Patent application number | Description | Published |
20090013430 | Whisker-mediated transformation of plant cell aggregates and plant tissues and regeneration of plants thereof - Plant cell aggregates and plant tissues can be transformed by elongated, needle-like structures called “whiskers”. The process comprises the agitation of plant cell aggregates and plant tissues of the plant to be transformed in the presence of DNA and whiskers, whereby DNA uptake and integration thereof in facilitated. The process may be applicable to other plant cell aggregates and plant tissues which have not proven easily transformable by other techniques. | 01-08-2009 |
20090111188 | Optimized non-canonical zinc finger proteins - Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described. | 04-30-2009 |
20100311168 | NANOPARTICLE MEDIATED DELIVERY OF SEQUENCE SPECIFIC NUCLEASES - Provided are methods for introducing a sequence specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. | 12-09-2010 |
20110008833 | PROTEIN PRODUCTION IN PLANT CELLS AND ASSOCIATED METHODS AND COMPOSITIONS - The present invention relates to methods and compositions for the expression of protein. Embodiments of methods may comprise the cleavage and repair of a nucleotide sequence encoding a highly expressed protein leading to a reduction in the expression of the highly expressed protein. | 01-13-2011 |
20110167521 | Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis - The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided. | 07-07-2011 |
20110191877 | EXCISION OF TRANSGENES IN GENETICALLY MODIFIED ORGANISMS - A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included. | 08-04-2011 |
20120173153 | DATA ANALYSIS OF DNA SEQUENCES - Systems and methods for data analysis are provided. In one embodiment, a method may be provided for analysis comprising electronically receiving sequence data related to a plurality of sequences and a reference sequence, associating the sequence data with one of at least two groups, identifying a plurality of high quality read sequences from among the plurality of sequences, extracting a plurality of unique read sequences from the plurality of high quality read sequences, and aligning the plurality of unique read sequences against the reference sequence data corresponding to a reference sample. The method may further identify mutations in a targeted location, display the targeted mutations, and prioritize the technologies that caused the mutations according to their efficiency. In one example, the systems and methods are used to characterize the activity of several ZFN candidates. | 07-05-2012 |
20140173783 | PRECISION GENE TARGETING TO A PARTICULAR LOCUS IN MAIZE - The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed. | 06-19-2014 |
20140304853 | METHODS AND COMPOSITIONS FOR INTEGRATION OF AN EXOGENOUS SEQUENCE WITHIN THE GENOME OF PLANTS - Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes. | 10-09-2014 |