Patent application number | Description | Published |
20080268497 | TREATMENT SOLUTION, METHOD FOR PRETREATMENT, METHOD FOR ACQUIRING INFORMATION AND METHOD FOR DETECTION - The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens μm and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis. | 10-30-2008 |
20080277576 | TIME-OF-FLIGHT SECONDARY ION MASS SPECTROMETER - A time-of-flight secondary ion mass spectrometer comprises an ion source which generates cluster ions each comprised of two or more atoms, a pulsing mechanism which pulses the cluster ions, a selecting mechanism which selects ions having a specific mass number from the pulsed cluster ions and passes the selected ions in an ON state of the selecting mechanism, and, passes the pulsed cluster ions without the selecting in an OFF state of the selecting mechanism, and a time-of-flight mass spectrometric unit which measures a mass spectrum of secondary ions generated from a sample using a difference in time of flight when the sample is irradiated with the ions passed through the selecting mechanism. | 11-13-2008 |
20080278706 | METHOD FOR PRE-TREATING SPECIMEN AND METHOD FOR ANALYZING SPECIMEN - A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method. | 11-13-2008 |
20080290268 | INFORMATION OBTAINING METHOD - An information obtaining method for obtaining information about a mass of a component of an analyte using a time of flight mass spectrometer and obtaining information about a distribution state of the component based on the obtained information about the mass includes the step of: (1) adding a self-reactive substance to the analyte on a base to facilitate ionization of the component; (2) irradiating the analyte with a primary beam in the presence of the self-reactive substance, thereby ionizing the components and allowing resulting ions to fly; (3) obtaining information about mass of the flying ions using the time of flight mass spectrometer; and (4) obtaining information about the distribution state of the component on the base based on the information about the mass. | 11-27-2008 |
20090026364 | METHOD OF MEASURING TARGET OBJECT IN A SAMPLE USING MASS SPECTROMETRY - In-plane distribution of a target object contained in a sample is measured. The sample dispersedly placed on a substrate is treated to promote ionization of the target object. Then, the mass and flying amount of an ion containing the target object or a component thereof is determined by irradiating an ion beam to the sample and performing time-of-flight secondary ion mass spectrometry of the ion that flies from a portion in the sample where the ion beam is irradiated, and the in-plane distribution of the target object is determined from the mass and flying amount data obtained at plural portions by scanning the beam on the sample plane. The step of treating the sample to promote ionization of the target object includes contacting an aqueous solution of an acid that does not crystallize at ordinary temperature with the sample. A high spatial resolution two-dimensional image can be obtained. | 01-29-2009 |
20090266982 | MASS SPECTROMETRY SUBSTRATE AND MASS SPECTROMETRY METHOD - A mass spectrometry method that makes it possible to perform high-sensitivity detection of a desorbed/ionized object substance to be measured in mass spectrometry in which the object substance to be measured is desorbed and ionized. The method includes placing at least an ionizing agent having two or more functional groups represented by Formula (1) below in a molecule: | 10-29-2009 |
20100227308 | INFORMATION ACQUISITION METHOD, INFORMATION ACQUISITION APPARATUS AND DISEASE DIAGNOSIS METHOD - An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry. | 09-09-2010 |
20110248156 | INFORMATION ACQUIRING APPARATUS AND INFORMATION ACQUIRING METHOD FOR ACQUIRING MASS-RELATED INFORMATION - Target molecules in a sample can be detected at an improved sensitivity by means of a mass spectrometer. A sample with or without a matrix is placed on a substrate and irradiated with a converged and pulsed primary beam selected from an ion beam, a neutral particle beam or a laser beam. Secondary ions and neutral molecules are emitted along with protons from the irradiated point of the sample as an electric field is applied between the substrate and an extraction electrode disposed above the substrate. A proton-control electrode is arranged in axial symmetry with the trajectory of the primary beam. A voltage is applied thereto so that the generated electric field decelerates the flying protons to raise their adhering efficiency to the flying neutral molecules. | 10-13-2011 |
20110315871 | METHOD OF FORMING MASS IMAGE - An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level. | 12-29-2011 |
20120298859 | METHOD AND APPARATUS FOR REDUCING NOISE IN MASS SIGNAL - A more effective noise reduction method is provided. In the method, when mass spectrum information having a spatial distribution is processed, the whole data is taken as three-dimensional data (positional information is stored in an xy plane, and spectral information is stored along a z-axis direction), and three-dimensional wavelet noise reduction is performed by applying preferable basis functions to a spectral direction and a peak distribution direction (in-plane direction). | 11-29-2012 |
20120301968 | MASS SPECTROMETRY METHOD - The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry. | 11-29-2012 |
20130158882 | METHOD OF FORMING MASS IMAGE - An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level. | 06-20-2013 |
20130203105 | METHOD FOR PRE-TREATING SPECIMEN AND METHOD FOR ANALYZING SPECIMEN - A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method. | 08-08-2013 |
20130320204 | SAMPLE ANALYSIS METHOD AND ANALYZER - Provided is a sample analysis method of irradiating a sample with a primary ion beam to analyze a secondary ion emitted from the sample by mass spectrometry, the sample analysis method including the steps of cooling a sample placed in a chamber; forming an ice layer on a surface of the cooled sample by discharging one of water and an aqueous solution to the chamber; and irradiating the surface of the sample with the primary ion beam with the ice layer being formed thereon, wherein an amount of the water forming the ice | 12-05-2013 |
20140087408 | INFORMATION ACQUISITION METHOD - The present invention relates to an information acquisition method of acquiring information of distribution of a protein or peptide in a sample based on mass information obtained by mass spectrometry of the protein or peptide. The method includes mass spectrometry of a definite region of the sample after limited proteolysis of the protein or peptide and acquisition of information relating to distribution using an ion peak that has a two-dimensional intensity distribution having a Pearson product-moment correlation coefficient of 0.5 to more and 1.0 or less in the definite region against the two-dimensional intensity distribution of the parent ion of the protein or the peptide subjected to the limited proteolysis and has a peak intensity ratio of larger than 1.0 against the peak intensity of the integrated spectrum of the parent ion in the definite region, wherein the m/z of the ion peak is greater than 500. | 03-27-2014 |
Patent application number | Description | Published |
20090251691 | Liquid Immersion Microscope - To provide a liquid immersion microscope device enabling nondestructive liquid immersion observation of a substrate without deteriorating quality of the substrate. To attain this, a liquid immersion microscope device of the present invention includes a supporting unit supporting a substrate as an observation target, an objective lens of a liquid immersion type, a first supplying unit supplying ultrapure water as a liquid for observation to a gap between a tip of the objective lens and the substrate, a first draining unit draining the liquid for observation after observation of the substrate, a second supplying unit supplying a liquid for cleaning, which is different from the liquid for observation drained by the first draining unit, to an area, of the substrate, that has been in contact with the liquid for observation, and a second draining unit draining the liquid for cleaning after the substrate is cleaned. | 10-08-2009 |
20120105867 | PROFILE MEASURING APPARATUS, METHOD FOR MANUFACTURING STRUCTURE, AND STRUCTURE MANUFACTURING SYSTEM - There is provided a profile measuring apparatus which measures a profile of an object including: a projection unit which projects a pattern on the object from a projection direction; a measurement unit, which is displaced at a difference position for the projection unit and takes an image of the pattern from a direction different from the projection direction to measure a position on a surface of the object based on an image data obtained with the taken image; an object-rotation unit which rotates the object in two directions; and a pattern-rotation unit which is connected to the projection unit so as to be able to rotate the pattern relative to the object-rotation unit. | 05-03-2012 |