Patent application number | Description | Published |
20080199930 | METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER - A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC. | 08-21-2008 |
20080306249 | METHOD OF SELECTIVELY REMOVING PROTEIN FROM BIOLOGICAL SAMPLES USING CHEMICALS - Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: | 12-11-2008 |
20090048437 | METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT - The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less. | 02-19-2009 |
20090131649 | METHOD OF SEPARATING SMALL RNA MOLECULES USING KOSMOTROPIC SALT - Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations. | 05-21-2009 |
20090142798 | METHOD OF SELECTIVELY LYSING NON-VIABLE CELLS IN CELL POPULATION IN SAMPLE - The present invention provides a method of selectively lysing non-viable cells from a cell population within a sample, the method including selectively lysing non-viable cells by mixing a cell-containing sample with a lysis buffer including a non-ionic surfactant and a divalent cation salt. | 06-04-2009 |
20090163382 | PRIMER SET FOR AMPLIFYING TARGET SEQUENCE(S) OF ANTIBIOTIC-RESISTANT BACTERIAL SPECIES, PROBE OR PROBE SET SPECIFICALLY HYBRIDIZING WITH TARGET SEQUENCE(S) OF ANTIBIOTIC-RESISTANT BACTERIAL SPECIES, METHOD OF DETECTING ANTIBIOTIC-RESISTANT BACTERIAL SPECIES USING THE PROBE OR PROBE SET, AND KIT FOR DETECTING ANTIBIOTIC-RESISTANT BACTERIAL SPECIES - Provided are a primer set for amplifying target sequence(s) of antibiotic-resistant bacterial species, a probe or probe set specifically hybridizing with target sequence(s) of antibiotic-resistant bacterial species, a microarray immobilized with the probe or probe set, a kit comprising the primer set and a method of detecting at least one antibiotic-resistant bacterial species using the probe or probe set. | 06-25-2009 |
20100190651 | METHOD OF ANALYZING PROBE NUCLEIC ACID, MICROARRAY AND KIT FOR THE SAME - Provided are a method of analyzing a sequence of a first probe nucleic acid using a substrate on which a second probe nucleic acid is immobilized, and a microarray and a kit for the same. | 07-29-2010 |
20110190147 | MICROARRAY REACTION DEVICE AND METHOD OF USING THE SAME - A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber. | 08-04-2011 |
20110237460 | MICROARRAY PACKAGE DEVICE AND METHOD OF MANUFACTURING THE SAME - A microarray package device and a method of manufacturing the same. An effective microarray analyzing reaction is performed by using the microarray package device that provides structural stability and reliable experimental results. | 09-29-2011 |
20130078658 | METHOD OF QUANTIFYING RECOVERY RATE OF EXOSOME - A recombinant exosome comprising a fusion protein of a membrane protein and light-emitting protein, and a method of determining an exosome recovery rate by using the recombinant exosome are provided. Use of the method ensures accurate quantification of exosomes in a sample, and thus, improves the efficiency of an exosome-based diagnosis. | 03-28-2013 |
20130157300 | METHODS OF QUANTIFYING EXOSOME IN CELL CULTURE AND METHOD OF INCREASING RECOVERY RATE OF EXOSOME USING THE SAME - A method of detecting and recovering of exosomes in a sample, as well as a method of determining the recovery rate of exosomal recovery, and a method of screening for a material that induces secretion of the exosomes in a sample, which methods employ the use of a protease. | 06-20-2013 |
20130280713 | POLYNUCLEOTIDE AND USE THEREOF - A first polynucleotide including at least two complementary regions that are complementary to a target nucleic acid and have a reverse configuration, a second polynucleotide complementary to the first polynucleotide, and uses thereof, are provided. | 10-24-2013 |
20140080136 | COMPOSITIONS, KITS, AND METHODS FOR DETECTING AND ANALYZING VESICLES - Provided are compositions, kits, and methods for detecting a vesicle comprising a membrane permeable marker that is converted into a detectable marker inside the vesicle. | 03-20-2014 |
20140093880 | COMPOSITIONS, KITS, AND METHODS FOR ISOLATING VESICLES - A composition, a kit, and a method of isolating a vesicle from a sample using a compound comprising zwitterion moieties, which may be used to analyze vesicles, and proteins, glycoprotein, lipids, or nucleic acids thereof. | 04-03-2014 |
20140099652 | COMPOSITION FOR MONITORING VESICLE, KIT AND METHOD OF MONITORING VESICLE USING THE SAME - Provided is a method of monitoring a vesicle in a sample, including contacting a vesicle in a sample with a membrane permeable marker that is converted into a detectable marker in the vesicle, measuring a signal of the detectable marker, and monitoring the vesicle based on the measured signal. | 04-10-2014 |
20140141418 | POLYNUCLEOTIDE AND USE THEREOF - Provided is a dual-hybridization polynucleotide including a first complementary region that is complementary to the 3′-terminus of a target nucleic and a second complementary region that is complementary to the 5′-terminus of the target nucleic acid, a composition and kit including the polynucleotide, and a method of producing a nucleotide sequence complementary to the target nucleic acid. The first complementary region to be bound at the 3′-terminus of the target nucleic acid can be shortened and the target nucleic acid may be amplified with excellent specificity and/or sensitivity. | 05-22-2014 |
20140178885 | COMPOSITION AND KIT FOR DIAGNOSING BREAST CANCER INCLUDING POLYNUCLEOTIDE WITHIN VESICLE, AND METHOD OF DIAGNOSING BREAST CANCER USING THE SAME - A breast cancer diagnostic composition and kit, and methods of diagnosing breast cancer or acquiring information for breast cancer diagnosis by using the composition or kit are provided. The composition or kit includes a polynucleotide that is the same as or complementary to at least one microRNA (miRNA) selected from the group consisting of hsa-miR-126, hsa-miR-23a, hsa-miR-24, hsa-miR-19b, hsa-miR-103, hsa-miR-142-3p, hsa-miR-144, hsa-miR-15a, hsa-miR-185, hsa-miR-93, and hsa-miR-30c in a vesicle, or a fragment of the microRNA. | 06-26-2014 |