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Kubista
Daniel F. Kubista, Pine Island, MN US
| Patent application number | Description | Published |
|---|---|---|
| 20100052357 | Automated cover system for vehicle-mounted containers - A self-contained, vehicle-mounted system for operating a retractable cover with respect to proximate open top containers transported by the vehicle to cover and uncover the containers. The system includes all of the items necessary to accomplish the covering and uncovering of a container and needs only to be mounted on the vehicle and connected to a source of electric power on the vehicle. | 03-04-2010 |
David J. Kubista, Nampa, ID US
| Patent application number | Description | Published |
|---|---|---|
| 20100282164 | METHODS AND SYSTEMS FOR CONTROLLING TEMPERATURE DURING MICROFEATURE WORKPIECE PROCESSING, E.G., CVD DEPOSITION - The present disclosure provides methods and systems for controlling temperature. The method has particular utility in connection with controlling temperature in a deposition process, e.g., in depositing a heat-reflective material via CVD. One exemplary embodiment provides a method that involves monitoring a first temperature outside the deposition chamber and a second temperature inside the deposition chamber. An internal temperature in the deposition chamber can be increased in accordance with a ramp profile by (a) comparing a control temperature to a target temperature, and (b) selectively delivering heat to the deposition chamber in response to a result of the comparison. The target temperature may be determined in accordance with the ramp profile, but the control temperature in one implementation alternates between the first temperature and the second temperature. | 11-11-2010 |
| 20110163416 | METHODS FOR FORMING SMALL-SCALE CAPACITOR STRUCTURES - The present disclosure provides small scale capacitors (e.g., DRAM capacitors) and methods of forming such capacitors. One exemplary implementation provides a method of fabricating a capacitor that includes sequentially forming a first electrode, a dielectric layer, and a second electrode. At least one of the electrodes may be formed by a) reacting two precursors to deposit a first conductive layer at a first deposition rate, and b) depositing a second conductive layer at a second, lower deposition rate by depositing a precursor layer of one precursor at least one monolayer thick and exposing that precursor layer to another precursor to form a nanolayer reaction product. The second conductive layer may be in contact with the dielectric layer and have a thickness of no greater than about 50 Å. | 07-07-2011 |
Michael Kubista, Moelndal SE
| Patent application number | Description | Published |
|---|---|---|
| 20100216194 | SINGLE-CELL MRNA QUANTIFICATION WITH REAL-TIME RT-PCR - The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M guanidine thiocyanate, b) diluting the sample to an extend such that guanidine thiocyanate is present in a concentration of about 30 to 50 mM, c) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of a thermocycling protocol and monitoring amplification of the first strand cDNA in real time. | 08-26-2010 |
| 20110111463 | LYSIS AND REVERSE TRANSCRIPTION FOR MRNA QUANTIFICATION - The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA including the steps of (i) cultivation of a population of adherent cells in a cell culture vessel (ii) lysis of the population of adherent cells which is supposed to contain the target RNA in the sample vessel with a lysis buffer comprising between 0.05 M and 1 M of a chaotropic agent (iii) adding reagents to the sample vessel which are necessary to perform a reverse transcription reaction such that the the chaotropic agent is present in a concentration of about 10 to 60 mM in the sample vessel, and reverse transcribing the target RNA and (iv) amplifying the first strand cDNA by means of subjecting the sample to multiple cycles of a thermocycling protocol. | 05-12-2011 |
| 20110136180 | LYSIS AND REVERSE TRANSCRIPTION FOR MRNA QUANTIFICATION - The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M Guanidine Thiocyanate, b) diluting the sample to an extend such that Guanidine Thiocyanate is present in a concentration of about 30 to 50 mM, c) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of a thermocycling protocol and monitoring amplification of the first strand cDNA in real time, characterized in that steps a) to c) are done in the same vessel. | 06-09-2011 |
Mikael Kubista, Molnlycke SE
| Patent application number | Description | Published |
|---|---|---|
| 20080280295 | Use of Panel of Pairs of Primers Complementary to Reporter Genes of Cell Differentiation - The present invention to a panel comprising at least two pairs of primers that are complementary to at least two different reporter genes, the expression of which are i) either up- or down-regulated upon cell differentiation, and ii) display a similar expression profile in at least two different cell lines of the same kind of cells. The cells may be blastocyst-derived stem (BS) cells or human blastocyst-derived stem (hBS) cells. Furthermore, the present invention relates to the use of a calculated expression index for quantifying and evaluating the expression of the reporter genes, which for example can be used for assessing the state of differentiation of a cell population, such as, e.g. a hBS cell population. | 11-13-2008 |