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| 20080229438 | Process of making transgenic mammals that produce exogenous proteins in milk and transgenic mammals produced thereby - The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product. | 09-18-2008 |
| 20110072526 | PROCESS FOR PRODUCING EXOGENOUS PROTEIN IN THE MILK OF TRANSGENIC MAMMALS AND A PROCESS FOR PURIFYING PROTEINS THEREFROM - The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct comprising the hGH gene and beta casein promoter sequences conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product. | 03-24-2011 |
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| 20100193346 | PROCESS FOR THE PURIFICATION OF AN AQUEOUS STREAM COMING FROM THE FISCHER-TROPSCH REACTION - The present invention relates to a process for the purification of an aqueous stream coming from the Fischer-Tropsch reaction which comprises:—feeding of the aqueous stream containing the organic by-products of the reaction to a system consisting of a distillation column equipped with a partial condenser and a total condenser;—partial condensation of the vaporized stream leaving the head of the column and collection of a first distillate enriched in the heavier by-products;—total condensation of the vaporized stream leaving the partial condenser and collection of a liquid stream which is partly sent back to the distillation column as reflux whereas the remaining part is collected as distillate;—extraction of the purified aqueous stream from the bottom of the distillation column. | 08-05-2010 |
| 20110100819 | PROCESS FOR THE TREATMENT OF THE AQUEOUS STREAM COMING FROM THE FISCHER-TROPSCH REACTION - The present invention relates to a process for the treatment of the aqueous stream coming from the Fischer-Tropsch reaction which comprises: —feeding of the aqueous stream containing organic by-products of the reaction to a distillation or stripping column; —collection from the column of a distillate enriched in alcohols having from 1 to 8 carbon atoms and other possible volatile compounds; feeding of the aqueous stream containing the acids leaving the bottom of the distillation column to an electrodialysis cell and the production of two outgoing streams: —an aqueous stream (i) enriched in organic acids having from 1 to 8 carbon atoms; —a purified aqueous stream (ii) with a low acid content. | 05-05-2011 |
| 20110127155 | PROCESS FOR THE TREATMENT OF THE AQUEOUS STREAM COMING FROM THE FISCHER-TROPSCH REACTION - The present invention relates to a process for the treatment of the aqueous stream coming from the Fischer-Tropsch reaction, comprising: —feeding the aqueous stream ( | 06-02-2011 |
| 20120055868 | PROCESS FOR THE PURIFICATION OF AN AQUEOUS STREAM COMING FROM THE FISCHER-TROPSCH REACTION - Process for the purification of an aqueous stream coming from the Fischer-Tropsch reaction which comprises: —feeding said aqueous stream containing the organic by-products of the reaction to a distillation column, obtaining two output streams: —an aqueous stream (i) exiting from the column head, enriched with alcohols having from 1 to 20 carbon atoms, preferably from 1 to 9 carbon atoms, and other optional volatile compounds; an aqueous stream (ii) exiting from the column bottom, enriched with organic acids having from 1 to 10 carbon atoms, preferably from 2 to 6 carbon atoms; —subjecting said aqueous stream (ii) to an anaerobic biological treatment obtaining a purified aqueous stream (iii); wherein said anaerobic biological treatment is carried out with a hydraulic retention time lower than 15 hours, preferably ranging from 4 hours to 10 hours, more preferably ranging from 5 hours to 8 hours. | 03-08-2012 |
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| 20090263359 | Particulate Delivery Vehicles for Embryoid Bodies - The present invention provides a vehicle for delivering various chemicals, compositions and proteins to stem cells and embryoid bodies. The vehicle may be biocompatible and biodegradable polymer microparticles. Typically the particles will contain at least a growth factor for delivery to the embryoid bodies, and generally the growth factor induces differentiation of the cells in the embryoid body along a specific lineage. The present invention also provides methods for directing differentiation of the cells in the embryoid body. | 10-22-2009 |
| 20100215708 | COATING OF DEVICES WITH EFFECTOR COMPOUNDS - This invention is directed to substrates, materials and devices coated with a gel, foam, film, particle or composition comprising a polymei solvent and effector compounds attached thereto, processes of producing the same, and methods of use thereof, of in, inter-alia, biological applications, including preventing infection and the treatment of various diseases. | 08-26-2010 |
