Patent application number | Description | Published |
20100330572 | ORGANIC COMPOUNDS - The present invention relates to a novel selection system for use in a eukaryotic cell culture process and for expression of a recombinant product of interest. The selection system is based on the introduction of an exogenous functional membrane-bound folate receptor gene together with the polynucleotide or gene encoding the product of interest into a eukaryotic cell and can be widely utilized with eukaryotic cells for which cellular viability is dependent upon folic acid uptake. | 12-30-2010 |
20110045536 | MAMMALIAN EXPRESSION VECTOR - The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising (a) at least one expression cassette (POI) for expressing a polypeptide of interest; (b) an expression cassette (MSM) comprising a mammalian selectable marker gene; (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene; wherein the expression cassette (POI) is flanked 5′ by the expression cassette (MASM), the expression cassette (MSM) is located 3′ from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5′ to 3′ orientation. Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells. | 02-24-2011 |
20110281751 | CELL SURFACE DISPLAY OF POLYPEPTIDE ISOFORMS BY STOP CODON READTHROUGH - The present invention inter alia pertains to a method for generating or selecting a eukaryotic host cell expressing a desired level of a polypeptide of interest, comprising:
| 11-17-2011 |
20110306092 | EXPRESSION VECTOR SYSTEM COMPRISING TWO SELECTION MARKERS - The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least
| 12-15-2011 |
20110306095 | METHODS FOR SELECTING EUKARYOTIC CELLS EXPRESSING A HETEROLOGOUS PROTEIN - The invention pertains to a method for selecting at least one eukaryotic host cell expressing a product of interest, comprising
| 12-15-2011 |
20120271040 | MAMMALIAN EXPRESSION VECTOR - The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising
| 10-25-2012 |
20120276579 | ORGANIC COMPOUNDS - The present invention relates to a novel selection system for use in a eukaryotic cell culture process and for expression of a recombinant product of interest. The selection system is based on the introduction of an exogenous functional membrane-bound folate receptor gene together with the polynucleotide or gene encoding the product of interest into a eukaryotic cell and can be widely utilized with eukaryotic cells for which cellular viability is dependent upon folic acid uptake. | 11-01-2012 |
20140154739 | METHODS FOR SELECTING EUKARYOTIC CELLS EXPRESSING A HETEROLOGOUS PROTEIN - The invention pertains to a method for selecting at least one eukaryotic host cell expressing a product of interest, comprising
| 06-05-2014 |
20140295489 | ORGANIC COMPOUNDS - The present invention relates to a novel selection system for use in a eukaryotic cell culture process and for expression of a recombinant product of interest. The selection system is based on the introduction of an exogenous functional membrane-bound folate receptor gene together with the polynucleotide or gene encoding the product of interest into a eukaryotic cell and can be widely utilized with eukaryotic cells for which cellular viability is dependent upon folic acid uptake. | 10-02-2014 |