| Patent application number | Description | Published |
|---|
| 20090068643 | Dual Function Primers for Amplifying DNA and Methods of Use - The present invention provides novel nucleotide compositions that enable the detection of DNA synthesis products and methods for use thereof. In one embodiment, the method can be used in PCR and allows the progress of the reaction to be monitored as it occurs. In one embodiment, the invention employs at least one fluorescence-quenched oligonucleotide that can prime DNA extension reactions. In a second embodiment, the invention employs at least one fluorescence-quenched oligonucleotide that can function as a template for DNA extension reactions. In both embodiments, the oligonucleotide also functions as a probe for detecting the progress of successive extension reaction cycles. Signal detection is dependent upon DNA synthesis and can occur with or without probe cleavage. | 03-12-2009 |
| 20090118482 | COMPOUNDS AND METHODS FOR LABELING OLIGONUCLEOTIDES - The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below. | 05-07-2009 |
| 20090299041 | COMPOUNDS AND METHODS FOR LABELING OLIGONUCLEOTIDES - The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below. | 12-03-2009 |
| 20100075383 | METHODS FOR AMPLIFYING POLYMERIC NUCLEIC ACIDS - The invention provides compositions and methods for amplifying nucleic acid polymer sequences in a high complexity nucleic acid sample. The unique compositions of the invention include a primer set composed of a mixture of two types of primers for DNA synthesis. For extension in one direction, the primers all contain modifications that destroy their ability to serve as templates that can be copied by DNA polymerases. For extension in the opposite direction the set includes at least one primer that can serve as a template and be replicated by DNA polymerases throughout its length. The method can be carried out by mixing the nucleic acid polymer sequence of interest with the set of DNA synthesis primers in an amplification reaction mixture. The reaction mixture is then subjected to temperature cycling analogous to the temperature cycling in PCR reactions. At least one primer in the primer set hybridizes to the nucleic acid polymer. It is preferred that the non-replicable primer hybridizes to the nucleic acid polymer and is extended to produce an extension product that contains sequence from the nucleic acid polymer to which the replicable primer then hybridizes. Of course, if the nucleic acid polymer is double stranded, both the replicable and nonreplicable primers will hybridize and be extended by DNA polymerase. | 03-25-2010 |
| 20100076181 | COMPOUNDS AND METHODS FOR LABELING OLIGONUCLEOTIDES - The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below. | 03-25-2010 |
| 20100298554 | COMPOUNDS AND METHODS FOR LABELING OLIGONUCLEOTIDES - A compound having the general formula shown below: | 11-25-2010 |
| 20110236898 | METHODS FOR ENHANCING NUCLEIC ACID HYBRIDIZATION - A composition comprising an oligonucleotide having the structure 5′-Y | 09-29-2011 |
| 20110319606 | COMPOUNDS AND METHODS FOR LABELING OLIGONUCLEOTIDES - A compound having the general formula shown below: | 12-29-2011 |
