| Patent application number | Description | Published |
| 20090162919 | METHODS FOR CONCENTRATING MICROALGAE - The present invention provides commercially viable, large-scale methods for concentrating microalgae with an average diameter of about 20 μm or less. The methods find use in concentrating microalgae with an average diameter of about 5 μm or less, for example, | 06-25-2009 |
| 20090325270 | USE OF 2-HYDROXY-5-OXOPROLINE IN CONJUNCTION WITH ALGAE - Provided herein are exemplary methods for the use of 2-hydroxy-5-oxoproline in conjunction with algae. One exemplary method includes applying an effective amount of 2-hydroxy-5-oxoproline to algae in an aqueous environment to accelerate creation of a high-cell density of the algae. The effective amount of the 2-hydroxy-5-oxoproline may be approximately 0.1 grams per liter of the aqueous environment, or up to approximately 0.1 grams per liter of the aqueous environment. The effective amount of the 2-hydroxy-5-oxoproline may be applied to the aqueous environment at or near a same time, or applied to the aqueous environment over a period of time. Exemplary algae cultivation systems are also provided herein. One exemplary system includes an aqueous environment having a pale-green mutant | 12-31-2009 |
| 20100261922 | Systems and Methods for Extracting Lipids from and Dehydrating Wet Algal Biomass - Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass. | 10-14-2010 |
| 20100317088 | Systems and Methods for Extracting Lipids from Wet Algal Biomass - Presented herein are exemplary systems and methods for extracting lipids from a wet algal biomass. An exemplary method comprises lysing a wet algal biomass with an insoluble granular lysing agent to create a lysate, creating a lipid-rich phase in the lysate, and separating the lipid-rich phase from the lysate. An exemplary system comprises a lysing station for creating a lysate from a wet algal biomass and a separation station for creating and separating a lipid-rich phase from the lysate. According to further exemplary systems and methods, ultrasound may be used in place of or in addition to a lysing agent to lyse the wet algal biomass. | 12-16-2010 |
| 20100330658 | SILICEOUS PARTICLES - Various aspects provide for extracting siliceous particles. Siliceous particles may include or be derived from diatoms. Certain embodiments provide for segregating suspensions into two or more segregation products. In some cases, a first product includes siliceous particles, and a second product may include hydrophobic species. Certain aspects provide for extracting non-siliceous biomass (e.g., lipids). | 12-30-2010 |
| 20110041386 | Extraction From Suspensions - A suspension may include an aqueous liquid and suspended particles. The particles may include a nonpolar and/or hydrophobic substance (e.g., a lipid) substantially contained within polar and/or hydrophilic exterior layers. A method for extracting the suspended lipids may include adding a nonpolar solvent to the suspension and disrupting the exterior layers to expose the lipids to the nonpolar solvent. In some cases, particles may also include interior hydrophilic portions (e.g., intracellular water), which may be exposed to the aqueous liquid via disruption of the exteriors. The mixture may be accelerated to segregate the mixture into first and second products. The first product may have a majority of the nonpolar and/or hydrophobic substances. The second product may have a majority of the polar substances. | 02-24-2011 |
| 20110072713 | Processing Lipids - A method for converting lipids to alkyl esters may include receiving a reactant comprising one or more lipids. In some cases, the reactant may include substantial amounts of polar lipids and/or free fatty acids. Some reactants may be derived from photosynthetic organisms, such as algae and/or diatoms. The reactant may be mixed with an alcohol and a catalyst to form a mixture. The mixture may be heated, for example, to a temperature between 50 and 350 degrees Celsius, including between 80 and 220 degrees Celsius. Pressure may be controlled to be between 1 and 200 bar, including between 10 and 100 bar. At least a portion of the reactant may be converted to one or more alkyl esters. A biofuel may include alkyl esters made from lipids according to various methods. | 03-31-2011 |