Patent application number | Description | Published |
20080213915 | SYSTEM AND METHOD FOR THE MEASUREMENT OF MULTIPLE FLUORESCENCE EMISSIONS IN A FLOW CYTOMETRY SYSTEM - A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed. | 09-04-2008 |
20090176271 | Systems for Efficient Staining and Sorting of Populations of Cells - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 07-09-2009 |
20100248362 | Apparatus and Methods for Sorting Particles - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 09-30-2010 |
20110020855 | Method and apparatus for performing cytometry - Embodiments of the present disclosure generally include a method and apparatus for performing cytometry. Specifically, embodiments of the invention comprise apparatus for providing a sample fluid to a cytometry system comprising a cytometry chip and a holder. The cytometry chip is for channeling a sample fluid from a sample fluid port to an output channel. The holder is configured to retain the cytometry chip within the holder. The holder comprises an interface between the cytometry chip and at least one of a source of the sample fluid, a source of a sheath fluid or a source of electric charge. Embodiments of a method comprise using the apparatus to provide a sample fluid to a cytometry system. | 01-27-2011 |
20110217722 | PHOTO-DAMAGE APPARATUS AND METHODS FOR SORTING PARTICLES - A system and method for sorting a mixture of stained particles in a fluid flow path, including stained particles from two distinguishable groups. The system and method can include an electromagnetic radiation source for exciting fluorescence emissions from the stained particles, a photodetector for detecting the fluorescence emissions from the stained particles, a processor for classifying the stained particles; and a photo-damaging laser for damaging selected particles in the flow path. | 09-08-2011 |
20110217723 | EPI-ILLUMINATION APPARATUS AND METHODS FOR SORTING PARTICLES - A method and apparatus for sorting stained particles in a fluid stream, each of the method and apparatus including an epi-illumination optics system with a focusing lens. The optics system being operable to direct a beam of electromagnetic radiation through the focusing lens in a forward direction along a beam axis intersecting particles in the fluid stream at said first location so that said particles pass through the beam, resulting in the fluorescence emissions from the particles being directed along said beam axis in a rearward direction. | 09-08-2011 |
20110256575 | DIGITAL SAMPLING APPARATUS AND METHODS FOR SORTING PARTICLES - A system and method for sorting a mixture of stained particles including a digital signal processor for analyzing and classifying the digital information generated from the particles and providing a sorting signal to a sorting system as a function of the analyzed and classified digital information. | 10-20-2011 |
20110269175 | METHODS FOR SORTING PARTICLES - A method of sorting stained particles in a flow cytometer by generating a mathematical model based upon extracted features of waveform pulses produced in response to the stained particles. Waveform pulses can then be analyzed to determine the probability the stained particles belong in either a first or a second population and a decision boundary can be established. Sorting decisions can be made based upon the extracted features of the waveform pulses relative to the decision boundary in combination with other desirability factors for particles or droplets containing the particles. | 11-03-2011 |
20120091366 | SYSTEM AND METHOD FOR THE MEASUREMENT OF MULTIPLE FLUORESCENCE EMISSIONS IN A FLOW CYTOMETRY SYSTEM - A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed. | 04-19-2012 |
20120244610 | PHOTO-DAMAGE APPARATUS FOR SORTING PARTICLES - A system and apparatus for sorting a mixture of stained particles in a fluid flow path, including stained particles. The system can include a pulsed electromagnetic radiation source for exciting fluorescence emissions from the stained particles, a photodetector for detecting the fluorescence emissions from the stained particles, a processor for classifying the stained particles; and a photo-damaging laser for damaging selected particles in the flow path. | 09-27-2012 |
20130149736 | METHODS FOR SORTING PARTICLES - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 06-13-2013 |
20130171683 | FLOW CYTOMETER APPARATUS AND METHOD - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 07-04-2013 |
20130224726 | METHOD AND APPARATUS FOR CALIBRATING A FLOW CYTOMETER - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 08-29-2013 |
20130224727 | SYSTEMS AND METHODS FOR SORTING PARTICLES - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 08-29-2013 |
20130224733 | METHOD AND APPARATUS FOR SORTING PARTICLES - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 08-29-2013 |
20130224786 | FLOW CYTOMETER APPARATUS AND METHOD - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 08-29-2013 |
20130224787 | FLOW CYTOMETER METHOD AND APPARATUS - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 08-29-2013 |
20140212917 | COMPENSATION OF MOTION-RELATED ERROR IN A STREAM OF MOVING MICRO-ENTITIES - Apparatus and methods for detecting, characterizing, and compensating motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity within the stream. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter of the system. The pulse jitter can be compensated, according to one embodiment, by adjusting data acquisition observation windows. For the flow cytometer, a reduction of pulse jitter can improve measurement accuracy, resolution of doublets, system throughput, and enable an increase in an interrogation region for probing the micro-entities. | 07-31-2014 |
20140220620 | MULTI-CHANNEL SYSTEM AND METHODS FOR SORTING PARTICLES - A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams. | 08-07-2014 |
20140220621 | CHARACTERIZATION OF MOTION-RELATED ERROR IN A STREAM OF MOVING MICRO-ENTITIES - Apparatus and methods for detecting and characterizing motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter related to a system clock. The motion-related error may be characterized by correlating measurements of micro-entities moving within a fluid stream. | 08-07-2014 |