| 20100089757 | GRADIENT ELUTION ELECTROPHORESIS AND DETECTORLESS ELECTROPHORESIS APPARATUS - A microfluidic apparatus and method for performing electrophoretic separation of compounds. The apparatus comprises: a) A first container for containing a sample fluid; b) a second container for containing a separation buffer fluid; c) a channel of a first length having an inlet end and an outlet end, the inlet end connected to the first container and the outlet end connected to the second container; d) a voltage device electrically connected to the first container and the second container, the voltage device facilitating adjustment of the amount of voltage to the first container and the second container; e) a controller for controlling the velocity flow of the sample fluid through the channel from the first container towards the second container; and f) a measuring device for measuring the current through the channel. The method comprises the steps of: a) providing a separation buffer; b) providing a sample solution in fluid contact with the separation buffer; c) applying an electric field to the separation buffer; and d) producing a variable bulk flow of the separation buffer in a direction substantially aligned with said electric field. Fluid contact between the separation buffer and the sample solution is made through a separation column having a length in the range of from approximately 0.01 mm to approximately 5 mm. By the foregoing, compounds can be sequentially detected and quantified. | 04-15-2010 |
