Hubbuch
Juergen Hubbuch, Karlsruhe DE
Patent application number | Description | Published |
---|---|---|
20100242634 | Method for depositing samples in modules and an adapter - The invention relates to a method for depositing samples in modules, in particular, in kits, in addition to an adapter. The inventive adapter ( | 09-30-2010 |
Jürgen Hubbuch, Köln DE
Patent application number | Description | Published |
---|---|---|
20100036109 | METHOD FOR SELECTIVELY AND REVERSIBLY ADSORBING NUCLEIC ACIDS ON A CARRIER MATERIAL - The invention relates to a process for selective, reversible adsorption of nucleic acids to a support material, which process is characterized in that (a) a nucleic acid-containing mixture having a cosmotropic salt content of at least 1 mole is contacted with said support material, whereupon the nucleic acid(s) is (are) adsorbed from said nucleic acid-containing mixture to said support material, and (b) the nucleic acid(s) bound to the support material is (are) detached from the support material by means of an aqueous solution having a salt content of no more than 1 mole. | 02-11-2010 |
Jürgen Hubbuch, Karlsruhe DE
Patent application number | Description | Published |
---|---|---|
20100009434 | Method for the Selective Enrichment of Double-Stranded Dna from Nucleic Acid Mixtures - The invention relates to a method for stripping undesired nucleic acid components from double-stranded DNA, in particular, super-coiled plasmid DNA. The method according to the invention is characterised by the steps: (a) provision of a mixture containing completely and/or partly double-stranded nucleic acids and optionally single-stranded nucleic acids; (b) resuspension of the mixture from step (a) in an aqueous, low-molarity buffer system with low ionic strength and low buffer effect; (c) adjusting conditions in the mixture from step (b), under which the completely and/or partly double-stranded nucleic acids are denatured; (d) further addition of buffer and a polymer component to the mixture from step (c); (e) incubation of the mixture from step (d) for a time which is sufficient for the formation of an aqueous two-phase system with an upper and lower phase; and (f) removal of the upper phase containing the single-strand nucleic acid and collection of the double-strand nucleic acid from the lower phase. | 01-14-2010 |
Jürgen Hubbuch, Köln DE
Patent application number | Description | Published |
---|---|---|
20100036109 | METHOD FOR SELECTIVELY AND REVERSIBLY ADSORBING NUCLEIC ACIDS ON A CARRIER MATERIAL - The invention relates to a process for selective, reversible adsorption of nucleic acids to a support material, which process is characterized in that (a) a nucleic acid-containing mixture having a cosmotropic salt content of at least 1 mole is contacted with said support material, whereupon the nucleic acid(s) is (are) adsorbed from said nucleic acid-containing mixture to said support material, and (b) the nucleic acid(s) bound to the support material is (are) detached from the support material by means of an aqueous solution having a salt content of no more than 1 mole. | 02-11-2010 |
Jürgen Hubbuch, Karlsruhe DE
Patent application number | Description | Published |
---|---|---|
20100009434 | Method for the Selective Enrichment of Double-Stranded Dna from Nucleic Acid Mixtures - The invention relates to a method for stripping undesired nucleic acid components from double-stranded DNA, in particular, super-coiled plasmid DNA. The method according to the invention is characterised by the steps: (a) provision of a mixture containing completely and/or partly double-stranded nucleic acids and optionally single-stranded nucleic acids; (b) resuspension of the mixture from step (a) in an aqueous, low-molarity buffer system with low ionic strength and low buffer effect; (c) adjusting conditions in the mixture from step (b), under which the completely and/or partly double-stranded nucleic acids are denatured; (d) further addition of buffer and a polymer component to the mixture from step (c); (e) incubation of the mixture from step (d) for a time which is sufficient for the formation of an aqueous two-phase system with an upper and lower phase; and (f) removal of the upper phase containing the single-strand nucleic acid and collection of the double-strand nucleic acid from the lower phase. | 01-14-2010 |