Patent application number | Description | Published |
20100116486 | WELL ASSEMBLY HAVING A CASING HANGER SUPPORTED BY A LOAD MEMBER ACTUATED BY A RETRACTABLE MEMBER DISPOSED IN THE WELLHEAD - A wellhead system comprising a high pressure housing having a retractable ring and a casing hanger having an activation ring configured to be engaged by the retractable ring when the casing hanger is lowered into the high pressure housing. An expandable load member is carried by the casing hanger into the high pressure housing. When the activation ring is engaged by the retractable ring, the activation ring is blocked from further downward motion. The activation ring and the body of the casing hanger cooperate with the load member to expand the load member outward into engagement with the high pressure housing as the casing hanger is lowered further into the high pressure housing. The outer surface of the load member and the inner surface of the high pressure housing are configured so that the high pressure housing supports the casing hanger when the load member is expanded into engagement with the high pressure housing. | 05-13-2010 |
20100243244 | LOCATION-AND-ROTATION FEEDBACK TOOL FOR SUBSEA WELLHEADS AND METHOD OF OPERATING SAME - A location-and-rotation feedback tool that provides accurate feedback of the location and orientation of a well component during running operations in wells. The feedback tool device is adapted to be secured on a tool stem above a running tool and comprises a body, a circumferentially extending groove, and a hole in the groove that vents to the bore of a blowout preventer. The circumferential groove aligns with a fluid line port in the blowout preventer to provide location feedback via a fluid line pressure. The hole aligns with the fluid line port in the blowout preventer to provide rotation feedback via fluid line pressure. | 09-30-2010 |
20100243271 | BIT-RUN NOMINAL SEAT PROTECTOR AND METHOD OF OPERATING SAME - A drilling system employs a set of bushings that protect seats used for casing hangers. The system includes one or more seat protectors that attach to a running tool. The running tool is lowered down the wellbore with the seat protectors attached and deposits a bushing at each surface to be protected. The running tool may retrieve the bushings as the running tool is withdrawn from the wellbore. | 09-30-2010 |
20120186821 | LOCATION-AND-ROTATION FEEDBACK TOOL FOR SUBSEA WELLHEADS AND METHOD OF OPERATING SAME - A location-and-rotation feedback tool that provides accurate feedback of the location and orientation of a well component during running operations in wells. The feedback tool device is adapted to be secured on a tool stem above a running tool and comprises a body, a circumferentially extending groove, and a hole in the groove that vents to the bore of a blowout preventer. The circumferential groove aligns with a fluid line port in the blowout preventer to provide location feedback via a fluid line pressure. The hole aligns with the fluid line port in the blowout preventer to provide rotation feedback via fluid line pressure. | 07-26-2012 |
20120241162 | CASING HANGER LOCKDOWN SLIP RING - A seal seals an annulus in a subsea assembly between a wellhead and a casing hanger landed on a shoulder within a bore of the wellhead. The seal includes a casing hanger seal ring disposed within the annulus. The seal ring engaged with an inner diameter surface of the wellhead, and engaged with an outer diameter surface of the casing hanger so that the seal ring prevents flow through the annulus. A lockdown slip ring is secured to a lower end of the seal ring so that, when the seal ring is energized, the lockdown slip ring engages a substantially smooth inner diameter surface portion of the wellhead and a substantially smooth outer diameter surface portion of the casing hanger to limit upwards axial movement of the casing hanger. | 09-27-2012 |
20120247788 | SEAL WITH BELLOWS STYLE NOSE RING - A seal assembly between a wellhead housing having a bore and a casing hanger, has an inner seal leg for sealing against hanger and an outer seal leg for sealing against housing. An extension extends downward from outer seal leg and has a downward facing shoulder that rests on an upward facing shoulder formed on a nose ring. Connection connects seal ring to the nose ring with a lower portion of the nose ring resting on the upward facing shoulder of the casing hanger. Bellows are formed on the nose ring to increase lockdown capacity. Bellows have an inner surface that faces an outer profile of the hanger, and an outer surface on the bellow that faces the bore of the housing. When the bellows are axially collapsed, they expand radially outward and contract radially inward into the bore of the housing and the outer profile of the hanger. | 10-04-2012 |
20130140042 | SEAL WITH BELLOWS STYLE NOSE RING AND RADIALLY DRIVABLE LOCK RINGS - A seal assembly between a wellhead housing having a bore and a casing hanger, has an inner seal leg for sealing against a hanger and an outer seal leg for sealing against the housing. A bellows style portion is formed on a nose ring carried by the seal assembly to increase lockdown capacity. The bellows style portion has an inner surface that faces an outer profile of the hanger, and an outer surface that faces the bore of the housing. Inner and outer lock rings are disposed on the bellows style portion. When the bellows style portion is axially collapsed, it radially expands into the bore of the housing and the outer profile of the hanger, and urges the inner and outer lock rings into engagement with the outer profile of the hanger and the bore of the housing. | 06-06-2013 |
20130146306 | CASING HANGER LOCKDOWN WITH CONICAL LOCKDOWN RING - A seal seals an annulus in a subsea assembly between a wellhead and a casing hanger landed on a shoulder within a bore of the wellhead. The seal includes a casing hanger seal ring disposed within the annulus. The seal ring engaged with an inner diameter surface of the wellhead, and engaged with an outer diameter surface of the casing hanger so that the seal ring prevents flow through the annulus. A nose ring is secured to a lower end of the seal ring so that, when the seal ring is energized, a conical surface of the nose ring engages a mating conical profile formed in the inner diameter surface portion of the wellhead and the nose ring engages a surface opposite the conical surface with a substantially smooth outer diameter surface portion of the casing hanger to limit upwards axial movement of the casing hanger. | 06-13-2013 |
20130248171 | SUBSEA MULTIPLE ANNULUS SENSOR - A wellbore assembly includes a housing member, an outer wellbore member, and a second wellbore member, with an outer sensor located in the annulus between the outer wellbore member and the second wellbore member. The outer sensor can sense a condition of the annulus, such as pressure or temperature, and transmit data through a solid portion of the sidewall of the outer wellbore member to a signal receiver located on the housing member. In one embodiment, the signal receiver can transmit an electromagnetic field to inductively charge a power supply on the outer sensor. | 09-26-2013 |
20140167408 | Ratcheting Anti-Rotation Lock for Threaded Connectors - A pipe connection includes a pin having external threads and a box having internal threads. A circumferentially extending row of pin teeth are located on an exterior portion of the pin. A slot extends through a side wall of the box. A key is carried in the slot, the key having a row of key teeth that mate with the box teeth. The key is mounted to the box such that a movable portion of the key is radially movable relative to the box between radially outward and radially inward positions. The key is biased toward the inward position, so that the key teeth ratchet on the pin teeth during make-up of pin and the box. The teeth have a saw-tooth configuration to resist unscrewing rotation of pin and the box. The key may be an integral part of the side wall of the box or a separate component. | 06-19-2014 |
Patent application number | Description | Published |
20130140775 | Seal With Bellows Type Nose Ring - A wellhead assembly having concentric tubulars and a seal assembly in an annulus between the tubulars. The seal assembly includes an upper portion having an annular body with a U-shaped cross section and a nose ring seal. The nose ring seal has a bellows like cross section and provides sealing in the annulus and exerts a lockdown force onto one of the tubulars. Metal inlays are provided on the outer edges of the folds in the bellows and a compressible medium is disposed between the folds. | 06-06-2013 |
20140069631 | Pin-Actuated Lock Ring Arrangement - In a wellhead near the top of an oil and gas well, a locking ring assembly for locking an inner tubular wellhead member axially relative to an outer tubular wellhead member. The inner tubular member has a circumferential recess that contains a locking ring. After insertion of the inner tubular member into the outer tubular member, actuating pins radially expand the locking ring so that it partially leaves the circumferential recess and engages a corresponding recess in the outer tubular member. The pins may be deployed by a seal inserted above the inner tubular member. | 03-13-2014 |
20140069632 | Load Enhanced Locking Arrangement - In a wellhead near the top of an oil and gas well, a locking profile configured for locking engagement with an inner well member and including a locking ridge having a rib and a locking shoulder. The locking ridge extends radially inward from an inner surface of the wellhead into a well bore. The rib is located on an end of the ridge distal from the wellhead and protrudes into the inner well member. The locking shoulder is located on an end of the ridge distal from the wellhead and adjacent a lower end of the rib, so that as the inner well member exerts an upward force on the rib, the upward force creates a moment in the locking ridge that pushes the locking shoulder into tighter engagement with the inner well member. | 03-13-2014 |
20140096977 | SEMI-RIGID LOCKDOWN DEVICE - A wellhead seal assembly that forms a metal-to-metal seal between inner and outer wellhead members. A seal member has inner and outer seal legs separated by a slot. A locking ring is positioned in the outer seal leg and has wickered sections, where each of the wickered sections protrude through a window in the outer seal leg of the seal member. An energizing ring has an upper tapered surface that is oblique to an axis of the annular energizing ring and extending laterally from the axis over a portion of the locking ring and a lower tapered surface that is oblique to and extending laterally from the axis. As the energizing ring is moved into the slot, the upper tapered surface engages an inner surface of the locking ring, and the lower tapered surface engages an inner annular wall of the slot. | 04-10-2014 |
20140103645 | ANTI-ROTATION SYSTEM FOR BOX AND PIN CONNECTION - An anti-rotation system for use in retaining a threaded connection between a pin and a box. The anti-rotation system includes a key that sets in a recess formed in one of the box or pin. The key is selectively in contact with one of the other of the box or pin, and is activated when the threaded connection begins to decouple. The key is profiled and operates in a cam like fashion to wedge itself between the box and pin when these members begin to decouple and prevents further relative rotation. | 04-17-2014 |
20140125053 | BROACH STYLE ANTI ROTATION DEVICE FOR CONNECTORS - A broach style anti-rotation device for connected tubular members is described. In embodiments, the broach tool is inserted into a slot in an outer diameter surface of the pin, and teeth of the broach, which have a successively greater height when moving from the front to the tail of the broach, cut a slot in a surface of the box. Sidewalls of the broach and its teeth engage shoulders of each slot to prevent the rotation of the tubular members relative to each other. | 05-08-2014 |
20140131054 | SLOTTED METAL SEAL - A seal for sealing an annulus between an outer tubular wellhead member and an inner tubular wellhead member is described. In embodiments, the seal is an annular sealing ring that has a plurality of circumferentially spaced apart sealing ring grooves extending at least from a first end to a second end of the sealing surface of at least one of the wellhead members. When the seal is energized, fouling on the sealing surface is urged toward and then through the slot, axially away from the sealing surface. | 05-15-2014 |
20140183824 | SEAL WITH FLEXIBLE NOSE FOR USE WITH A LOCK-DOWN RING ON A HANGER IN A WELLBORE - A seal system selectively set between coaxial downhole tubulars seals between the tubulars; the system also locks the tubulars together to resist relative axial movement from thermal expansion. The seal system includes a seal element with a nose ring that couples a lock-down ring to both the inner and outer tubulars. Before inserting the seal system between the tubulars, the lock-down ring is disposed in a groove on the inner tubular. Setting the seal system drives a lower tip of the nose ring between the lock-down ring and inner tubular, thereby urging the lock-down ring radially outward. A portion of the lock-down ring remains in the groove, while an outer radial portion of the lock-down ring inserts into a profile on the outer tubular. Axial movement of a tubular transfers force to the other tubular through the lock-down ring, while a minimal amount of force transfers through the seal system. | 07-03-2014 |
20140238699 | WELLHEAD ANNULUS SEAL HAVING A WICKERED SURFACE - An annular seal for use in a wellhead assembly has inner and outer legs that each extend in a direction that is generally parallel with an axis of the seal to define an annular space therebetween. Wickers are provided on an outer surface of the seal, so that when the seal is energized and the legs are urged radially apart from one another, the wickers engage with a mating surface of a downhole tubular. Embedding the wickers into the tubular creates a flow barrier across the interface between the seal and the tubular. The wickers deform the surface of the tubular, which creates a lock down force that opposes relative axial movement of the tubular. | 08-28-2014 |
20150114667 | High Strength Inlay to Improve Lock-Down Capacity in a Wellhead - A wellhead assembly includes a wellhead housing having a bore and a locking profile including a gallery slot, and an annular notch. An inner wellhead assembly is selectively landed in the bore of the wellhead housing, the inner wellhead assembly having a lock ring with a lock ring profile that engages the locking profile. The engaging surface is a sloped downward facing surface at an axially upper end of the gallery slot. The annular notch has a notch engaging profile with a downward facing notch upper shoulder and an upward facing notch lower shoulder. The locking profile includes an inlay, the inlay being located on the notch upper shoulder and the engaging surface. | 04-30-2015 |
20150114668 | Flow-By Holes with Gallery and Channel Arrangement on Wellhead and Tubular Hanger - A wellhead assembly includes a tubular wellhead housing having a bore and an annular gallery slot. The annular gallery slot is defined by an enlarged inner diameter of the bore. A tubular hanger is selectively landed in the bore of the wellhead housing, defining an annular cavity between the bore and an outer diameter of the tubular hanger. The tubular hanger is supported by the wellhead housing with a hanger support located in the annular cavity. A flow-by passage is in fluid communication with the annular cavity at locations above and below the hanger support. The flow-by passage intersects with the gallery slot and intersects an outer radial surface of the tubular hanger. | 04-30-2015 |
20150114669 | Method and System for Retaining a Lock Ring on a Casing Hanger - A retention system for limiting axial and radial movement of a wellbore lock ring. The retention system includes pins for resisting axial movement of the lock ring and assemblies for limiting radial outward movement of the lock ring. The lock ring circumscribes and couples to a wellbore hanger. The pins project radially from the hanger into the lock ring and into slots, where the slots extend a distance along the inner surface of the lock ring. The assemblies also project radially into the hanger and each have a portion that registers with a channel on a lower end of the lock ring. Lock ring outer radial movement is limited by contact between the portions of the assemblies and inner surfaces of the channels. | 04-30-2015 |
20150267479 | Wear Bushing with Hanger Lockdown - A wear bushing assembly protects and locks down a hanger in a wellhead housing; and which includes a wear bushing body that inserts into a wear bushing sleeve. A lock ring extends into registered recesses on the body and sleeve to couple together the body and sleeve. The wellhead housing has a profiled recess circumscribing its inner surface. A lockdown ring selectively mates with the profiled recess; and when mated is in interfering contact with an upper surface of the wear bushing sleeve, thereby coupling the sleeve to the wellhead housing. The sleeve outer surface is profiled to interfere with upward movement of the hanger, so that force is transferred from the hanger to the housing through the sleeve which locks down the housing. | 09-24-2015 |
20150267495 | Insert for Use with Wellhead Housing Having Flow-by Path - A wellhead assembly having an insert that is disposed between a wellhead housing and casing hanger. Axial slots through the insert define a flow path between the wellhead housing and casing hanger. The insert is made from a higher strength material and supports a load exerted between the casing hanger and wellhead housing. The insert is a ring like member, and the slots can more easily be machined in the insert than in the wellhead housing. | 09-24-2015 |
20150345246 | Wellhead Component with Seal Surface and Method of Forming the Seal - A subset wellhead component has a metal seal surface having voids. The voids are initially filled with an elastomeric substrate having hollow beads. When lowered into the subsea assembly, the beads have sufficient strength to resist collapsing under a hydrostatic pressure of liquid within the subsea wellhead assembly to prevent shrinking of a volume of the substrate. Exerting a setting force on the metal seal surface to form the sealing engagement causes the beads to collapse and reduce the volume of the substrate within the voids. The seal surface may be folds of a bellows-type nose ring of a seal assembly. The seal surface may alternately be wickers formed in the bore of the wellhead housing. | 12-03-2015 |
20160040498 | RATCHETED E-RING RETENTION DEVICE - A wellhead assembly includes as outer tubular wellhead member and an inner tubular wellhead member with a seal pocket between them. A seal ring is located in the seal pocket An annular energizing ring urges the seal ring into sealing engagement with the outer tubular wellhead member and the inner tubular wellhead member. A recess is located on an outer diameter of the annular energizing ring or a radially inner diameter of the seal ring, and a ratcheted retainer is on the other. A ratchet clip with a clip profile is located within the recess. The recess and the ratchet slip extend leas than a full circumferential distance around the outer diameter of the annular energizing ring or the radially inner diameter of the seal ring. A retainer profile on the ratcheted retainer selectively engages with the clip profile of the ratchet clip. | 02-11-2016 |
20160076328 | Seal With Soft Material Inlay - A wellhead assembly includes an inner wellhead member with wickers extending outwardly therefrom and an outer wellhead member with wickers extending inwardly therefrom. A seal assembly having an inner leg and an outer leg is inserted between the wellhead members. An energizing ring forces the legs radially apart from each other. An inlay is located along at least one of an inner diameter of the inner leg and an outer diameter of the outer leg, positioned to sealingly engage the wickers. The inlay is located in a single groove configured so that no part of the seal member body contacts an exterior wall of the inner wellhead member or an interior wall of the outer wellhead member. The inlay has a smooth outer surface flush with the diameter and is made of a material softer than the inner wellhead member, the outer wellhead member, and the seal member body. | 03-17-2016 |
20160090811 | Wellbore Sealing With Hybrid Wicker System - A wellbore system includes a sealing assembly for creating an annular seal between wellbore members. The sealing assembly includes a wicker profile defined a first sealing surface for engaging and embedding into a radially adjacent, second sealing surface. The wicker profile includes a first section having a first hardness and a second section adjacent to the first section and having a second hardness greater than the first hardness. Both the first and second sections of wickers are embedded into the second sealing surface, and can thus provide a high degree of both sealing and lockdown performance. | 03-31-2016 |
Patent application number | Description | Published |
20110108719 | Multi-Channel Source Assembly for Downhole Spectroscopy - A multi-channel source assembly for downhole spectroscopy has individual sources that generate optical signals across a spectral range of wavelengths. A combining assembly optically combines the generated signals into a combined signal and a routing assembly that splits the combined signal into a reference channel and a measurement channel. Control circuitry electrically coupled to the sources modulates each of the sources at unique or independent frequencies during operation. | 05-12-2011 |
20110108720 | Multi-Channel Detector Assembly for Downhole Spectroscopy - A multi-channel detector assembly for downhole spectroscopy has a reference detector unit optically coupled to a reference channel of a source and has a measurement detector unit optically coupled to a measurement channel of the source. The reference and measurement detectors detect spectral signals across a spectral range of wavelengths from the reference and measurement channels. Conversion circuitry converts the detected spectral signals into reference signals and measurement signals, and control circuitry processes the reference and measurements signals based on a form of encoding used by the source. Then, the control circuitry can control the output of spectral signals from the source based on the processed signals or scale the measurement signal to correct for source fluctuations or changes in environmental conditions. | 05-12-2011 |
20110108721 | Filter Wheel Assembly for Downhole Spectroscopy - A downhole fluid analysis tool has a tool housing and a fluid analysis device. The tool housing is deployable downhole and has at least one flow passage for a fluid sample. The fluid analysis device is disposed in the tool housing relative to the flow passage. Inside the device, one or more sources generate a combined input electromagnetic signal across a spectrum of wavelengths, and a routing assembly routes generated signals into the reference and measurement signals. At least one wheel having a plurality of filters is rotated to selectively interpose one or more of the filters in the paths of the reference and measurement signals. | 05-12-2011 |
20120081698 | Refractive Index Sensor for Fluid Analysis - A refractive index sensor having one or more sources, an adaptive optical element or scanner, imaging optics, a sensing optic, and one or more detectors. The scanner impinges a signal from the source into the sensing optic and onto a sensor-sample interface at sequential angles of incidence. The detector response increases dramatically to signals reflected from the interface at corresponding sequential angles of reflection equal to or greater than a critical angle. The refractive index sensor also uses an input lens between the scanner and the sensing optic and uses an output lens between the sensing optic and the detector. A processor controls the sensor and can determine index of refraction of the fluid sample based on the detector response and scan rate. The sensor can be used in several operational environments from a laboratory to a downhole tool, such as a formation tester to determine properties in a borehole environment. | 04-05-2012 |
20120081699 | Downhole Gas Breakout Sensor - A downhole fluid analysis tool has a housing and a flow passage for downhole fluid. A device disposed in the tool housing relative to the flow passage has a one or more sources, one or more sensing optics, one or more detectors, and control circuitry. The source generates an input signal. The sensing optic has a refractive index (RI) higher than crude oil and other expected constituents. A sensing surface of the optic optically coupled to the source interfaces with a downhole fluid. When the variable RI of the downhole fluid reaches a defined relationship to the optic's RI, the input signal interacting with the sensing surface experiences total internal reflection, and the reflected signal from the sensing surface remains in the sensing optic and reflects to a detector. The control circuitry monitors the detector's response and indicates gas break out if the response is above a threshold. | 04-05-2012 |
20120298850 | Quaternary Photodetector for Downhole Optical Sensing - Detector assembly for downhole spectroscopy includes a near-infra-red quaternary photodiode that can operate at high temperatures without cooling it to the standard operation temperature range of the photodiode. High temperature operation of the photodiode right shifts the detector assembly's responsivity curve to include wavelengths of up to 2400-nm. The photodiode has manageable dark current at temperatures even at 200° C., and it can be packaged using high temperature construction. The photodiode is operated in photovoltaic mode at high temperatures but can be operated at photoconductive mode at lower temperatures. At least partial cooling can be provided above a predetermined temperature. | 11-29-2012 |
20130020480 | MULTI-CHANNEL SOURCE ASSEMBLY FOR DOWNHOLE SPECTROSCOPY - A multi-channel source assembly for downhole spectroscopy has individual sources that generate optical signals across a spectral range of wavelengths. A combining assembly optically combines the generated signals into a combined signal and a routing assembly that splits the combined signal into a reference channel and a measurement channel. Control circuitry electrically coupled to the sources modulates each of the sources at unique or independent frequencies during operation. | 01-24-2013 |
20130175438 | Quaternary Photodetector for Downhole Optical Sensing - Detector assembly for downhole spectroscopy includes a near-infra-red quaternary photodiode that can operate at high temperatures without cooling it to the standard operation temperature range of the photodiode. High temperature operation of the photodiode right shifts the detector assembly's responsivity curve to include wavelengths of up to 2400-nm. The photodiode has manageable dark current at temperatures even at 200° C., and it can be packaged using high temperature construction. The photodiode is operated in photovoltaic mode at high temperatures but can be operated at photoconductive mode at lower temperatures. At least partial cooling can be provided above a predetermined temperature. | 07-11-2013 |
20140278113 | Real-Time Determination of Formation Fluid Properties Using Density Analysis - Analysis evaluates formation fluid with a downhole tool disposed in a borehole. A plurality of possible constituents is defined for the formation fluid, and constraints are defined for the possible constituents. The constraints can include boundary constraints and constraints on the system's dynamics. The formation fluid is obtained from the borehole with the downhole tool over a plurality of time intervals, and density of the obtained formation fluid is obtained at the time intervals. To evaluate the fluid composition, a state probability distribution of the possible constituents of the obtained formation fluid at the current time interval is computed recursively from that at the previous time interval and by assimilating the current measured density of the obtained formation fluid in addition to the defined boundary/dynamic constraints. The probabilistic characterization of the state of the possible constituents allows, in turn, the probabilistic inference of formation properties such as contamination level and GOR. | 09-18-2014 |
Patent application number | Description | Published |
20090176723 | Methods and compositions involving miRNA and miRNA inhibitor molecules - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 07-09-2009 |
20090306194 | NOVEL COMPOSITIONS FOR THE IN VIVO DELIVERY OF RNAI AGENTS - This application describes emulsion formulations containing neutral phospholipids for delivering RNAi, anti-miRNA, or aptamer agents in vivo. The application also relates to methods of making the formulations, and uses of the formulations as delivery agents. | 12-10-2009 |
20100221789 | HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES - The present invention provides improved methods of attenuating gene expression through the phenomenon of RNA interference. The invention provides methods of synthesis of double stranded RNAs (dsRNAs) of increased potency for use as small interfering RNA (siRNA). Surprisingly and unexpectedly, siRNAs made by the methods of the invention are significantly more potent than previously available siRNAs. | 09-02-2010 |
20110313025 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 12-22-2011 |
20120065248 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 03-15-2012 |
20130143945 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 06-06-2013 |
20130230920 | METHODS AND COMPOSITIONS RELATING TO POLYPEPTIDES WITH RNASE III DOMAINS THAT MEDIATE RNA INTERFERENCE - The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target. | 09-05-2013 |
20130231266 | METHODS AND COMPOSITIONS REDUCING TARGET GENE EXPRESSION USING COCKTAILS OF siRNAS OR CONSTRUCTS EXPRESSING siRNAS - The present invention concerns methods and compositions involving the production or generation of siRNA mixtures or pools capable of triggering RNA-mediated interference (RNAi) in a cell. Compositions of the invention include kits that include reagents for producing or generating siRNA pools. The present invention further concerns methods using polypeptides with RNase III activity for generating siRNA mixtures or pools that effect RNAi, including the generation of a number of RNA molecules to the same target gene. | 09-05-2013 |
20130303591 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 11-14-2013 |
20140031415 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 01-30-2014 |
20140179762 | METHODS AND COMPOSITIONS REDUCING TARGET GENE EXPRESSION USING COCKTAILS OF siRNAS OR CONSTRUCTS EXPRESSING siRNAS - The present invention concerns methods and compositions involving the production or generation of siRNA mixtures or pools capable of triggering RNA-mediated interference (RNAi) in a cell. Compositions of the invention include kits that include reagents for producing or generating siRNA pools. The present invention further concerns methods using polypeptides with RNase III activity for generating siRNA mixtures or pools that effect RNAi, including the generation of a number of RNA molecules to the same target gene. | 06-26-2014 |
20140295543 | METHODS AND COMPOSITIONS RELATING TO POLYPEPTIDES WITH RNASE III DOMAINS THAT MEDIATE RNA INTERFERENCE - The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target. | 10-02-2014 |
20140314833 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 10-23-2014 |
20140348907 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 11-27-2014 |
20140348908 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 11-27-2014 |
20140350086 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 11-27-2014 |
20150275213 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 10-01-2015 |
20150284719 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 10-08-2015 |
20150284720 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 10-08-2015 |
20150299705 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 10-22-2015 |
20150342981 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 12-03-2015 |
20150344881 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 12-03-2015 |
20150344883 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 12-03-2015 |
20150352046 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 12-10-2015 |
20150361431 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 12-17-2015 |
Patent application number | Description | Published |
20100075423 | METHODS AND COMPOSITIONS RELATING TO POLYPEPTIDES WITH RNASE III DOMAINS THAT MEDIATE RNA INTERFERENCE - The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target. | 03-25-2010 |
20100159591 | METHODS AND COMPOSITIONS CONCERNING siRNA'S AS MEDIATORS OF RNA INTERFERENCE - The present invention concerns an isolated siRNA of from about 5 to about 20 nucleotides that mediates RNA interference. Also disclosed are methods of reducing expression of a target gene in a cell comprising obtaining at least one siRNA of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 basepairs in length; and delivering the siRNA into the cell. The siRNAs can be chemically synthesized RNA or an analog of a naturally occurring RNA. | 06-24-2010 |
20100184039 | METHODS AND COMPOSITIONS RELATING TO LABELED RNA MOLECULES THAT REDUCE GENE EXPRESSION - The present invention concerns methods and compositions involving labeled, double-stranded RNA (dsRNA), including siRNA, capable of triggering RNA-mediated interference (RNAi) in a cell. Compositions of the invention include labeled dsRNA for RNAi, which may be a single strand of RNA that basepairs with itself or two separate RNA strands. In some embodiments, the label is fluorescent. The present invention further concerns methods for preparing such composition and kits for implementing such methods. Other methods of the invention include ways of using labeled dsRNA for RNAi. | 07-22-2010 |
20110151558 | METHODS AND COMPOSITIONS FOR REDUCING TARGET GENE EXPRESSION USING COCKTAILS OF siRNAS OR CONSTRUCTS EXPRESSING siRNAS - The present invention concerns methods and compositions involving the production or generation of siRNA mixtures or pools capable of triggering RNA-mediated interference (RNAi) in a cell. Compositions of the invention include kits that include reagents for producing or generating siRNA pools. The present invention further concerns methods using polypeptides with RNase III activity for generating siRNA mixtures or pools that effect RNAi, including the generation of a number of RNA molecules to the same target gene. | 06-23-2011 |
20110257375 | INCREASING EFFICIENCY OF NUCLEIC ACID DELIVERY IN VIVO USING TARGETING CONJUGATES - Described herein is the use of antibody-based delivery agents to target and deliver nucleic acid agents into specific cell types. Herein, we describe methods used that improve the ability of conjugates that load over 3 siRNA per conjugate and target siRNA to cells expressing the appropriate cell surface antigens. We also contemplate the use of antibody, targeting peptides, small molecules, aptamers and all other factors known in the art that can specifically target tissues. In each case, these targeting moieties can be conjugated using chemical crosslinking agents to carriers enabling directed delivery of nucleic acids. | 10-20-2011 |
20110269137 | RAPID AND EFFICIENT ASSAY TO ASSESS THE SEQUENCE AND SIZE OF 3' ENDS OF POLYNUCLEOTIDES - Described herein is an efficient, highly reproducible approach to assess poly(A) tail length on a mRNA specific basis. The embodiments herein have led to the development of a versatile, easy to use kit for biomedical researchers to address the impact of changes in poly(A) tail length in the post-transcriptional regulation of gene expression. | 11-03-2011 |
20120028312 | METHODS AND COMPOSITIONS RELATING TO POLYPEPTIDES WITH RNASE III DOMAINS THAT MEDIATE RNA INTERFERENCE - The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target. | 02-02-2012 |
20120122217 | METHODS AND COMPOSITIONS FOR REDUCING TARGET GENE EXPRESSION USING COCKTAILS OF siRNAS OR CONSTRUCTS EXPRESSING siRNAS - The present invention concerns methods and compositions involving the production or generation of siRNA mixtures or pools capable of triggering RNA-mediated interference (RNAi) in a cell. Compositions of the invention include kits that include reagents for producing or generating siRNA pools. The present invention further concerns methods using polypeptides with RNase III activity for generating siRNA mixtures or pools that effect RNAi, including the generation of a number of RNA molecules to the same target gene. | 05-17-2012 |
20140099715 | HIGH POTENCY SIRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES - The present invention provides improved methods of attenuating gene expression through the phenomenon of RNA interference. The invention provides methods of synthesis of double stranded RNAs (dsRNAs) of increased potency for use as small interfering RNA (siRNA). Surprisingly and unexpectedly, siRNAs made by the methods of the invention are significantly more potent than previously available siRNAs. | 04-10-2014 |