| Patent application number | Description | Published |
|---|
| 20090012282 | 5-METHYLCYTOSINE DETECTION, COMPOSITIONS AND METHODS THEREFOR - Compositions and methods for detecting 5-methylcytosine in a nucleic acid are disclosed. A 5-methylcytosine discriminator, which is a deoxyribonucleosidetriphosphate comprising a cytosine-pairing moiety such as a guanosine and a moiety which hinders hydrogen bonding between the cytosine-pairing moiety and a 5-methylcytosine is described. The discriminator is able to base pair with a cytosine but not a 5-methylcytosine. A 5-methylcytosine comprised by a target nucleotide can be detected in a reaction using a DNA polymerase and a primer hybridized immediately adjacent to the target nucleotide. In the reaction, pyrophosphate released upon incorporation of a dNTP complementary to a target nucleotide is detected. Lack of incorporation of the discriminator, but incorporation of a dGTP, can indicate that the target nucleotide is a 5-methylcytosine. | 01-08-2009 |
| 20090263797 | REVERSIBLE DI-NUCLEOTIDE TERMINATOR SEQUENCING - The present teachings provide methods, compositions, and kits for synthesizing and sequencing nucleic acids. In some embodiments, reversible di-nucleotide compounds are employed along with cleaving reactions that remove a label and a blocking moiety. Improved sequencing efficiency is achieved by the rapid polymerase-mediated incorporation of reversible di-nucleotide compounds. In some embodiments, the di-nucleotides do not contain conventional nucleotide triphosphates, but rather employ amino acid phosphoramidate nucleotides (AAPNs). | 10-22-2009 |
| 20100047796 | Reversible Terminator Nucleotides And Methods of Use - Disclosed herein a reversible terminator nucleotides and methods of use. | 02-25-2010 |
| 20100087641 | Method and Materials for Quaternary Amine Catalyzed Bisulfite Conversion of Cytosine to Uracil - The invention provides methods and materials for the conversion of cytosine to uracil. A nucleic acid, such a gDNA, is reacted with bisulfate, such as magnesium bisulfite, in the presence of a quaternary amine catalyst. Examples of suitable quaternary amine catalysts include but are not limited to quaternary ammonium compounds, quaternary alkyl ammonium salts, quaternary alkyl ammonium halides, quaternary methyl ammonium bromide, quaternary ammonium chloride, tetraethyl ammonium hydroxide, tetraethylammonium chloride, tetrabutyl ammonium chloride, tetrabutyl ammonium bromide. The invention also contemplates kits of premeasured ingredients for carrying out the methods of the invention either on an individual sample or on a plurality of samples. | 04-08-2010 |
| 20100120157 | Methods of Using Sulfur Nucleophiles as Improved Alternatives to Sodium Bisulfite for Methylated DNA Analysis - The invention provides for the use of sulfur nucleophiles in analyzing methylated DNA and novel sulfur nucleophiles suitable for such us. | 05-13-2010 |
| 20100209916 | Apparatus, System, And Method Using Immiscible-Fluid-Discrete-Volumes - Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure. | 08-19-2010 |
| 20110008788 | CHEMICALLY MODIFIED LIGASE COFACTORS, DONORS AND ACCEPTORS - Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. | 01-13-2011 |
| 20110046359 | REVERSIBLE TERMINATOR NUCLEOTIDES AND METHODS OF USE - Disclosed herein a reversible terminator nucleotides and methods of use. | 02-24-2011 |
