Patent application number | Description | Published |
20080254523 | Methods for the Production of Products in Host Cells - The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided. | 10-16-2008 |
20080293124 | Multimeric Oxidoreductases - The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof. | 11-27-2008 |
20080299614 | Metabolically Engineered Bacterial Strains Having Non-Functional Endogenous Gluconate Transporters - The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes. | 12-04-2008 |
20090142843 | Glucose Transport Mutants For Production Of Biomaterial - A method is disclosed for restoring a Glu | 06-04-2009 |
20090156430 | PROMOTER AND PLASMID SYSTEM FOR GENETIC ENGINEERING - This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought. | 06-18-2009 |
20090203102 | COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE - The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. | 08-13-2009 |
20100257778 | PRODUCTION OF FATTY ACID DERIVATIVES - Methods and compositions for producing fatty acid derivatives, for example, fatty esters, are described. | 10-14-2010 |
20100274033 | PRODUCTION OF FATTY ACID ESTERS - Methods of producing fatty acid esters, such as fatty acid ethyl esters, from genetically engineered microorganisms are described. | 10-28-2010 |
20100285567 | TAT SIGNAL PEPTIDES FOR PRODUCING PROTEINS IN PROKARYOTES - This invention provides polynucleotides encoding TAT fusion proteins, and methods for producing proteins of interest in a host cell. In particular, the present invention relates to polynucleotides, vectors, polypeptides and methods for expressing organophosphate-degrading enzymes e.g. organophosphorus hydrolase (OPH) in host cell, such as a | 11-11-2010 |
20110000125 | PRODUCTION OF FATTY ALCOHOLS WITH FATTY ALCOHOL FORMING ACYL-COA REDUCTASES (FAR) - The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as | 01-06-2011 |
20110003353 | Multimeric Oxidoreductases - The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof. | 01-06-2011 |
20110046014 | PROMOTER AND PLASMID SYSTEM FOR GENETIC ENGINEERING - This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought. | 02-24-2011 |
20110237769 | Polymers of isoprene from renewable resources - It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. In some cases, these cells are cultured in a culture medium that includes a carbon source, such as, but not limited to, a carbohydrate, glycerol, glycerine, dihydroxyacetone, one-carbon source, oil, animal fat, animal oil, fatty acid, lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, renewable carbon source, polypeptide (e.g., a microbial or plant protein or peptide), yeast extract, component from a yeast extract, or any combination of two or more of the foregoing. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. It is anticipated that there will be a significant demand for synthetic rubber and other isoprene containing polymers that are synthesized using isoprene of this type which is made from renewable, non-petrochemical based resources. In fact, it is believed that industrial customers and consumers would prefer to purchase isoprene containing polymers that are derived from such environmentally friendly sources to those that are made with isoprene derived from a petrochemical process. It is further believed that customers would be willing to pay premium prices for such environmentally friendly products that are made with renewable resources. However, it is important to be able to verify that such isoprene containing polymers are actually made from non-petrochemical based resources. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ | 09-29-2011 |
20120009635 | Metabolically Engineered Bacterial Strains Having Non-Functional Endogenous Gluconate Transporters - The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes. | 01-12-2012 |
20120064586 | Multimeric Oxidoreductases - The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof. | 03-15-2012 |
20120088271 | Use of an Endogenous 2-Micron Yeast Plasmid for Gene Over Expression - Methods and compositions for making stable recombinant yeast 2 μm plasmids are provided. Homologous recombination is performed to clone a nucleic acid of interest into the yeast 2 μm plasmid. Heterologous nucleic acid subsequences are recombined between an FLP and a REP2 gene of the plasmid. | 04-12-2012 |
20120288907 | Glucose Transport Mutants for Production of Biomaterial - A method is disclosed for restoring a Glu | 11-15-2012 |
20130071908 | COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE - The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. | 03-21-2013 |
20130078699 | COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE - The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. | 03-28-2013 |
20130203135 | MULTIMERIC OXIDOREDUCTASES - The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof. | 08-08-2013 |
20130253141 | POLYMERS OF ISROPENE FROM RENEWABLE RESOURCES - It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ | 09-26-2013 |
20140170718 | MULTIMERIC OXIDOREDUCTASES - The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof. | 06-19-2014 |
20140336423 | PRODUCTION OF SATURATED FATTY ALCOHOLS FROM ENGINEERED MICROORGANISMS - Recombinant bacterial microorganisms are provided which comprise heterologous fatty acyl reductases (“FAR”) polypeptides wherein said microorganisms have been engineered to produce increased amounts of saturated fatty alcohols and methods of making saturated fatty alcohols using the recombinant bacterial microorganisms. | 11-13-2014 |
20140342414 | DIRECT BIOCATALYTIC PRODUCTION OF ACRYLIC ACID AND OTHER CARBOXYLIC ACID COMPOUNDS - The present disclosure relates to biocatalytic methods or processes for the synthesis of acrylic acid and its derivatives, or other carboxylic acid compounds of the formula R—CO2H, wherein R is a carbon chain of 5 carbons or fewer, such as methacrylic acid or 3-hydroxypropionic acid. More specifically, the disclosure relates to methods of using an acyl-CoA hydrolase (such as a thioesterase) as a biocatalyst for the hydrolysis (and removal of the CoA moiety) of a substrate acyl-CoA compound to produce the corresponding carboxylic acid compound, such as acrylic acid. In some embodiments, the disclosure provides non-naturally occurring microorganisms that have been transformed with a heterologous acyl-CoA hydrolase, such as a thioesterase, that is capable of hydrolyzing an acyl-CoA produced in a pathway of the microorganism and produce the corresponding carboxylic acid compound, thereby allowing methods for the direct fermentative production of the compound. | 11-20-2014 |