Patent application number | Description | Published |
20120276628 | AUTOMATED SYSTEMS AND METHODS FOR ISOLATING REGENERATIVE CELLS FROM ADIPOSE TISSUE - A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer; introducing the adipose tissue into the vessel; introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided. | 11-01-2012 |
20130289257 | FORMULATIONS FOR NUCLEIC ACID STABILIZATION ON SOLID SUBSTRATES - The present disclosure generally relates to dry solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described. | 10-31-2013 |
20130289265 | METHODS AND COMPOSITIONS FOR EXTRACTION AND STORAGE OF NUCLEIC ACIDS - The present disclosure generally relates to solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for extracting, collecting, and recovering nucleic acids from the solid compositions are also described. | 10-31-2013 |
20150031035 | METHOD AND DEVICE FOR COLLECTION AND AMPLIFICATION OF CIRCULATING NUCLEIC ACIDS - Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane. | 01-29-2015 |
20150031086 | LIGASE-ASSISTED NUCLEIC ACID CIRCULARIZATION AND AMPLIFICATION - Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided. | 01-29-2015 |
Patent application number | Description | Published |
20130224770 | Antibody Diluent Buffer - The compositions described include an antibody diluent buffer that minimizes the amount of antibody required for immunoassays and further improves the performance of antibody-based assays. The claimed antibody diluent buffer comprises a high-molecular weight neutral polymer and an antibody that specifically binds to an antigen of interest, wherein the antibody remains in solution in the presence of the polymer. Methods for the use of the antibody diluent buffer to detect an antigen, even trace amounts of an antigen, in an antibody-based assay are also disclosed herein. | 08-29-2013 |
20130338351 | METHODS AND COMPOSITIONS FOR EXTRACTION AND STORAGE OF NUCLEIC ACIDS - A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described. | 12-19-2013 |
20140234942 | SUBSTRATES AND METHODS FOR COLLECTION, STABILIZATION AND ELUTION OF BIOMOLECULES - A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described. | 08-21-2014 |
Patent application number | Description | Published |
20120217831 | SYSTEMS AND METHODS FOR IMPROVED DIRECT DRIVE GENERATORS - Systems and methods for improved generators are described. One embodiment includes an assembly for an axial flux generator, the assembly comprising an arc-shaped rotor section that includes a rotor support configured to rotate around an axis of a generator, and an at least one rotor element for power generation that is connected to the rotor support; an arc-shaped stator section comprising a stator support and an at least one stator element for power generation connected to the stator support; wherein the rotor section and the stator section are configured to provide an axial air gap between the at least one rotor element and the at least one stator element, and wherein the at least one rotor element and the at least one stator element are positioned to be at substantially the same radial distance from the axis of the generator. | 08-30-2012 |
20120262095 | AIR GAP CONTROL SYSTEMS AND METHODS - In one embodiment, an apparatus includes a first member that supports a magnetic flux carrying member and a second member that supports a magnetic flux generating member disposed for movement relative to the first member. An air gap control system is coupled to at least one of the first member or the second member and includes an air gap control device that is separate from a primary magnetic flux circuit formed between the first member and the second member. The air gap control device is configured to exert a force on one of the first and second members in response to movement of the other of the first and second members in a direction that reduces a distance between the first and second members to maintain a minimum distance between the first and second members and/or substantially center the one of the first and second members within the other. | 10-18-2012 |
20130119802 | AIR GAP CONTROL SYSTEMS AND METHODS - In one embodiment, an apparatus includes a first member that supports a magnetic flux carrying member and a second member that supports a magnetic flux generating member disposed for movement relative to the first member. An air gap control system is coupled to at least one of the first member or the second member and includes an air gap control device that is separate from a primary magnetic flux circuit formed between the first member and the second member. The air gap control device is configured to exert a force on one of the first and second members in response to movement of the other of the first and second members in a direction that reduces a distance between the first and second members to maintain a minimum distance between the first and second members and/or substantially center the one of the first and second members within the other. | 05-16-2013 |
20140042868 | DEVICES AND METHODS FOR MAGNETIC POLE AND BACK IRON RETENTION IN ELECTROMAGNETIC MACHINES - In some embodiments, an electromagnetic machine includes a rotor element configured for movement relative to a stator. The rotor element includes a support member, a backing member, and a magnetic pole assembly. The support member includes a first coupling portion. The backing member is formed, at least in part, from a ferromagnetic material and the magnetic pole assembly is configured to be coupled to the backing member. The magnetic pole assembly and/or the backing member include a second coupling portion configured to removably couple the backing member and the magnetic pole assembly collectively to the first coupling portion of the support member. | 02-13-2014 |
20140152136 | DEVICES AND METHODS FOR MAGNETIC POLE RETENTION IN ELECTROMAGNETIC MACHINES - In some embodiments, an electromagnetic machine includes a rotor element configured for movement relative to a stator. The rotor element includes a magnetic support, a magnetic pole assembly, and a retainer. The magnetic support is formed, at least in part, from a ferromagnetic material and is configured to be coupled to the magnetic support. The retainer is coupled to both the magnetic support and the magnetic pole assembly. The retainer is further configured to be in a first state during a first time period and a second state during a second time period, after the first time period, such that during the second time period, the coupling of the magnetic pole assembly to the magnetic support is maintained. | 06-05-2014 |