273218COMPOSITIONS AND METHOD FOR STORAGE OF NUCLEIC ACID FROM BODILY FLUIDS - The present invention provides an aqueous composition comprising SDS, Cyclohexanediamine tetraacetate, Tris-HCl and proteinase K for the extraction of nucleic acid from a sample of bodily fluid, such a saliva, wherein the extracted nucleic acid is stable for at least fourteen days at room temperature The composition permits direct use of the extracted and stored DNA in an amplification reaction without further processing.10-28-2010
20110014658COMPOSITIONS AND METHOD FOR STORAGE OF NUCLEIC ACID FROM BODILY FLUIDS - The present invention provides an aqueous composition and method for extracting nucleic acid from a sample of bodily fluid, such as saliva, such that the nucleic acid within said sample remains stable for at least fourteen days at room temperature. The composition permits direct use of the extracted and stored DNA in an amplification reaction without further processing.01-20-2011
20110035503System and Method for Anonymous Addressing of Content on Network Peers and for Private Peer-to-Peer File Sharing - A system and method for efficient and private peer-to-peer file sharing consists of ascribing a uniquely identified and anonymous link (an “edgelink”) to any file or set of files on a peer computer. The link is registered with a publishing server along with continuously updated connectivity information about the peer without registering any identifying information about the file. A peer recipient is able to access the link, receive connectivity information about the publishing peer from the server, and then receive the file from the publishing peer without file content passing through the server, mediating any intermediary NAT devices without requiring any manual or automatic device reconfiguration.02-10-2011

Patent applications by Paul Lem, Ottawa CA