Hiddessen
Amy Hiddessen, Pleasanton, CA US
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20140134623 | STABILIZED DROPLETS FOR CALIBRATION AND TESTING - Provided herein, are droplet mixture compositions and systems and methods for forming mixtures of droplets. The system may comprise two or more droplet generation units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The combined droplet populations can be mixed, heated, and collected for multiple uses, such as for use as calibration standards for instrument testing and analysis. | 05-15-2014 |
Amy Hiddessen, Dublin, CA US
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20140302503 | COMPOSITIONS, METHODS AND SYSTEMS FOR POLYMERASE CHAIN REACTION ASSAYS - The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection. | 10-09-2014 |
Amy L. Hiddessen, Hercules, CA US
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20140179544 | COMPOSITIONS, SYSTEMS AND METHODS FOR DROPLET FORMATION, SPACING AND DETECTION - The present disclosure provides assays and devices for forming, spacing, and/or detecting droplets. The droplets may be emulsions composed of two or more immiscible fluids. An emulsion can be a double emulsion, such as water-in-oil droplets that are present in a continuous aqueous phase. The double emulsion can be formed when the water-in-oil droplets are contacted with one or more streams of aqueous fluid(s). This disclosure also provides a variety of additives that can be added to the fluids. | 06-26-2014 |
Amy L. Hiddessen, Pleasanton, CA US
Patent application number | Description | Published |
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20140170665 | SYSTEMS AND METHODS FOR STABILIZING DROPLETS - This disclosure provides systems, devices, and methods for sample preparation and/or analysis comprising a droplet generator having a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel may meet at an intersection that receives a sample from the sample reservoir and a carrier fluid from the carrier fluid reservoir and generates one or more droplets that flow along a droplet channel to a droplet reservoir. An energy application member in thermal communication with the intersection, at least a portion of the droplet channel, the sample reservoir and/or the carrier fluid reservoir may provide energy to an individual droplet of the one or more droplets, such as at the intersection and/or as the droplet moves along the droplet channel to the droplet reservoir. | 06-19-2014 |