Patent application number | Description | Published |
20100284016 | OPTICAL CYTOMETRY - The present invention provides optical systems and methods for determining a characteristic of a cell, such as cell type, cellular response to a biochemical event, biological state and the like. The methods typically involve using interferometry to observe membrane properties in a cell and then use this information to determine one or more characteristics of a cell. The methods of the invention are useful for applications such as drug screening as well as diagnostic techniques. | 11-11-2010 |
20110117648 | SINGLE CELL SURGERY TOOL AND A CELL TRANSFECTION DEVICE UTILIZING THE PHOTOTHERMAL PROPERTIES OF THIN FILMS AND/OR METAL NANOPARTICLES - This invention provides novel tools for surgery on single cells. In certain embodiments the tools comprise a microcapillary having at and/or near the tip a metal coating or a plurality of nanoparticles that can be heated by application of electromagnetic energy. In certain embodiments substrates are provided that facilitate the introduction of agents into cells. The substrates typically comprise a surface bearing a film or particles or nanoparticles that can be heated by application of electromagnetic energy. | 05-19-2011 |
20120236299 | HIGH-SPEED ON DEMAND DROPLET GENERATION AND SINGLE CELL ENCAPSULATION DRIVEN BY INDUCED CAVITATION - Methods and devices for the formation of droplets of a first fluid in a second fluid and the encapsulation of particles or cells within such droplets are disclosed. Impetus for droplet formation is provided by the creation of a transient bubble, which may be induced using a pulsed laser. Droplet volume and the frequency at which droplets are formed can be controlled by modulation of the pulsed laser. The disclosed methods and devices are particularly suitable for use in microfluidic devices. | 09-20-2012 |
20120283317 | Methods and Compositions for Regulating RNA Import into Mitochondria - Disclosed herein recombinant and/or isolated nucleic acid molecules which comprise, consist essentially of, or consist of a first nucleic acid sequence, which is a wild-type sequence or an altered sequence, directly or indirectly linked to a second nucleic acid sequence selected from the group consisting of: (1) a mitochondria localization sequence, (2) an RNA import sequence, or (3) a combination thereof, and methods of using thereof. | 11-08-2012 |
20140080171 | OPTICAL CYTOMETRY - The present invention provides optical systems and methods for determining a characteristic of a cell, such as cell type, cellular response to a biochemical event, biological state and the like. The methods typically involve using interferometry to observe membrane properties in a cell and then use this information to determine one or more characteristics of a cell. The methods of the invention are useful for applications such as drug screening as well as diagnostic techniques. | 03-20-2014 |
20140178865 | RAPID, MASSIVELY PARALLEL SINGLE-CELL DRUG RESPONSE MEASUREMENTS VIA LIVE CELL INTERFEROMETRY - A central question in cancer therapy is how individual cells within a population of tumor cells respond to drugs designed to arrest their growth. However, the absolute growth of cells, their change in physical mass, whether cancerous or physiologic, is difficult to measure directly with traditional techniques. Embodiments of the invention provide live cell interferometry (LCI) for rapid, realtime quantification of cell mass in cells exposed to a changing environment. Overall, LCI provides a conceptual advance for assessing cell populations to identify, monitor, and measure single cell responses, such as to therapeutic drugs. | 06-26-2014 |
20150044751 | PHOTOTHERMAL SUBSTRATES FOR SELECTIVE TRANSFECTION OF CELLS - This invention provides novel tools for surgery on single cells and substrates/devices for delivery of reagents to selected cells. In certain embodiments the substrates comprise a surface comprising one or more orifices, where nanoparticles and/or a thin film is deposited on a surface of said orifice or near said orifice, where the nanoparticles and/or a thin film are formed of materials that heat up when contacted with electromagnetic radiation. In certain embodiments the pores are in fluid communication with microchannels containing one or more reagents to be delivered into the cells. | 02-12-2015 |
20150197720 | PHOTOTHERMAL SUBSTRATES FOR SELECTIVE TRANSFECTION OF CELLS - This invention provides novel tools for surgery on single cells and substrates/devices for delivery of reagents to selected cells. In certain embodiments the substrates comprise a surface comprising one or more orifices, where nanoparticles and/or a thin film is deposited on a surface of said orifice or near said orifice, where the nanoparticles and/or a thin film are formed of materials that heat up when contacted with electromagnetic radiation. In certain embodiments the pores are in fluid communication with microchannels containing one or more reagents to be delivered into the cells. | 07-16-2015 |