Patent application number | Description | Published |
20080274143 | Chloroplasts Engineering to Express Pharmaceutical Proteins - Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants. | 11-06-2008 |
20080311139 | Plant Produced Vaccine for Amebiasis - Disclosed herein are methods of making a vaccine against | 12-18-2008 |
20090007294 | Genetic Engineering of Male Sterility in Plants - Disclosed herein are methods of achieving male sterility in plants. Specifically exemplified herein is the transformation of the plastid genome with a vector expressing the phaA gene. Expression of the phaA gene in plastids results in plants that do not exhibit pleiotropic effects with the exception of male sterility. Also disclosed are stably transformed plants and cells, as well as example vectors for expressing the phaA gene in plastids. | 01-01-2009 |
20090022705 | CHLOROPLASTS ENGINEERED TO EXPRESS PHARMACEUTICAL PROTEINS IN EDIBLE PLANTS - Disclosed herein are compositions for conferring oral tolerance in mammals to autoimmune disorders such as diabetes, as well as vectors and methods for plastid transformation of plants to produce a CTB-Pins protein for oral delivery. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to plants edible without torrefying. Exemplified herein is the stable transformation of | 01-22-2009 |
20090077695 | Interferon Produced In Plastids - Disclosed herein is an expression cassette containing a polynucleotide encoding an IFNα2b polypeptide and having two overlapping primers at a 5′ end encoding a polyhistidine tag and a thrombin cleavage site fused to the polypeptide, the expression cassette carried by a vector competent for integrating the expression cassette in a plastid genome. Also disclosed is a transgenic plastid, preferably a chloroplast, containing a genome transformed by integration of an expression cassette having a non-plant gene encoding an IFNα2b polypeptide and having regions that encode a polyhistidine tag and a thrombin cleavage site fused with the IFNα2b polypeptide. | 03-19-2009 |
20090083885 | PLANT PRODUCED VACCINE FOR AMEBIASIS - Disclosed herein are methods of making a vaccine against Entamoeba histolytica and methods of immunizing a subject using such vaccine. Specifically exemplified are plants expressing a LecA polypeptide and plant material obtained from such plant being used as a basis for vaccination | 03-26-2009 |
20090297550 | Chloroplast-derived human vaccine antigens against malaria - Disclosed is a method of making a malaria vaccine, the method comprising stably transforming a plant by inserting into its plastid genome a nucleic acid sequence encoding and operable to constitutively express a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both; harvesting the stably transformed plant in whole or in part; purifying the expressed malaria antigenic polypeptide from the harvested plant; and packaging the purified antigenic polypeptide under sterile conditions in an amount for a predetermined dosage. Also disclosed is an oral vaccine effective in raising malaria antibodies in a susceptible host, the vaccine comprising leaf material from an edible plant containing plastids stably transformed to constitutively express a fusion polypeptide consisting essentially of cholera toxin B subunit and a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both. | 12-03-2009 |
20090325240 | PRODUCTION AND USE OF PLANT DEGRADING MATERIALS - Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol. | 12-31-2009 |
20100144633 | CHLOROPLAST EXPRESSION OF MEMBRANE PROTEINS - Disclosed herein are chloroplast transformation vectors constructed to enable expression and hyperaccumulation of membrane proteins in chloroplasts. Another embodiment relates to plants transformed with such vectors. Another embodiment relates to seeds and other plant tissues transformed with such vectors. Another embodiment relates to a method of increasing expression of membrane proteins in chloroplasts including transforming a plant cell with vectors described herein. | 06-10-2010 |
20100251425 | Expression of human interferon in transgenic chloroplasts - A plastid transformation vector for a stably transforming a plastid genome is provided. The vector includes, as operably-linked components, a first flanking sequence, a DNA sequence coding for a therapeutic human IFN, which is capable of expression in the plastid and a second flanking sequence. The invention also provides isolated and purified IFN, wherein the IFN is configured in a monomeric or multimeric form and is a structural equivalent to orally administered human IFN. Also provided are methods for variable-expressing biopharmaceutical proteins in plants suitable for mammal consumption. The method includes integrating a plastid transformation vector into a plastid genome of a plant cell; growing the plant cell to express a biopharmaceutical protein, such as therapeutic human interferon IFN. Also disclosed are plants transformed with the aforementioned vectors, and the progeny thereof. Also, disclosed is the IFN, which is IFNα2b. | 09-30-2010 |
20100266640 | Plant-Derived Cholera and Malaria Vaccine - Described herein are methods for simultaneously immunizing a subject against Cholera and Malarial infection. Specifically exemplified herein are methods that involve administering compositions comprising a CTB-AMA1 or CTB-MSP1 derived from plants having plastids transformed to express such conjugates. | 10-21-2010 |
20100278869 | Plant Produced Vaccine for Amebiasis - Disclosed herein are methods of making a vaccine against | 11-04-2010 |
20100304476 | Expression of Human Serum Albumin in Plastids - Human Serum Albumin (HSA) or an HSA fusion protein is expressed in plant plastids. A plastid transformation vector is made which contains an expression cassette that contains regulatory sequences, the coding region for HSA or an HSA fusion protein and a selectable marker coding sequence. The vector is used to transform a plant where the plant expresses the HSA or HSA fusion protein. HSA is isolated and purified from the plant. A preferred plant is tobacco. | 12-02-2010 |
20100325756 | PLASTID TRANSFORMATION UTILIZING ENDOGENOUS REGULATORY ELEMENTS - Disclosed herein are vectors, plants and methods of transforming plants to increase translation efficiency. Specifically disclosed are methods of implementing a regulatory element endogenous to a target plant species and operatively associating said regulatory element with a heterologous gene of interest. Examples of regulatory sequences are disclosed including 5′ UTR sequences of chloroplast genes, such as a psbA gene. | 12-23-2010 |
20110072541 | MARKER FREE TRANSGENIC PLANTS ENGINEERING THE CHLOROPLAST GENOME WITHOUT THE USE OF ANTIBIOTIC SELECTION - The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting an antibiotic-free phototoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation. | 03-24-2011 |
20110110981 | Orally Administerable Vaccine for Yersinia Pestis - Disclosed herein is the successful expression of the plague F1-V fusion antigen in chloroplasts. Parenteral and/or oral administration of chloroplast produced antigens effectively elicit protective immune responses in vivo. Disclosed herein is the first report of a plant-derived oral vaccine that protected animals from live | 05-12-2011 |
20110179530 | Pharmaceutical Proteins, Human Therapeutics, Human Serum Albumin Insulin, Native Cholera Toxin B Subunit on Transgenic Plastids - This invention relates in part to synthesizing high value pharmaceutical proteins in transgenic plants by chloroplast expression for pharmaceutical protein production. We use poly(GVGVP), for example, as a fusion protein to enable hyper-expression of insulin and to accomplish rapid one step purification of fusion peptides utilizing the inverse temperature transition properties of this polymer. We also use insulin-CTB fusion protein in chloroplasts of nicotine free edible tobacco (LAMD 605) for oral delivery. This invention includes expression of native cholera toxin B subunit gene as oligomers in transgenic tobacco chloroplasts which may be utilized in connection with large-scale production of purified CTB, as well as an edible vaccine if expressed in an edible plant, as a transmucosal carrier of peptides to which it is fused to enhance mucosal immunity, and/or to induce oral tolerance of the products of these peptides. The present invention also relates in part to recombinant DNA vectors for enhanced expression of human serum albumin, insulin-like growth factor I, and interferon-α 2 and 5, via chloroplast genomes. | 07-21-2011 |
20110302675 | CONTROL OF VIRAL AND BACTERIAL INFECTION BY ANTIMICROBIAL PEPTIDES RETROCYLIN AND/OR PROTEGRIN EXPRESSED IN CHLOROPLASTS - Disclosed herein are antimicrobial compositions containing one or more antimicrobial peptides having been expressed in chloroplasts. Exemplified herein are the expression and use of retrocylin and protegrin. Disclosed herein are methods of engineering chloroplasts to express such antimicrobial peptides such that they are properly processed and active. Plants containing such chloroplasts are disclosed as well. The chloroplast expressed peptides are useful to delay, prevent or treat viral and bacterial infections. | 12-08-2011 |
20130007926 | ADMINISTRATION OF PLANT EXPRESSED ORAL TOLERANCE AGENTS - Protein replacement therapy for patients with hemophilia or other inherited protein deficiencies is often complicated by pathogenic antibody responses, including antibodies that neutralize the therapeutic protein or that predispose to potentially life-threatening anaphylactic reactions by formation of IgE. Using murine hemophilia B as a model, we have developed a prophylactic protocol against such responses that is non-invasive and does not include immune suppression or genetic manipulation of the patient's cells. Oral delivery of coagulation factor IX (F. IX) expressed in chloroplasts, bioencapsulated in plant cells, effectively blocked formation of inhibitory antibodies in protein replacement therapy. Inhibitor titers were mostly undetectable and up to 100-fold lower in treated mice when compared to controls. Moreover, this treatment eliminated fatal anaphylactic reactions that occurred after 4 to 6 exposures to intravenous F. IX protein. While only 20-25% of control animals survived after 6-8 F. IX doses, 90-95% of tolerized mice survived 12 injections without signs of allergy or anaphylaxis. This high-responder strain of hemophilia B mice represents the first hemophilic animal model to study anaphylactic reactions. The plant material was effective over a range of oral antigen doses (equivalent to 5-80 μg recombinant F.IX/kg), and controlled inhibitor formation and anaphylaxis long-term, up to 7 months. Oral antigen administration caused a deviant immune response that suppressed formation of IgE and inhibitory antibodies. This cost-effective and efficient approach to oral delivery of protein antigens to the gut should be applicable to several genetic diseases that are prone to pathogenic antibody responses during treatment. | 01-03-2013 |
20130058969 | CHLOROPLASTS ENGINEERED TO EXPRESS PHARMACEUTICAL PROTEINS - Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants. | 03-07-2013 |
20130137142 | Expression of Beta-Mannanase in Chloroplasts and its Utilization in Lignocellulosic Woody Biomass Hydrolysis - Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol. In other exemplary embodiments, methods and materials are disclosed for degrading mannans. | 05-30-2013 |
20130149328 | PLANT-DERIVED CHOLERA AND MALARIA VACCINE - Described herein are methods for simultaneously immunizing a subject against Cholera and Malarial infection. Specifically exemplified herein are methods that involve administering compositions comprising a CTB-AMA1 or CTB-MSP1 derived from plants having plastids transformed to express such conjugates. | 06-13-2013 |
20130160166 | ORAL INSULIN THERAPY - This disclosure relates in part to synthesizing a cleavable proinsulin construct in transgenic plants by chloroplast expression. | 06-20-2013 |
20130189308 | EXPRESSION OF PROTECTIVE ANTIGENS IN TRANSGENIC CHLOROPLASTS AND THE PRODUCTION OF IMPROVED VACCINES - Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The invention further provides transformed plastids having the ability to survive selection in both the light and the dark, at different developmental stages by using genes coding for two different enzymes capable of detoxifying the same selectable marker, driven by regulatory signals that are functional in proplastids as well as in mature chloroplasts. The invention utilizes antibiotic-free selectable markers to provide edible vaccines for conferring immunity to a mammal against | 07-25-2013 |
20130202647 | Chloroplast Expression of Membrane Proteins - Disclosed herein are chloroplast transformation vectors constructed to enable expression and hyperaccumulation of membrane proteins in chloroplasts. Another embodiment relates to plants transformed with such vectors. Another embodiment relates to seeds and other plant tissues transformed with such vectors. Another embodiment relates to a method of increasing expression of membrane proteins in chloroplasts including transforming a plant cell with vectors described herein. | 08-08-2013 |
20140033367 | AGRONOMIC TRAITS VIA GENETICALLY INDUCED ELEVATION OF PHYTOHORMONE LEVELS IN PLANTS - Disclosed herein are transgenic plants that have been modified to express β-glucosidase, and methods and materials for making same. The transgenic plants possess several advantageous features including increased biomass, increased trichome density and increased parasite resistance. | 01-30-2014 |
20140101792 | PLASTID TRANFORMATION UTILIZING ENDOGENOUS REGULATORY ELEMENTS - Disclosed herein are vectors, plants and methods of transforming plants to increase translation efficiency. Specifically disclosed are methods of implementing a regulatory element endogenous to a target plant species and operatively associating said regulatory element with a heterologous gene of interest. Examples of regulatory sequences are disclosed including 5′ UTR sequences of chloroplast genes, such as a psbA gene. | 04-10-2014 |
20140127266 | PLANT PRODUCED VACCINE FOR AMEBIASIS - Disclosed herein are methods of making a vaccine against | 05-08-2014 |
20140294895 | Expression of Protective Antigens in Transgenic Chloroplasts and the Production of Improved Vaccines - Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The invention further provides transformed plastids having the ability to survive selection in both the light and the dark, at different developmental stages by using genes coding for two different enzymes capable of detoxifying the same selectable marker, driven by regulatory signals that are functional in proplastids as well as in mature chloroplasts. The invention utilizes antibiotic-free selectable markers to provide edible vaccines for conferring immunity to a mammal against | 10-02-2014 |