Essen

Lars-Oliver Essen, Marburg DE

Patent application number	Description	Published
20100184189	PROTEIN FOR THE CHEMOENZYMATIC PRODUCTION OF L-THREO-HYDROXYASPARTATE - A simple mutant of the natural asparagine oxygenase comprises at least the amino acids 13 to 318 of the natural asparagine oxygenase AsnO. In this protein according to the present invention, comprising at least the amino acids 13-318 of AsnO D241N, the amino acid residue at position 241 of the natural asparagine oxygenase AsnO is exchanged from aspartate (D) to asparagine (N). The protein according to the present invention, comprising at least the amino acids 13-318 of AsnO D241N, is produced by means of a directed mutagenesis from the AsnO wild type, cloning of this expression plasmid into a vector, transformation of the vector plasmid construction into a host organism and expression of the recombinant protein. The protein according to the present invention is suitable for the chemoenzymatic and enantioselective production of L-threo-hydroxyaspartate from L-aspartate. The protein is substrate-specific and converts quantitatively L-aspartate into L-threo-hydroxyaspartate.	07-22-2010

Patent application number	Description	Published
20090065013	MOIST SNUFF NON-TOBACCO COMPOSITION AND A METHOD FOR PRODUCING THEREOF - A method for the production of a moist snuff non-tobacco composition, and a composition obtainable from the method are disclosed.	03-12-2009
20100187143	MOIST NON-TOBACCO SNUFF PRODUCT - A moist non-tobacco snuff product in pouch, particulate or compressed form for oral placement is provided. The snuff product includes cocoa fibers in an amount of 5-100% by weight of bulk material of the product, and optionally additional plant fibers. The snuff product may contain flavour supplements of food grade, such as fruits, berries, flowers, herbs, oil of fruits and edible plants. Moreover, there is described a process for preparing moist non-tobacco snuff including cocoa fibres. Cocoa fibers are provided, and optionally additional type(s) of plant fiber(s). The fiber bulk material is optionally grinded or cut and/or sieved, whereupon water, one or more humectants, and NaC1 are added. The mixture is heated, kept heated and finally cooled down. Optionally, additional ingredients chosen from the group consisting of salts, humectants, dyes, and/or flavours are added to provide the moist, non-tobacco snuff product.	07-29-2010

Patent application number	Description	Published
20120031415	NON-TOBACCO MOIST SNUFF COMPOSITION AND A METHOD FOR ITS MANUFACTURE - A non-tobacco snuff composition for oral use including at least one type of plant fibers with a high dietary fiber content, wherein the fiber dietary content in the composition is 15-90 w/w % based on the dry weight of the composition and a method for manufacturing the non-tobacco snuff composition.	02-09-2012