Lyznik
Anna Lyznik, Johnston, IA US
Patent application number | Description | Published |
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20090307800 | Compositions and Methods of Use of Mitogen-Activated Protein Kinase Kinase Kinase - Methods and compositions for modulating plant response, development and yield under varying environmental conditions are provided. Methods employing MAPKKK are provided. The MAPKKK sequences are used in a variety of methods including modulating root development, modulating leaf and/or shoot development, modulating tolerance under abiotic stress and modulating yield. Transformed plants, plant cell, tissues, seed and expression vectors are also provided. | 12-10-2009 |
20130133111 | COMPOSITIONS AND METHODS OF USE OF MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE - Methods and compositions for modulating plant response, development and yield under varying environmental conditions are provided. Methods employing MAPKKK are provided. The MAPKKK sequences are used in a variety of methods including modulating root development, modulating leaf and/or shoot development, modulating tolerance under abiotic stress and modulating yield. Transformed plants, plant cell, tissues, seed and expression vectors are also provided. | 05-23-2013 |
L. Aleksander Lyznik, Johnston, IA US
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20090133152 | METHODS FOR ALTERING THE GENOME OF A MONOCOT PLANT CELL - Methods and compositions for altering the genome of a monocot plant cell, and a monocot plant are disclosed. The methods and compositions use a double-strand break inducing agent to alter a monocot plant or plant cell genomic sequence comprising a recognition sequence for the double-strand break inducing agent. | 05-21-2009 |
20150074843 | Methods For Altering The Genome Of A Monocot Plant Cell - Methods and compositions for altering the genome of a monocot plant cell, and a monocot plant are disclosed. The methods and compositions use a double-strand break inducing agent to alter a monocot plant or plant cell genomic sequence comprising a recognition sequence for the double-strand break inducing agent. | 03-12-2015 |
20150082478 | PLANT GENOME MODIFICATION USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS AND METHODS OF USE - Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. | 03-19-2015 |
L. Alexander Lyznik, Johnston, IA US
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20080216200 | Alternative splicing factors polynucleotides, polypeptides and uses thereof - The invention provides isolated polynucleotides and their encoded proteins that are involved in splicing or modulating splicing activity. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering splicing protein content and/or composition of plants. | 09-04-2008 |
Lesek Alexander Lyznik, Johnston, IA US
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20080282426 | MOBILIZATION OF VIRAL GENOMES FROM T-DNA USING SITE-SPECIFIC RECOMBINATION SYSTEMS - The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise | 11-13-2008 |
Leszek A. Lyznik, Johnston, IA US
Patent application number | Description | Published |
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20100173801 | Modified FRT Recombination Site Libraries and Methods of Use - Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest. | 07-08-2010 |
20100192263 | Novel FRT Recombination Sites and Methods of Use - Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided. | 07-29-2010 |
20110047655 | NOVEL FRT RECOMBINATION SITES AND METHODS OF USE - Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided. | 02-24-2011 |
20130052739 | NOVEL FRT RECOMBINATION SITES AND METHODS OF USE - Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided. | 02-28-2013 |
20140338070 | METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT - Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. | 11-13-2014 |
20150044771 | Novel FRT Recombination Sites and Methods of Use - Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided. | 02-12-2015 |
Leszek Aleksander Lyznik, Johnston, IA US
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20140020131 | METHODS AND COMPOSITIONS FOR PRODUCING MALE STERILE PLANTS - Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed. | 01-16-2014 |