| Patent application number | Description | Published |
|---|
| 20090093371 | MEMBRANE ARRAYS AND METHODS OF MANUFACTURE - The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane. | 04-09-2009 |
| 20090098645 | Cell culture article and methods thereof - Disclosed is a cell culture article including: a substrate; a tie-layer attached to at least the substrate; and a bio-compatible layer attached to at least the tie layer, the bio-compatible layer having been obtained from surface oxidation of a polymer layer. Also disclosed are methods for making the cell culture article and methods for performing an assay of a ligand with the article. | 04-16-2009 |
| 20090131263 | Normalization methods for G-protein coupled receptor membrane array - Reference membrane components are either pre-labeled or labeled during assays for purposes of normalizing signals associated with binding or functional assays employing G-protein coupled receptor microarrays. A reference component may be included in a membrane in which the target GPCR is embedded or may be present in another membrane printed in conjunction with the target membrane on a microspot. Or, a GPCR microarray may be pre-labeled by incorporating a label on an exposed substrate in a defect in the printed microspot. | 05-21-2009 |
| 20090142790 | Label Free Biosensors and Cells - Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in high throughput methods and can be multiplexed. | 06-04-2009 |
| 20090186776 | Microcolumn-platform based array for high-throughput analysis - A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity. | 07-23-2009 |
| 20090215650 | SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF - The present invention provides a method for preparing a physically stable array of biological membranes, including membrane proteins, on a surface, and the resultant article of manufacture. The method comprises providing a substrate; creating either a polar surface or reactive surface by coating the substrate with a material that either: (1) enhances the stability of lipid spots during withdrawing through a water/air interface and washing and drying protocols; or (2) gives rise to minimal non-specific binding of a labeled target to a background surface, and high specific binding to a probe receptor in said membrane array, or (3) both; and depositing an array of biological-membrane microspots on the substrate. The method may further comprise applying a reagent that includes a soluable protein to stabilize the biological membranes on the surface. Also provided is an article having biological-membrane microspots that are associated in a stable fashion with a substrate surface embodying these properties. | 08-27-2009 |
| 20090275074 | System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors - The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells. | 11-05-2009 |
| 20090325211 | System and method for dual-detection of a cellular response - A system and method as defined herein for dual-detection of evanescent-wave label-free light and evanescent-wave excited-fluorescent label-emitted light in an optical biosensor. | 12-31-2009 |
| 20100129856 | Methods and Devices for Cell Signaling Under Pulse Stimulation - The disclosure provides a device for measuring cellular responses under controlled environments. The disclosure also provides an array of devices for measuring cellular responses under controlled environments. The disclosure also provides methods to study cell signaling using the devices. By using microfluidics, the disclosure enables study of cell signaling under well-defined environment conditions. Such capability can be used for differentiating long-acting ligands from short-acting ligands, for determining the kinetics of receptor resensitization and functional recovery of receptor signaling, and for differentiating the sensitivity of a cell type to laminar flow-induced stress force. Through combination with conventional static label-free cell assays, the full spectrum of a drug compound can be studied in details, thus creating a complete representation of its pharmacology acting on living cells. | 05-27-2010 |
| 20100130725 | METHODS FOR CHARACTERIZING MOLECULES - Drug discovery is a complex undertaking facing many challenges, not the least of which is a high attrition rate as many promising candidates prove ineffective or toxic in the clinic owing to a poor understanding of the diseases, and thus the biological systems, they target. Therefore, it is broadly agreed that to increase the productivity of drug discovery one needs a far deeper understanding of the molecular mechanisms of diseases, taking into account the full biological context of the drug target and moving beyond individual genes and proteins. The present methods rely on the use of label-free cellular assays, particularly the DMR index, to systematically display the mode of actions, the toxicity, and the target(s) and pathway(s) of any molecules. | 05-27-2010 |
| 20100130736 | METHODS OF CREATING AN INDEX - Drug discovery is a complex undertaking facing many challenges, not the least of which is a high attrition rate as many promising candidates prove ineffective or toxic in the clinic owing to a poor understanding of the diseases, and thus the biological systems, they target. Therefore, it is broadly agreed that to increase the productivity of drug discovery one needs a far deeper understanding of the molecular mechanisms of diseases, taking into account the full biological context of the drug target and moving beyond individual genes and proteins. The present methods rely on the use of label-free cellular assays, particularly the DMR index, to systematically display the mode of actions, the toxicity, and the target(s) and pathway(s) of any molecules. | 05-27-2010 |
| 20100184626 | ARRAYS OF BIOLOGICAL MEMBRANES AND METHODS AND USE THEREOF - The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase. | 07-22-2010 |
| 20100297675 | SUBSTRATE AND METHOD FOR CULTURING BREAST CELLS - A cell culture article includes a porous substrate having a plurality of pores and a plurality of interstices in communication with the pores. At least some of the plurality of pores and interstices are sufficiently large for two or more mammary epithelial cells to cluster within the pores or interstices. Non-malignant mammary epithelial cells or breast cancer cells may not attach strongly to the substrate surface, which may encourage cell-cell interaction. In many cases, the article is desirably free of components of unknown origin. The articles may be capable of maintaining culture of malignant and non-malignant mammary epithelial cells and allowing for development of in vivo-like morphologies or characteristics of such cells. | 11-25-2010 |
| 20100323902 | Live-cell signals of pathogen intrusion and methods thereof - Disclosed is a system and method for measuring aspects of pathogen intrusion on a live-cell as defined herein. The system and method also provide a method to measure prophylaxis or remedial aspects of a therapeutic candidates in a live-cell or a live-cell model from pathogen intrusion. | 12-23-2010 |
| 20110008912 | Polymer-coated substrates for binding biomolecules and methods of making and using Thereof - Described herein are polymer-coated substrates for binding biomolecules and methods of making and using thereof. | 01-13-2011 |
