Patent application number | Description | Published |
20090117609 | USE OF TETRACYSTEINE TAGS IN FLUORESCENCE-ACTIVATED CELL SORTING ANALYSIS OF PROKARYOTIC CELLS PRODUCING PEPTIDES OR PROTEINS - A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected. | 05-07-2009 |
20100159513 | GENES THAT INCREASE PEPTIDE PRODUCTION - Several endogenous genes have been identified in | 06-24-2010 |
20100227361 | HOST CELL MODIFICATIONS THAT IMPROVE PEPTIDE PRODUCTION AND DOWNSTREAM PROCESSING - Disrupting the expression of endogenous | 09-09-2010 |
20120122168 | RECOMBINANT ESCHERICHIA COLI HAVING ENHANCED ACETYL-COENZYME A SYNTHETASE ACTIVITY FOR PRODUCING GLYEROL AND GLYCEROL-DERIVED PRODUCTS - Recombinant | 05-17-2012 |
20120214202 | RECOMBINANT HOST CELLS HAVING AN INCREASE IN BUOYANT DENSITY - Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs. | 08-23-2012 |
20130045518 | VARIANT SUCROSE TRANSPORTER POLYPEPTIDES THAT ENABLE FASTER SUCROSE UTILIZATION IN BACTERIA - Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria are described. Additionally, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided. | 02-21-2013 |
20130045519 | RECOMBINANT BACTERIA HAVING IMPROVED SUCROSE UTILIZATION - Recombinant bacteria having an improved ability to utilize sucrose are provided. These recombinant bacteria have nucleotide sequences encoding sucrose utilization polypeptides integrated into their genome between the yihP gene or its homolog and the yihO gene or its homolog. Additionally, methods of utilizing the recombinant bacteria to produce products such as glycerol and glycerol-derived products are provided. | 02-21-2013 |
20130230893 | RECOMBINANT BACTERIA COMPRISING NOVEL SUCROSE TRANSPORTERS - Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described. | 09-05-2013 |
20140093927 | Variant Sucrose Transporter Polypeptides That Enable Faster Sucrose Utilization In Bacteria - Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria are described. Additionally, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided. | 04-03-2014 |
20150064755 | RECOMBINANT BACTERIA COMPRISING NOVEL SUCROSE TRANSPORTERS - Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described. | 03-05-2015 |