Patent application number | Description | Published |
20080238627 | Sample carrier device incorporating radio frequency identification, and method - An apparatus for associating information with a carrier and/or at least one biological reagent is provided. The apparatus can include a carrier adapted to retain at least one biological reagent and at least one non-silicon Radio Frequency Identification Tag (RFID). The RFID tag can comprise information in the form of an information pointer that can be resolved by an information pointer resolution device. The information pointer and/or resolution device can be used to access a remote system. The carrier can be configured, for example, as a microfluidic processing device or a multi-well plate. Methods and systems using the apparatus are also provided. | 10-02-2008 |
20080240529 | Methods for Improving the Perfomance of a Detector - A system and method for allowing adjustments of various parameters associated with the operation of charge-coupled devices adapted for use in biological analysis devices. Gate voltage signal applied to the CCDs can be adjusted to advantageously affect the manner in which charge is transferred from pixels. The manner in which transferred charges from the pixels are processed in a readout process can also be adjusted to advantageously change the speed of the CCD based detector. Various methods of performing such adjustments are disclosed. | 10-02-2008 |
20080265139 | COMBINATION READER - An imaging system for collecting images of signals associated with a sample tile comprising a stage supporting the sample tile, a ring illuminator system emitting a uniform excitation energy upon an entirety of the sample tile causing at least a first signal to be generated from the sample tile, and an image collecting device collecting a first image of the first signal. The image collecting device further collecting a second image of a second signal emitted from the sample tile, wherein the second signal being different than the first signal. | 10-30-2008 |
20080288179 | NORMALIZATION OF DATA - Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers. | 11-20-2008 |
20080290283 | Signal noise reduction for imaging in biological analysis - A system and method for characterizing contributions to signal noise associated with charge-coupled devices adapted for use in biological analysis. Dark current contribution, readout offset contribution, photo response non-uniformity, and spurious charge contribution can be determined by the methods of the present teachings and used for signal correction by systems of the present teachings. | 11-27-2008 |
20080308160 | Device including inductively heatable fluid retainment region, and method - A device is provided that comprises one or more fluid retainment regions each having at least one wall, and one or more loops in heat-transfer communication with the at least one wall. Each of the loops can comprise an electrical conductor that surrounds the same or a different fluid retainment region. A device is provided that comprises one or more fluid retainment regions each having particulates disposed therein. A system is provided that includes a platen adapted to hold a device including fluid retainment regions and one or more electrical conductors in heat-transfer communication with the fluid retainment regions. Methods of heating a sample are also provided. | 12-18-2008 |
20080316482 | Optical scanning configurations, systems, and methods - Methods and optical systems for scanning of a target sample, including methods and systems using a low mass scan head. The present invention also relates to methods and systems for performing sample assays, and for producing and measuring optical responses and signatures. | 12-25-2008 |
20090000690 | Diffusion-aided loading system for microfluidic devices - Microfluidic devices having a diffusion-aided system for loading samples into the microfluidic device are provided. Methods of gas-venting a microfluidic device through a non-porous, gas permeable material sealing cover layer, for example, during liquid sample loading, are also provided. The non-porous, gas-permeable material can be, for example, a polysiloxane, for example, polydimethylsiloxane. | 01-01-2009 |
20090009767 | Optical instrument including excitation source - An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided. | 01-08-2009 |
20090021735 | Time-delay integration in detection of labeled beads - An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector. | 01-22-2009 |
20090130666 | Cytometric system including nucleic acid sequence amplification, and method - The invention relates to a cytometric system that can include a first station capable of selectively sorting a nucleic acid sequence-containing biological material from a biological sample, and a second station where nucleic acid of the sorted nucleic acid sequence-containing biological material, can undergo a nucleic acid amplification reaction. The system can include a conduit system adapted to facilitate transfer of a sorted biological material or processed biological material from one region or station, to another. All of the stations can be controlled by a central control system. Methods of sorting a biological material from a biological sample, and methods of amplifying nucleic acid of a sorted nucleic acid sequence-containing material, are also provided. | 05-21-2009 |
20090135421 | System for detecting markers - An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector. | 05-28-2009 |
20090140128 | Methods, systems and apparatus for light concentrating mechanisms - An embodiment relates generally to resonant structure. The resonant structure includes a substrate and a nano-bowtie antenna deposited over the substrate. The resonant structure also includes an enclosure deposited over the substrate and surrounding the nano-bowtie antenna, where the enclosure is configured to raise an enhancement level in the nano-bowtie antenna. | 06-04-2009 |
20090141272 | Optical instrument comprising multi-notch beam splitter - An instrument is provided that can monitor nucleic acid sequence amplification reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path. | 06-04-2009 |
20090250615 | Scanning system and method for imaging and sequencing - A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided. | 10-08-2009 |
20090305287 | Method and System for Multiplex Genetic Analysis - A method for identifying nucleotides in a nucleic acid sequence is disclosed. A plurality of polymerization complexes are provided within a plurality of confined reaction environments. Each complex comprises a polymerase enzyme and a template nucleic acid. The plurality of complexes are contacted with a plurality of types of nucleotide analogs labeled with distinguishable fluorescent labels under conditions suitable for polymerization. Fluorescent signals associated with incorporation of a nucleotide analog are transmitted to a detector, wherein the location of the fluorescent signal on the detector is indicative of the individual confined reaction environment and the type of nucleotide incorporated. The nucleotide in a nucleic acid sequence is identified based upon the type of nucleotide incorporated and the confined reaction environment. | 12-10-2009 |
20090305908 | Method and System for Multiplex Genetic Analysis - A method for preferentially localizing desired molecules within an optical confinement disposed upon a substrate is disclosed. The desired molecules are deposited over the surface of the substrate. The desired molecules that are not within the optical confinement are selectively removed from the surface of the substrate. | 12-10-2009 |
20090305909 | Method and System for Multiplex Genetic Analysis - A method for selectively immobilizing a molecule of interest on a substrate is disclosed. A substrate having a first surface component and a second surface component is provided. The first and second surface components have different surface characteristics. The molecule of interest is selectively coupled to the first surface component based upon a difference between the surface characteristics of the first surface component and the surface characteristics of the second surface component. | 12-10-2009 |
20100036616 | Normalization of Gene Expression Data - A method for determining bias across two domains comprising gene expression data. The method can comprise (a) providing a first domain and a second domain; (b) obtaining information indicative of a bias within the first domain; (c) obtaining information indicative of a bias within the second domain; and (d) using the information indicative of the bias within the first domain and the information indicative of the bias within the second domain to produce an indication of bias across the two domains. | 02-11-2010 |
20100103416 | DNA Sequencing System - An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector. | 04-29-2010 |
20100113285 | Normalization of Data Using Controls - A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample. | 05-06-2010 |
20100163710 | COMBINATION READER - An imaging system for collecting images of signals associated with a sample tile comprising a stage supporting the sample tile, a ring illuminator system emitting a uniform excitation energy upon an entirety of the sample tile causing at least a first signal to be generated from the sample tile, and an image collecting device collecting a first image of the first signal. The image collecting device further collecting a second image of a second signal emitted from the sample tile, wherein the second signal being different than the first signal. | 07-01-2010 |
20100178691 | Systems, Methods, and Apparatus for Single Molecule Sequencing - An embodiment generally relates to a system for analysis of an analyte. The system can include a transparent substrate. The system also includes an excitation light source configured to induce an evanescent wave excitation of a fluorescently labeled molecule near the access to the transparent substrate and a detector for detecting the fluorescently labeled molecule. | 07-15-2010 |
20100179075 | PARTICLES FOR USE IN SUPPORTED NUCLEIC ACID LIGATION AND DETECTION SEQUENCING - Compositions and methods to modify the surface of particles to which biomolecules are attached are disclosed. The particles can include beads and nanoparticles which are composed of metallics, metal alloys, glass, polymers and derivatives and composites thereof. The surface of the particles are modified to be hydrophilic for ease in the attachment of biomolecules to the particle surface and immobilization of the particles to a substrate to facilitate process such as nucleic acid sequencing, PCR and sequencing by ligation. | 07-15-2010 |
20100193672 | Optical Camera Alignment - A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens. | 08-05-2010 |
20100216143 | Apparatus and Method for Differentiating Multiple Fluorescence Signals by Excitation Wavelength - An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers. | 08-26-2010 |
20100220321 | Normalization of Data - Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers. | 09-02-2010 |
20100261158 | Method and System for Multiplex Genetic Analysis - The present disclosure provides apparatus, systems and method for detecting separately and substantially simultaneously light emissions from a plurality of localized light-emitting analytes. A system according to exemplary embodiments of the present disclosure comprises a sample holder having structures formed thereon for spatially separating and constraining a plurality of light-emitting analytes each having a single nucleic acid molecule or a single nucleic acid polymerizing enzyme, a light source configured to illuminate the sample holder, an optical assembly configured to collect and detect separately and substantially simultaneously light emissions associated with the plurality of light emitting analytes. The system may further include a computer system configured to analyze the light emissions to determine the structures or properties of a target nucleic acid molecule associated with each analyte. | 10-14-2010 |
20110001609 | SAMPLE CARRIER DEVICE INCORPORATING RADIO FREQUENCY IDENTIFICATION, AND METHOD - An apparatus for associating information with a carrier and/or at least one biological reagent is provided. The apparatus can include a carrier adapted to retain at least one biological reagent and at least one non-silicon Radio Frequency Identification Tag (RFID). The RFID tag can comprise information in the form of an information pointer that can be resolved by an information pointer resolution device. The information pointer and/or resolution device can be used to access a remote system. The carrier can be configured, for example, as a microfluidic processing device or a multi-well plate. Methods and systems using the apparatus are also provided. | 01-06-2011 |