Kuersten
R. Scott Kuersten, Fitchburg, WI US
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20150099268 | AMPLIFICATION AND DETECTION OF RIBONUCLEIC ACIDS - Compositions, methods, and kits for detecting one or more species of RNA molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the RNA molecule using a polypeptide comprising double-strand specific RNA ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the reverse transcription product are removed. Primers are added and amplified products are generated. In certain embodiments, the sequence of at least part of at least one species of amplified product is determined and at least part of the corresponding RNA molecule is determined. In some embodiments, at least some of the amplified product species are detected, directly or indirectly, allowing the presence and/or quantity of the RNA molecule of interest to be determined. | 04-09-2015 |
20150147759 | Chimeric Oligonucleotides for Ligation-Enhanced Nucleic Acid Detection, Methods and Compositions Therefor - Ligation-enhanced nucleic acid detection assay embodiments for detection of RNA or DNA are described. The assay embodiments rely on ligation of chimeric oligonucleotide probes to generate a template for amplification and detection. The assay embodiments are substantially independent of the fidelity of a polymerase for copying compromised nucleic acid. Very little background amplification is observed and as few as 1000 copies of target nucleic acid can be detected. Method embodiments are particularly adept for detection of RNA from compromised samples such as formalin-fixed and paraffin-embedded samples. Heavily degraded and cross-linked nucleic acids of compromised samples, in which classic quantitative real time PCR assays typically fail to adequately amplify signal, can be reliably detected and quantified. | 05-28-2015 |
Scott Kuersten, Fitchburg, WI US
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20110104785 | METHODS AND KITS FOR 3'-END-TAGGING OF RNA - The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5′-activated, 3′-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides. | 05-05-2011 |
20140004569 | USE OF TEMPLATE SWITCHING FOR DNA SYNTHESIS | 01-02-2014 |
Scott Kuersten, Madison, WI US
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20140179562 | METHODS AND KITS FOR 3'-END-TAGGING OF RNA - The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5′-activated, 3′-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides. | 06-26-2014 |
20150291942 | MODIFIED TRANSPOSASES FOR IMPROVED INSERTION SEQUENCE BIAS AND INCREASED DNA INPUT TOLERANCE - Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same. | 10-15-2015 |
Uwe Kuersten, Karlsruhe DE
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20150339749 | CUSTOMER-SPECIFIC SMART INNOVATION DISCOVERY SERVICE - The present disclosure describes methods, systems, and computer program products for innovation discovery. One computer-implemented method includes receiving a request from a customer for usage data, the request received at a request interface for a service marketplace, upon a determination that usage data exists for the customer, initiating a request for usage data, routing the request for usage data though an external and internal process integration server system interface to a business information system, extracting usage data for the customer from the business information system, and return usage data to the requesting customer. | 11-26-2015 |