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Kruckeberg
Arthur L. Kruckeberg, Shoreline, WA US
| Patent application number | Description | Published |
|---|---|---|
| 20080206813 | Means and methods for regulating gene expression - Described are means and methods for regulating gene expression and production of proteinaceous molecules. Described are methods for producing a proteinaceous molecule in a cell comprising selecting a cell for its suitability for producing the proteinaceous molecule, providing a nucleic acid encoding the proteinaceous molecule with a nucleic acid comprising a STAR (STabilizing Anti-Repression) sequence, expressing the resulting nucleic acid in the cell and collecting the proteinaceous molecule. Providing at least one STAR sequence to a nucleic acid encoding a proteinaceous molecule enhances production (yield) of the proteinaceous molecule by the host cell, increases the proportion of host cells with acceptable expression levels, and/or increases the stability of a gene expression level. | 08-28-2008 |
Arthur Leo Kruckeberg, Shoreline, WA US
| Patent application number | Description | Published |
|---|---|---|
| 20080227151 | Method for simultaneous production of multiple proteins; vectors and cells for use therein - Described is the production of proteins in a host cell. Even more specifically, described are methods for improving expression of two or more proteins in a cells or host cell. The methods are suited for production of, for example, recombinant antibodies that can be used in pharmaceutical preparations or as diagnostic tools. In certain embodiments, provided are methods for obtaining a cell that expresses two or more proteins comprising providing the cell with two or more protein expression units encoding two or more proteins, characterized in that at least two of the protein expression units comprise at least one STAR sequence. | 09-18-2008 |
| 20080227199 | Method for simultaneous production of multiple proteins; vectors and cells for use therein - Described is the production of proteins in a host cell. More specifically, described are methods for improving expression of two or more proteins in a cell or host cell. The methods are suited for production of, for example, recombinant antibodies that can be used in pharmaceutical preparations or as diagnostic tools. In one embodiment, provided is a method for obtaining a cell that expresses two or more proteins comprising providing the cell with two or more protein expression units encoding two or more proteins, characterized in that at least two of the protein expression units comprise at least one STAR sequence. | 09-18-2008 |
Arthur Leo Kruckeberg, Wilmington, DE US
| Patent application number | Description | Published |
|---|---|---|
| 20120015416 | EXPRESSION OF HEXOSE KINASE IN RECOMBINANT HOST CELLS - The invention relates to a recombinant host cell having (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity; (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and optionally (c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity. Additionally, the invention relates to methods of making and using such recombinant host cells including, for example, methods of increasing glucose consumption, methods of improving redox balance, and/or methods of increasing the production of a product of a pyruvate-utilizing pathway. | 01-19-2012 |
Keith A. Kruckeberg, Owatonna, MN US
| Patent application number | Description | Published |
|---|---|---|
| 20090283530 | Non-Metallic Auxiliary Tank System for a Vehicle - An on-board auxiliary split tank system for supplying makeup water and chemical additives to a transit concrete mixing vehicle is disclosed which includes a generally cylindrical water tank designed for generally horizontal deployment having a shaped recess therein and an additive tank configured to nest in said recess of said water tank and which, when nested in said recess, generally completes said cylindrical shape. The water tank and additive tank are formed from a non-metallic material comprising a polymeric component. | 11-19-2009 |