Patent application number | Description | Published |
20090029414 | CELL-FREE SYNTHESIS OF MEMBRANE BOUND POLYPEPTIDES - Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of membrane- associated polypeptides. | 01-29-2009 |
20090042244 | PROTEIN EXPRESSION YIELD ENHANCEMENT IN CELL-FREE PROTEIN SYNTHESIS SYSTEMS BY ADDITION OF ANTIFOAM AGENTS - Compositions and methods are provided for the in vitro synthesis of biological molecules in reaction mixtures comprising anti-foam agents. The reaction mix comprising antifoam agent may be a scaled up reaction, e.g. in reaction volume greater than at least about 15 ul. Reactions may be performed in various reactors, as known in the art, which include stirred reactors, bubble-column reactors; and the like. | 02-12-2009 |
20090317861 | CELL-FREE SYNTHESIS OF VIRUS LIKE PARTICLES - Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of virus like particles. | 12-24-2009 |
20100063258 | FUSION PROTEIN CONSTRUCTS - Polypeptide linkers with defined tertiary structures, usually of defined alpha helical structure, are used to join two domains in a fusion protein. In one embodiment of the invention, a method is provided for the cell-free synthesis of the fusion protein. | 03-11-2010 |
20100093024 | CELL-FREE SYNTHESIS OF PROTEINS CONTAINING UNNATURAL AMINO ACIDS - Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of polypeptides containing unnatural amino acids at one or more specified residues of the polypeptide. | 04-15-2010 |
20100183655 | Immunogenic Protein Constructs - Bacterial immunity proteins are utilized to increase immune response to an antigen of interest. | 07-22-2010 |
20110129438 | IMMUNOGENIC PROTEIN CONSTRUCTS - Bacterial immunity proteins are utilized to increase immune response to an antigen of interest. | 06-02-2011 |
20120208232 | Cell-Free Synthesis of Active Reprogramming Transcription Factors - Compositions and methods are provided for the cell-free synthesis of active reprogramming factor polypeptides. The reprogramming factors may be synthesized as fusion proteins comprising a permeant domain, such as polyarginme. The cell free-synthesis may be conducted at about 25 C in a bacterial cell extract from genetically alterd cells having decreased endogenous protease activity Further, the proteins may comprise a fusion partner which enhances solubility and may be refolded on a column. | 08-16-2012 |
20130065294 | Cell-Free Synthesis of Membrane Bound Polypeptides - Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of membrane-associated polypeptides. | 03-14-2013 |
20130316397 | Cell-Free Polypeptide Synthesis - Methods, microbial strains and reaction mixtures for cell-free synthesis of polypeptides are provided. The methods of the invention utilize a reaction mixture comprising microbial cell extracts that are modified in the protein component relative to an extract from a native cell. The modification may be one or both of (i) increased levels of proteins that increase synthetic yield; and (ii) decreased levels of proteins that decrease synthetic yield. The modification may result from a genetic modification of the microbial cell, or from ex vivo supplementation or depletion of an extract. | 11-28-2013 |
20130323281 | ORDERED FLAGELLIN ARRAY AS AN IMMUNOSTIMULANT - Bacterial flagellin protein is modified to improve adjuvant activity. | 12-05-2013 |