Patent application number | Description | Published |
20080268497 | TREATMENT SOLUTION, METHOD FOR PRETREATMENT, METHOD FOR ACQUIRING INFORMATION AND METHOD FOR DETECTION - The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens μm and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis. | 10-30-2008 |
20080277576 | TIME-OF-FLIGHT SECONDARY ION MASS SPECTROMETER - A time-of-flight secondary ion mass spectrometer comprises an ion source which generates cluster ions each comprised of two or more atoms, a pulsing mechanism which pulses the cluster ions, a selecting mechanism which selects ions having a specific mass number from the pulsed cluster ions and passes the selected ions in an ON state of the selecting mechanism, and, passes the pulsed cluster ions without the selecting in an OFF state of the selecting mechanism, and a time-of-flight mass spectrometric unit which measures a mass spectrum of secondary ions generated from a sample using a difference in time of flight when the sample is irradiated with the ions passed through the selecting mechanism. | 11-13-2008 |
20080278706 | METHOD FOR PRE-TREATING SPECIMEN AND METHOD FOR ANALYZING SPECIMEN - A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method. | 11-13-2008 |
20080290268 | INFORMATION OBTAINING METHOD - An information obtaining method for obtaining information about a mass of a component of an analyte using a time of flight mass spectrometer and obtaining information about a distribution state of the component based on the obtained information about the mass includes the step of: (1) adding a self-reactive substance to the analyte on a base to facilitate ionization of the component; (2) irradiating the analyte with a primary beam in the presence of the self-reactive substance, thereby ionizing the components and allowing resulting ions to fly; (3) obtaining information about mass of the flying ions using the time of flight mass spectrometer; and (4) obtaining information about the distribution state of the component on the base based on the information about the mass. | 11-27-2008 |
20090026364 | METHOD OF MEASURING TARGET OBJECT IN A SAMPLE USING MASS SPECTROMETRY - In-plane distribution of a target object contained in a sample is measured. The sample dispersedly placed on a substrate is treated to promote ionization of the target object. Then, the mass and flying amount of an ion containing the target object or a component thereof is determined by irradiating an ion beam to the sample and performing time-of-flight secondary ion mass spectrometry of the ion that flies from a portion in the sample where the ion beam is irradiated, and the in-plane distribution of the target object is determined from the mass and flying amount data obtained at plural portions by scanning the beam on the sample plane. The step of treating the sample to promote ionization of the target object includes contacting an aqueous solution of an acid that does not crystallize at ordinary temperature with the sample. A high spatial resolution two-dimensional image can be obtained. | 01-29-2009 |
20090236767 | METHOD OF FEEDING MOLTEN RESIN AND APPARATUS FOR FEEDING MOLTEN RESIN - An apparatus for feeding a molten resin is operated for extended periods of time maintaining stability without permitting volatile components of the molten resin to adhere on the surfaces of conveyer means. An extruder in the apparatus for feeding the molten resin to the compression-forming machine has an extrusion unit which discharges a molten resin through an extrusion opening of an extrusion nozzle. An air injection nozzle for injecting the cooling gas is provided along the outer circumference of the extrusion nozzle, and the cooling gas is blown onto the surface of the molten resin extruded from the extrusion opening to cool the surface of the molten resin. | 09-24-2009 |
20090266982 | MASS SPECTROMETRY SUBSTRATE AND MASS SPECTROMETRY METHOD - A mass spectrometry method that makes it possible to perform high-sensitivity detection of a desorbed/ionized object substance to be measured in mass spectrometry in which the object substance to be measured is desorbed and ionized. The method includes placing at least an ionizing agent having two or more functional groups represented by Formula (1) below in a molecule: | 10-29-2009 |
20090308141 | METHOD FOR PREPARING TEST SPECIMEN - A test specimen is provided which has one or more chemical substances fixed to prescribed plural independent positions on a substrate, and the quantities of the chemical substances fixed at the respective prescribed positions are the total of integer multiples of existence quantity units defined for the respective chemical substances in the range from 1 amol to 1 nmol (excluding the case in which the total quantity is zero). | 12-17-2009 |
20100227308 | INFORMATION ACQUISITION METHOD, INFORMATION ACQUISITION APPARATUS AND DISEASE DIAGNOSIS METHOD - An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry. | 09-09-2010 |
20110171363 | SCREENING METHOD FOR TEST SPECIMEN - A test specimen that has one or more chemical substances fixed to prescribed plural independent positions on a substrate, and the quantities of the chemical substances fixed at the respective prescribed positions are the total of integer multiples of existence quantity units defined for the respective chemical substances in the range from 1 amol to 1 nmol (excluding the case in which the total quantity is zero). | 07-14-2011 |
20110248156 | INFORMATION ACQUIRING APPARATUS AND INFORMATION ACQUIRING METHOD FOR ACQUIRING MASS-RELATED INFORMATION - Target molecules in a sample can be detected at an improved sensitivity by means of a mass spectrometer. A sample with or without a matrix is placed on a substrate and irradiated with a converged and pulsed primary beam selected from an ion beam, a neutral particle beam or a laser beam. Secondary ions and neutral molecules are emitted along with protons from the irradiated point of the sample as an electric field is applied between the substrate and an extraction electrode disposed above the substrate. A proton-control electrode is arranged in axial symmetry with the trajectory of the primary beam. A voltage is applied thereto so that the generated electric field decelerates the flying protons to raise their adhering efficiency to the flying neutral molecules. | 10-13-2011 |
20110315871 | METHOD OF FORMING MASS IMAGE - An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level. | 12-29-2011 |
20120181421 | INFORMATION ACQUISITION METHOD - Provided is a method that achieves both of soft ionization and high ionization efficiency of a substance to be analyzed at each measurement site without impairing a two-dimensional distribution state of the substance to be analyzed in desorption ionization mass spectrometry of a substance to be measured. By applying, to a substance to be analyzed, an ionization assisting reagent including an organic acid including a functional group represented by —(CF | 07-19-2012 |
20120298859 | METHOD AND APPARATUS FOR REDUCING NOISE IN MASS SIGNAL - A more effective noise reduction method is provided. In the method, when mass spectrum information having a spatial distribution is processed, the whole data is taken as three-dimensional data (positional information is stored in an xy plane, and spectral information is stored along a z-axis direction), and three-dimensional wavelet noise reduction is performed by applying preferable basis functions to a spectral direction and a peak distribution direction (in-plane direction). | 11-29-2012 |
20120301968 | MASS SPECTROMETRY METHOD - The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry. | 11-29-2012 |
20130158882 | METHOD OF FORMING MASS IMAGE - An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level. | 06-20-2013 |
20130181368 | METHOD AND DEVICE FOR FORCIBLY INSERTING DROP INTO COMPRESSION MOLDING MACHINE, AND MOLDING DIE FOLLOW-UP TYPE METHOD AND DEVICE FOR SUPPLYING DROP - A method and device for continuously supplying molten synthetic resin drops into female moldings which are rotatingly movable for manufacturing moldings, wherein synthetic resin in molten condition extruded from an extrusion opening is cut by a cutter attached to a holding mechanism to form the molten resin into drops in a determined quantity, the drops are held and conveyed by the holding mechanism, and the drops are forcibly inserted and supplied into the concaves of the female moldings. At that time the holding mechanism on the rotary-and movable type drop supply is made to approach the rotating molding die and the rotation path of the holding mechanism is made to overlap with that of the molding die within a determined area to make the movement of the holding mechanisms follow that of the molding die. | 07-18-2013 |
20130203105 | METHOD FOR PRE-TREATING SPECIMEN AND METHOD FOR ANALYZING SPECIMEN - A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method. | 08-08-2013 |
20130320204 | SAMPLE ANALYSIS METHOD AND ANALYZER - Provided is a sample analysis method of irradiating a sample with a primary ion beam to analyze a secondary ion emitted from the sample by mass spectrometry, the sample analysis method including the steps of cooling a sample placed in a chamber; forming an ice layer on a surface of the cooled sample by discharging one of water and an aqueous solution to the chamber; and irradiating the surface of the sample with the primary ion beam with the ice layer being formed thereon, wherein an amount of the water forming the ice | 12-05-2013 |
20140087408 | INFORMATION ACQUISITION METHOD - The present invention relates to an information acquisition method of acquiring information of distribution of a protein or peptide in a sample based on mass information obtained by mass spectrometry of the protein or peptide. The method includes mass spectrometry of a definite region of the sample after limited proteolysis of the protein or peptide and acquisition of information relating to distribution using an ion peak that has a two-dimensional intensity distribution having a Pearson product-moment correlation coefficient of 0.5 to more and 1.0 or less in the definite region against the two-dimensional intensity distribution of the parent ion of the protein or the peptide subjected to the limited proteolysis and has a peak intensity ratio of larger than 1.0 against the peak intensity of the integrated spectrum of the parent ion in the definite region, wherein the m/z of the ion peak is greater than 500. | 03-27-2014 |
20140354987 | TEST APPARATUS AND METHOD OF OBSERVING BIOPSY SPECIMEN SAMPLED BY USING TEST APPARATUS - A test apparatus includes a biopsy needle for sampling a biopsy specimen. The biopsy needle includes a specimen holder that holds the sampled biopsy specimen, and an optical window disposed in the specimen holder and configured to allow optical detection. A biopsy specimen held by the specimen holder of the test apparatus is measured by using a third-order nonlinear Raman microscope. | 12-04-2014 |