Patent application number | Description | Published |
20080261237 | Inositol-Phosphate Derivatives and Method of Detecting Inositol-1-Phosphate - The present invention relates to inositol phosphate derivatives, in which the inositol phosphate is substituted with one or two reactive groups G or one or two conjugated substances or molecules M, said reactive group(s) G or said substance(s) or molecule(s) M being linked to IP1 via a linkage group L, M being chosen from the following group: a tracer, an immunogen, a member of a binding partner pair, a solid support. | 10-23-2008 |
20090162861 | METHOD FOR SUPPRESSING A FRET SIGNAL, FRET SIGNAL SUPPRESSOR AGENTS AND USE IN A METHOD FOR MULTIPLEXING BIOLOGICAL EVENTS - The invention relates to a method for suppressing the FRET emitted by a reaction medium containing a pair of fluorescent FRET partner conjugates specific for a biological event, characterized in that a FRET signal killer which does not disturb said biological event is introduced into this medium. | 06-25-2009 |
20090215106 | METHOD FOR DETECTING INTRACELLULAR INTERACTION BETWEEN BIOMOLECULES - The present invention relates to a quantitative non-microscopic method of detecting intracellular interactions between biomolecules, on living cells, in response to a biological or pharmacological stimulation, by a time-resolved proximate energy transfer effect between two members of a fluorescence donor/acceptor pair. | 08-27-2009 |
20090220988 | METHOD OF REVEALING A BIOLOGICAL PROCESS USING A FRET MEASUREMENT - The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps:
| 09-03-2009 |
20090294691 | Method for Improving the Detection of Fluorescence Signals During a Resonance Energy Transfer - The invention concerns the use of the fluorescence polarization phenomenon to improve detection of fluorescent signals during a fluorescence resonance energy transfer (FRET). In particular, the invention concerns a method for improving signal/noise ratio in a FRET measurement. The invention also concerns an apparatus for measuring fluorescence following an energy transfer between a donor fluorescent compound and an acceptor fluorescence compound in a measurement medium. | 12-03-2009 |
20100086943 | METHOD FOR THE DETECTION OF POST-TRANSLATIONAL MODIFICATIONS - Method for the detection in homogeneous medium of a post-translational modification of a protein substrate catalyzed by a cell enzyme, characterized in that the post-translational modification reaction takes place in intact living cells, in that these cells comprise a heterologous expression vector coding for a fusion protein comprising the protein substrate and a first coupling domain and in that it comprises the following stages: (v) Incubation of the cells in the presence or in the absence of a compound to be tested capable of modulating the activity of said enzyme, (vi) Addition to the reaction medium of a first fluorescent compound member of a first pair of FRET partners covalently bonded to a coupling agent capable of binding specifically to the first coupling domain present on the protein substrate, (vii) Addition to the reaction medium of a second fluorescent compound member of this first pair of FRET partners, and covalently bonded to a binding domain specific to the site of the protein substrate having undergone the post-translational modification and not binding to the non-modified protein substrate, (i) Measurement of the FRET signal emitted by the sample, this signal being representative of the quantity of protein substrate having undergone said post-translational modification; and cells for the implementation of said method. | 04-08-2010 |
20140242611 | METHOD FOR DETECTING AND/OR QUANTIFYING AN ANALYTE AT THE SURFACE OF A CELL - The invention relates to a method for quantifying a protein of interest expressed at the surface of a cell or else present in a tissue sample, said method comprising the use of two ligands capable of binding specifically to a domain of said protein. | 08-28-2014 |
20150185150 | METHOD FOR NORMALIZING THE LUMINESCENCE EMITTED BY A MEASURING MEDIUM - The subject matter of the invention is a method for measuring the luminescence of a long-life fluorescent compound present in a measuring medium, said medium containing a biological sample, characterized in that it comprises the following steps:
| 07-02-2015 |