Patent application number | Description | Published |
20080280340 | Method and Apparatus for Bacterial Transformation by Electroporation with Waveforms Incorporating Pulsed Rf Between 3 and 125 Mhz - A method and apparatus for electroporation includes placing a mixture of bacterial suspension and transforming DNA into an electroporation cuvette. The resulting sample is subjected through a current-limiting device to a complex 5 waveform including a burst of high-voltage radio-frequency current, which in some embodiments is superimposed on a biphasic high-voltage DC pulse, and in other embodiments on a high-voltage lower-frequency AC burst. The total waveform has at least an initial portion greater than eleven thousand volts per centimeter of electrode spacing, and a later portion in some embodiments is reduced to less than thirty percent 10 of magnitude of the initial portion. Transformed bacteria are selected by culture in selective medium in an embodiment. The high-voltage radio-frequency current is between 3 and 125 MHz, and in an embodiment is 24 MHz. | 11-13-2008 |
20090017503 | Method and Apparatus for Saccharide Precipitation From Pretreated Lignocellulosic Materials - A method for separating saccharide components and lignin fractions from a concentrated acid treated lignocellulosic biomass is disclosed. The method involves precipitating the saccharide components by adding an organic solvent to the biomass slurry. The acid may then be recovered, for example, by filtration or by countercurrent washing and the organic solvent may be flashed and recycled. During acid recovery and organic recovery steps, two main lignocellulose components (hemicellulose and lignin) as well as minor components such as acetic acid are separated as well. The method decreases the amount of cellulase required for hydrolysis, increases hydrolysis rates, reduces formation of inhibitor molecules, increase sugar yields, produces high value by-products such as high quality lignin and hemicellulose, and decreases energy and equipment costs. | 01-15-2009 |
20090221042 | Process for the Treatment of Lignocellulosic Biomass - A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production. | 09-03-2009 |
20090221049 | Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol - Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of | 09-03-2009 |
20090239277 | Thermophillic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol - Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of | 09-24-2009 |
20100015678 | Thermophilic Organisms For Conversion of Lignocellulosic Biomass To Ethanol - Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of | 01-21-2010 |
20100075363 | Recombinant Yeast Strains Expressing Tethered Cellulase Enzymes - Recombinant yeast strains that saccharify, ferment and grow on insoluble and crystalline forms of cellulose are disclosed herein. The yeast strains express tethered cellulases including cellobiohydrolase, endoglucanase and β-glucosidase. The recombinant organisms are particularly suited for consolidated bioprocessing. | 03-25-2010 |
20110244539 | Selection Of Cellulolytic Microbes With High Growth Rates - Methods for obtaining cellulolytic microbes with high growth rates are disclosed. For example, | 10-06-2011 |
20120077239 | Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol - Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of | 03-29-2012 |
20120264183 | Cellulose and Xylan Fermentation by Novel Anaerobic Thermophilic Clostridia Isolated From Self-Heated Biocompost - A new species of an anaerobic thermophilic cellulolytic and xylano lytic bacterium is disclosed. One particular strain of this new species has been deposited with the ATCC under Deposit No. PTA-10114. It is also provided a method for isolating, culturing and utilizing this novel bacterium for the conversion of biomass to bioconversion products, such as ethanol. | 10-18-2012 |
20130302870 | Flowthrough Pretreatment Of Lignocellulosic Biomass And Selective Separation Of Components Using High-Temperature Nanoporous Membranes - A new and improved biomass conversion system is disclosed using high-temperature flow-though pretreatment and a nanoporous membrane to provide more digestible biomass for subsequent conversion to biofuels. | 11-14-2013 |