Patent application number | Description | Published |
20080199968 | Method of Forming Signal Probe-Polymer - To solve a problem occurring in the PALSAR method that a polymer would be formed in the state of unbound to a captured test gene and thus affect the quantitative characteristics as a nonspecific signal, it is intended to develop a technique whereby the polymer formation is controlled in the step of forming an assembly (polymer) of probes so that the polymer is formed exclusively on a test gene to thereby improve the sensitivity and quantitative characteristics. It is found that the polymer can be quantitatively formed and a nonspecific reaction can be inhibited by, in the step of forming a polymer by reacting plural kinds of probes having abilities to complementarily bind to each other, not adding or reacting these probes at once but starting with the reaction of a first probe in one group, and then reacting the second probe in the other group followed by the reactions of probes one by one (i.e., the first probe, the second probe, and so on). | 08-21-2008 |
20080248963 | Hybridization Method - A hybridization method is provided in which an efficient hybridization reaction can be carried out. Further, there are provided, using this hybridization method, a method for detecting a target gene with high sensitivity and a signal amplifying method for markedly improving the detection sensitivity of the target gene. There is provided a hybridization method comprising the use of oligonucleotides in a reaction solution, the method comprising forming partially a reaction temperature region in the reaction solution and performing a hybridization reaction in the reaction temperature region. | 10-09-2008 |
20080318226 | Signal Amplification Method - Provided are a signal amplification method of improving signal sensitivity, qualifying properties and handling property in detection of a target gene by using a PALSAR method, a method of detecting a target gene by using the method, and an oligonucleotide probe to be used in the method. A signal amplification method in detection of a target gene using a polymer formed by the use of a plurality of kinds of oligonucleotide probes having complementary base sequence regions capable of hybridizing with each other, including labeling at least one of the plurality of kinds of oligonucleotide probes with acridinium ester for detection. | 12-25-2008 |
20090054809 | Sampling Device for Viscous Sample, Homogenization Method for Sputum and Method of Detecting Microbe - It is intended to provide: a sampling device for quantitatively sampling a liquid sample having a high viscosity such as sputum; an auxiliary jig therefor; an agent for homogenizing sputum by which sputum can be quickly treated under mild conditions and a microbe contained in the sputum can be detected at an improved stability; a homogenization device; a homogenization method; a method of detecting a microbe by using the above method; a separation device for conveniently, quickly and efficiently collecting a microbe contained in sputum; a method of collecting a microbe; and a method of detecting a microbe by using the above method. A sampling device for a viscous sample includes: a tubular body; a gasket; a long rod-like plunger; a plate-shaped nozzle that has substantially the same diameter as that of a straight section of the tubular body and is adapted to close a distal end of the tubular body; and a slit formed in the plate-like nozzle, the slit having a predetermined shape adapted to serve as an aspiration and discharge port for aspirating and discharging the viscous sample. | 02-26-2009 |
20090117560 | Method of Forming Self Assembly Substance on Microsphere and Method of Detecting Target Analyte - It is intended to provide a method, in which the sensitivity in detecting a target analyte on a microsphere can be elevated and plural items can be detected at the same time. The presence of at least one target analyte in a sample is detected by bonding a microsphere having a fluorescent substance on the surface and the target analyte to a self assembly substance formed by using two oligonucleotide probes having complementary base sequence regions which are hybridizable with each other via the target analyte, thus forming a self assembly substance-bonded particle, and then analyzing the self assembly substance-bonded particle. | 05-07-2009 |
20100151592 | METHOD OF DETECTING TARGET SUBSTANCE - Provided are a method of detecting a target substance, by which the detection sensitivity in the PALSAR method can be improved and multiple genes can be simultaneously detected, and a kit for detection. The method is a method of detecting a target substance by forming a signal probe polymer with the use of multiple dimer probes or dimer-forming probes and an assist probe, in which each dimer probe is made up of two kinds of dimer-forming probes including a 5′-side region, a central region and a 3′-side region (the central regions of the two kinds of dimer-forming probes are complementary to each other and the 3′-side regions and the 5′-side regions thereof are not complementary to each other), the multiple dimer probes are constituted such that a dimer probe polymer is formed via a self-assembly reaction, and the assist probe is designed so as to have a structure having a region complementary to the 5′-side region of one dimer-forming probe in one dimer probe, a region complementary to the 3′-side region of the other dimer-forming probe, and a target region capable of binding to the target substance. | 06-17-2010 |
20100160179 | Method of detecting target substance - Provided are a method of detecting a target substance whereby the detection sensitivity in the PALSAR method can be improved and multiple genes can be simultaneously detected, and a kit for detection. The method of detecting a target substance is a method of detecting a target substance by forming a signal probe polymer using one or more sets of paired dimer-forming probes for forming dimer probes or dimer probes, one or more kinds of crosslinking probes, and one or more kinds of assist probes, in which a dimer-forming probe includes a 5′-side region, a central region, and a 3′-side region, a crosslinking probe includes two regions, i.e., a 5′-side region and a 3′-side region, the 5′-side region of the dimer-forming probe is complementary to either 5′-side region of a crosslinking probe, the 3′-side region of the dimer-forming probe is complementary to either 3′-region of a crosslinking probe, and an assist probe includes a region complementary to the 5′-side region in one of paired dimer-forming probes, a region complementary to the 3′-side region in the other, and a target region capable of binding to the target substance. | 06-24-2010 |
20100248222 | METHOD OF FORMING SIGNAL PROBE-POLYMER - Provided are a method of forming a signal probe-polymer which makes it possible to form a polymer efficiently and quantitatively, a signal probe-polymer formed by the method, oligonucleotide probes for use in the method, and a method of detecting target analyte having high sensitivity and excellent quantitative capability. The method of forming a signal probe-polymer comprises reacting a plurality of pairs of oligonucleotide probes with each other to form a polymer, a first probe of the pair of oligonucleotide probes comprising three nucleic acid regions of X, Y, and Z regions, located in the stated order from the 5′-terminal and a second probe comprising three nucleic acid regions of X′, Y′, and Z′ regions, located in the stated order from the 5′-terminal, wherein each region of the oligonucleotide probes has a length of from 13 to 15 bases. | 09-30-2010 |
20110171639 | POLYMER FOR DETECTION OF TARGET SUBSTANCE, AND METHOD FOR DETECTION OF TARGET SUBSTANCE - Provided is a method of detecting a target substance by which the target substance can be detected with efficiency and a high sensitivity, and in a simple manner, a target substance detection polymer used in the method, and a method of forming the polymer. The method of detecting a target substance includes the steps of: (A) forming a target substance detection polymer by causing multiple kinds of nucleic acid probes for forming a polymer to react with a binding probe having a region capable of binding to the target substance and a region capable of binding to at least one of the nucleic acid probes in a solution; (B) binding the target substance detection polymer and the target substance; and (C) detecting the target substance detection polymer to which the target substance is bound. | 07-14-2011 |