Patent application number | Description | Published |
20080218184 | NANOPORE PLATFORMS FOR ION CHANNEL RECORDINGS AND SINGLE MOLECULE DETECTION AND ANALYSIS - Chemical modification of a glass and fused silica nanopore surfaces results in surface properties that are ideal for localized bilayer formation over a nanopore and subsequent ion channel recording. With no surface modification, one may form a bilayer supported on the glass capillary extending across the nanopore orifice. Changing the surface properties from that of bare glass to a moderately hydrophobic surface produces a lipid monolayer above the glass and spontaneously yields a bilayer across the nanopore orifice, effectively corralling a single protein ion channel in the lipid bilayer region spanning nanopore orifice. The bilayer structure over the modified nanopore is such that current can only flow through the protein ion channel. The protein ion channel is able to diffuse in the bilayer above the pore opening, but cannot leave this area to enter the lipid monolayer. The bilayer formed across the nanopore orifice exhibits high electrical breakdown voltage, is stable to mechanical vibrations, and is long lived. Resistance through the protein channel can be measured electrically and is exploited for stochastic single-molecule detection. Protein ion channels can be inserted and removed from the bilayer by adjusting transmembrane pressure, and adapter molecules can be electrostatically trapped in the ion channel by applying high transmembrane voltages. | 09-11-2008 |
20090175765 | Surfaces Resistant to Non-Specific Protein Adsorption and Methods of Producing the Same - A method of preventing non-specific adsorption of proteins onto a surface can include providing a substrate that has a surface on which surface groups are attached. A solution can be applied to the surface that includes a protective reagent having a terminal functional group exhibiting a dipole moment. A monolayer comprising the protective reagent is assembled on the surface by reacting the protective reagent with the surface groups, thereby creating a protected surface. The protective reagent alone is sufficient to confer to the protected surface an increased resistance to adsorption of proteins. | 07-09-2009 |
20100025263 | NANOPORE PARTICLE ANALYZER, METHOD OF PREPARATION AND USE THEREOF - Provided are the preparation, characterization, and application of a nanopore membrane device. The nanopore device comprises a thin membrane prepared from glass, fused silica, ceramics or quartz, containing one or more nanopores ranging from about 2 nm to about 500 nm. The nanopore is prepared by a template method using sharpened metal wires and the size of the pore opening can be controlled during fabrication by an electrical feedback circuit. The nanopore device is particularly useful for counting and analyzing nanoparticles of radius less than 400 nm. | 02-04-2010 |
20100038243 | NANOPORE BASED ION-SELECTIVE ELECTRODES - Nanopore based ion-selective electrodes and methods of their manufacture as well as methods for their use are disclosed and described. The nanopore based ion-selective electrode can include a pore being present in a solid material and having a nanosize opening in the solid material, a metal conductor disposed inside the pore opposite the opening in the solid material, a reference electrode material contacting said metal conductor and disposed inside the pore, a conductive composition in contact with the reference electrode and disposed in the pore, and an ion-selective membrane. The ion-selective membrane can be configured to isolate the metal conductor, reference electrode material, and conductive composition together within the pore. | 02-18-2010 |
20100320094 | Nanopore Platforms for Ion Channel Recordings and Single Molecule Detection and Analysis - A nanopore device includes a membrane having a nanopore extending there through forming a channel from a first side of the membrane to a second side of the membrane. The surface of the channel and first side of the membrane are modified with a hydrophobic coating. A first lipid monolayer is deposited on the first side of the membrane, and a second lipid monolayer is deposited on the second side of the membrane, wherein the hydrophobic coating causes spontaneous generation of a lipid bilayer across the nanopore orifice. Sensing entities, such as a protein ion channel, can be inserted and removed from the bilayer by adjusting transmembrane pressure, and adapter molecules can be electrostatically trapped in the ion channel by applying high transmembrane voltages, while resistance or current flow through the sensing entity can be measured electrically. | 12-23-2010 |
20110168551 | Nanopore Electrode, Nanopore Membrane, Methods of Preparation and Surface Modification, and Use Thereof - Provided are fabrication, characterization and application of a nanodisk electrode, a nanopore electrode and a nanopore membrane. These three nanostructures share common fabrication steps. In one embodiment, the fabrication of a disk electrode involves sealing a sharpened internal signal transduction element (“ISTE”) into a substrate, followed by polishing of the substrate until a nanometer-sized disk of the ISTE is exposed. The fabrication of a nanopore electrode is accomplished by etching the nanodisk electrode to create a pore in the substrate, with the remaining ISTE comprising the pore base. Complete removal of the ISTE yields a nanopore membrane, in which a conical shaped pore is embedded in a thin membrane of the substrate. | 07-14-2011 |
20110171518 | Three dimensional Battery Architectures and Methods of Making Same - A three-dimensional electrode structure for use in a battery comprising a porous three-dimensional substrate formed from a first electrically conductive material, an ion-conducting dielectric material disposed on the porous three dimensional substrate, and a second electrically conductive material disposed on the ion-conducting dielectric material, wherein the ion-conducting dielectric material separates the first electrically conductive material from the second electrically conductive material. | 07-14-2011 |
20120091005 | DETECTION OF NUCLEIC ACID LESIONS AND ADDUCTS USING NANOPORES - Methods, systems, and compounds for detecting modified nucleic acid bases are disclosed and described. The methods provide for detecting a nucleic acid lesion and can include directing a nucleic acid adduct into a channel, wherein the nucleic acid adduct includes a nucleic acid having a lesion and a current modulating compound coupled to the nucleic acid at the lesion ( | 04-19-2012 |