| Patent application number | Description | Published |
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| 20080274143 | Chloroplasts Engineering to Express Pharmaceutical Proteins - Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants. | 11-06-2008 |
| 20080311139 | Plant Produced Vaccine for Amebiasis - Disclosed herein are methods of making a vaccine against | 12-18-2008 |
| 20090007294 | Genetic Engineering of Male Sterility in Plants - Disclosed herein are methods of achieving male sterility in plants. Specifically exemplified herein is the transformation of the plastid genome with a vector expressing the phaA gene. Expression of the phaA gene in plastids results in plants that do not exhibit pleiotropic effects with the exception of male sterility. Also disclosed are stably transformed plants and cells, as well as example vectors for expressing the phaA gene in plastids. | 01-01-2009 |
| 20090022705 | CHLOROPLASTS ENGINEERED TO EXPRESS PHARMACEUTICAL PROTEINS IN EDIBLE PLANTS - Disclosed herein are compositions for conferring oral tolerance in mammals to autoimmune disorders such as diabetes, as well as vectors and methods for plastid transformation of plants to produce a CTB-Pins protein for oral delivery. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to plants edible without torrefying. Exemplified herein is the stable transformation of | 01-22-2009 |
| 20090077695 | Interferon Produced In Plastids - Disclosed herein is an expression cassette containing a polynucleotide encoding an IFNα2b polypeptide and having two overlapping primers at a 5′ end encoding a polyhistidine tag and a thrombin cleavage site fused to the polypeptide, the expression cassette carried by a vector competent for integrating the expression cassette in a plastid genome. Also disclosed is a transgenic plastid, preferably a chloroplast, containing a genome transformed by integration of an expression cassette having a non-plant gene encoding an IFNα2b polypeptide and having regions that encode a polyhistidine tag and a thrombin cleavage site fused with the IFNα2b polypeptide. | 03-19-2009 |
| 20090083885 | PLANT PRODUCED VACCINE FOR AMEBIASIS - Disclosed herein are methods of making a vaccine against Entamoeba histolytica and methods of immunizing a subject using such vaccine. Specifically exemplified are plants expressing a LecA polypeptide and plant material obtained from such plant being used as a basis for vaccination | 03-26-2009 |
| 20090297550 | Chloroplast-derived human vaccine antigens against malaria - Disclosed is a method of making a malaria vaccine, the method comprising stably transforming a plant by inserting into its plastid genome a nucleic acid sequence encoding and operable to constitutively express a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both; harvesting the stably transformed plant in whole or in part; purifying the expressed malaria antigenic polypeptide from the harvested plant; and packaging the purified antigenic polypeptide under sterile conditions in an amount for a predetermined dosage. Also disclosed is an oral vaccine effective in raising malaria antibodies in a susceptible host, the vaccine comprising leaf material from an edible plant containing plastids stably transformed to constitutively express a fusion polypeptide consisting essentially of cholera toxin B subunit and a malaria antigenic polypeptide selected from AMA-1, MSP-1 or both. | 12-03-2009 |
| 20090325240 | PRODUCTION AND USE OF PLANT DEGRADING MATERIALS - Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol. | 12-31-2009 |
| 20100144633 | CHLOROPLAST EXPRESSION OF MEMBRANE PROTEINS - Disclosed herein are chloroplast transformation vectors constructed to enable expression and hyperaccumulation of membrane proteins in chloroplasts. Another embodiment relates to plants transformed with such vectors. Another embodiment relates to seeds and other plant tissues transformed with such vectors. Another embodiment relates to a method of increasing expression of membrane proteins in chloroplasts including transforming a plant cell with vectors described herein. | 06-10-2010 |
| 20100251425 | Expression of human interferon in transgenic chloroplasts - A plastid transformation vector for a stably transforming a plastid genome is provided. The vector includes, as operably-linked components, a first flanking sequence, a DNA sequence coding for a therapeutic human IFN, which is capable of expression in the plastid and a second flanking sequence. The invention also provides isolated and purified IFN, wherein the IFN is configured in a monomeric or multimeric form and is a structural equivalent to orally administered human IFN. Also provided are methods for variable-expressing biopharmaceutical proteins in plants suitable for mammal consumption. The method includes integrating a plastid transformation vector into a plastid genome of a plant cell; growing the plant cell to express a biopharmaceutical protein, such as therapeutic human interferon IFN. Also disclosed are plants transformed with the aforementioned vectors, and the progeny thereof. Also, disclosed is the IFN, which is IFNα2b. | 09-30-2010 |
| 20100266640 | Plant-Derived Cholera and Malaria Vaccine - Described herein are methods for simultaneously immunizing a subject against Cholera and Malarial infection. Specifically exemplified herein are methods that involve administering compositions comprising a CTB-AMA1 or CTB-MSP1 derived from plants having plastids transformed to express such conjugates. | 10-21-2010 |
| 20100278869 | Plant Produced Vaccine for Amebiasis - Disclosed herein are methods of making a vaccine against | 11-04-2010 |
| 20100304476 | Expression of Human Serum Albumin in Plastids - Human Serum Albumin (HSA) or an HSA fusion protein is expressed in plant plastids. A plastid transformation vector is made which contains an expression cassette that contains regulatory sequences, the coding region for HSA or an HSA fusion protein and a selectable marker coding sequence. The vector is used to transform a plant where the plant expresses the HSA or HSA fusion protein. HSA is isolated and purified from the plant. A preferred plant is tobacco. | 12-02-2010 |
| 20100325756 | PLASTID TRANSFORMATION UTILIZING ENDOGENOUS REGULATORY ELEMENTS - Disclosed herein are vectors, plants and methods of transforming plants to increase translation efficiency. Specifically disclosed are methods of implementing a regulatory element endogenous to a target plant species and operatively associating said regulatory element with a heterologous gene of interest. Examples of regulatory sequences are disclosed including 5′ UTR sequences of chloroplast genes, such as a psbA gene. | 12-23-2010 |
| 20110072541 | MARKER FREE TRANSGENIC PLANTS ENGINEERING THE CHLOROPLAST GENOME WITHOUT THE USE OF ANTIBIOTIC SELECTION - The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting an antibiotic-free phototoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation. | 03-24-2011 |
| 20110110981 | Orally Administerable Vaccine for Yersinia Pestis - Disclosed herein is the successful expression of the plague F1-V fusion antigen in chloroplasts. Parenteral and/or oral administration of chloroplast produced antigens effectively elicit protective immune responses in vivo. Disclosed herein is the first report of a plant-derived oral vaccine that protected animals from live | 05-12-2011 |
