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Gibson, MD
Daniel G. Gibson, Crofton, MD US
| Patent application number | Description | Published |
|---|---|---|
| 20090275086 | ASSEMBLY OF LARGE NUCLEIC ACIDS - A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described. | 11-05-2009 |
| 20100035768 | METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES - The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization. | 02-11-2010 |
| 20110053272 | METHODS FOR CLONING AND MANIPULATING GENOMES - Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells. | 03-03-2011 |
| 20110053273 | METHODS FOR CLONING AND MANIPULATING GENOMES - Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells. | 03-03-2011 |
Daniel Glenn Gibson, Crofton, MD US
| Patent application number | Description | Published |
|---|---|---|
| 20100184187 | IN VITRO RECOMBINATION METHOD - The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5′ exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. | 07-22-2010 |
| 20100311126 | METHOD FOR IN VITRO RECOMBINATION - The present invention relates, e.g., to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising (a) chewing back the DNA molecules with an enzyme having an exonuclease activity, to yield single-stranded overhanging portions of each DNA molecule which contain a sufficient length of the region of sequence identity to hybridize specifically to each other; (b) specifically annealing the single-stranded overhangs; and (c) repairing single-stranded gaps in the annealed DNA molecules and sealing the nicks thus formed (ligating the nicked DNA molecules). The region of sequence identity generally comprises at least 20 non-palindromic nucleotides (nt), e.g., at least about 40 non-palindromic nt. In some embodiments of the invention, about 5% PEG is present during all steps of the reaction, and/or the repair reaction is achieved with Taq DNA polymerase and a compatible ligase, such as Taq DNA ligase. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. | 12-09-2010 |
Daniel J. Gibson, Cheverly, MD US
| Patent application number | Description | Published |
|---|---|---|
| 20110002585 | FIBER-BASED MID-IR SIGNAL COMBINER AND METHOD OF MAKING SAME - The present invention is generally directed to a device comprising multiple specialty glass optical fibers that combines several different mid-infrared optical signals from multiple optical fibers into one signal in a single optical fiber. In addition, the present invention provides for a method of making the device. | 01-06-2011 |
Daniel J. Gibson, Greenbelt, MD US
| Patent application number | Description | Published |
|---|---|---|
| 20100303429 | Microstructured Optical Fiber Draw Method with In-Situ Vacuum Assisted Preform Consolidation - A method and apparatus for making a substantially void-free microstructured optical fiber using a one-step process is provided. A preform for the optical fiber is prepared, comprising an outer jacket made of solid glass, a cladding having a plurality of microtubes and/or microcanes arranged in a desired pattern within the jacket, and a core which may be solid or hollow, with the cladding and the core extending above the top of the outer jacket. The thus-prepared preform is placed into a fiber draw tower. As the fiber is drawn, negative gas pressure is applied to draw the canes together and consolidate the interfacial voids between the canes while positive gas pressure is applied to the preform to keep the holes of the microcanes open during the fiber drawing. The apparatus includes a jig having support tubes that are connected to a vacuum pump for application of the negative gas pressure and a vent tube connected to a gas supply for application of the positive gas pressure. The interfaces between the support tube and the outer jacket and between the vent tube and the cladding are sealed to ensure that the appropriate application of negative or positive pressure during the draw step is obtained. The preforms according to the present invention can include one or more components fabricated from specialty non-silica glass. | 12-02-2010 |
| 20110038587 | MULTI-CLAD OPTICAL FIBER - A chalcogenide multi-clad optical fiber having a core, a first cladding and one or more subsequent claddings including a chalcogenide glass. The optical fiber may be capable of transmitting visible and inferred light and may be used for a wide variety of semiconductor applications. | 02-17-2011 |
Daniel Joseph Gibson, Cheverly, MD US
| Patent application number | Description | Published |
|---|---|---|
| 20110013268 | SYSTEMS AND METHODS OF ACHIEVING HIGH BRIGHTNESS INFRARED FIBER PARAMETRIC AMPLIFIERS ADN LIGHT SOURCES - Fiber optic amplification in a spectrum of infrared electromagnetic radiation is achieved by creating a chalcogenide photonic crystal fiber (PCF) structure having a radially varying pitch. A chalcogenide PCF system can be tuned during fabrication of the chalcogenide PCF structure, by controlling, the size of the core, the size of the cladding, and the hole size to pitch ratio of the chalcogenide PCF structure and tuned during exercising of the chalcogenide PCF system with pump laser and signal waves, by changing the wavelength of either the pump laser wave or the signal wave, maximization of nonlinear conversion of the chalcogenide PCF, efficient parametric conversion with low peak power pulses of continuous wave laser sources, and minimization of power penalties and minimization of the need for amplification and regeneration of pulse transmissions over the length of the fiber, based on a dispersion factor. | 01-20-2011 |
Matthew Gibson, Baltimore, MD US
| Patent application number | Description | Published |
|---|---|---|
| 20100227836 | PARENTERAL ADMINISTRATION OF A GLUCOSAMINE - Materials and methods for local delivery of a glucosamine are provided to facilitate bone and cartilage growth. | 09-09-2010 |
Michael Gibson, Baltimore, MD US
| Patent application number | Description | Published |
|---|---|---|
| 20090311226 | COMPOSITION AND METHOD FOR TREATING ESOPHAGEAL DYSPLASIA - Enhancing the immune response to esophageal tumor cells reduces the incidence of esophageal cancer developing, recurring, or metastasizing. Esophageal cancer cells are modified to render them more immunogenic and proliferation compromised. They are used in an antigenic preparation to raise a T cell response in the recipient. | 12-17-2009 |