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Ehrenfeld Stolzenbach

Katia Ehrenfeld Stolzenbach, Las Condes, Santiago CL

Patent application numberDescriptionPublished
20090054249DNA FRAGMENTS ARRAY FROM BIOMINING MICROORGANISMS AND METHOD FOR DETECTION OF THEM - The present invention discloses an array of DNA fragments from biomining microorganisms and a method to identify readily and simultaneously said microorganisms in a sample. This method is a useful tool in biomining, in every circumstance where a global understanding of the present microbiological diversity is required, or simply to assess the presence of some microorganism with biomining relevance, either on the mineral, or in a bioleaching heap, in the biomining laboratory or in any other circumstance involving biomining microorganisms. 02-26-2009

Katia Nicole Ehrenfeld Stolzenbach, Las Condes CL

Patent application numberDescriptionPublished
20080207462DNA fragments array from biomining microorganisms and method for detection of them - Microorganisms are used in large-scale heap or tank aeration processes for the commercial extraction of a variety of metals from their ores or concentrates. These include copper, cobalt, gold and, in the past, uranium. The metal solubilization processes are considered to be largely chemical with the microorganisms providing the chemicals and the space (exopolysaccharide layer) where the mineral dissolution reactions occur. Temperatures at which these processes are carried out can vary from ambient to 80° C. and the types of organisms present depends to a large extent on the process temperature used. Irrespective of the operation temperature, biomining microbes have several characteristics in common. One shared characteristic is their ability to produce the ferric iron and sulfuric acid required to degrade the mineral and facilitate metal recovery. Other characteristics are their ability to grow autotrophically, their acid-tolerance and their inherent metal resistance or ability to acquire metal resistance. Although the microorganisms that drive the process have the above properties in common, biomining microbes usually occur in consortia in which cross-feeding may occur such that a combination of microbes including some with heterotrophic tendencies may contribute to the efficiency of the process. The remarkable adaptability of these organisms is assisted by several of the processes being continuous-flow systems that enable the continual selection of microorganisms that are more efficient at mineral degradation. Adaptability is also assisted by the processes being open and non-sterile thereby permitting new organisms to enter. This openness allows for the possibility of new genes that improve cell fitness to be selected from the horizontal gene pool. Characteristics that biomining microorganisms have in common and examples of their remarkable adaptability are described.08-28-2008
20090105085ARRAY OF NUCLEOTIDIC SEQUENCES FOR THE DETECTION AND IDENTIFICATION OF GENES THAT CODIFY PROTEINS WITH ACTIVITIES RELEVANT IN BIOTECHNOLOGY PRESENT IN A MICROBIOLOGICAL SAMPLE, AND METHOD FOR USING THIS ARRAY - The present invention makes known an array of nucleotididc sequences for rapidly and simultaneously identifying the presence of certain genes that codify proteins with activities relevant in biotechnology, present in a microbiological sample, and the method for using this array in the identification of the said genes. Specifically, genes that codify for proteins relevant in Biofilm formation, in CO04-23-2009
20110136125METHOD FOR THE IDENTIFICATION AND QUANTIFICATION OF MICROORGANISMS USEFUL IN BIOMINING PROCESSES - The present invention discloses a method to identify and quantify environmental microorganisms useful in biomining processes. These microorganisms are basically 10, belonging to Bacteria: 06-09-2011

Patent applications by Katia Nicole Ehrenfeld Stolzenbach, Las Condes CL

Katia Nicole Ehrenfeld Stolzenbach, Nunoa CL

Patent application numberDescriptionPublished
20090004727METHOD FOR THE SEPARATION OF MICROORGANISMS ADHERED TO A SOLID SAMPLE BY MEANS OF A PHOSPHATE-BASED DAMPER AND SONICATION OF THE SAMPLE - The present invention provides a method for the separation of microorganisms of a microbiological community present in a solid sample. And once the microorganisms are isolated, they are used in microbiological techniques, or their nucleic acids, both DNA and RNA, are extracted from them for carrying out molecular biology techniques. In accordance with the present invention, the method for the separation of microorganisms adhered to a solid sample comprises the following stages: Providing a solid sample with microorganisms adhered to it; adding to the solid sample a high molarity phosphate-based buffer containing a phosphate solution of a concentration greater than 0.5 M, ethanol, and a non-ionic surfactant; sonicating, and recovering the supernatant, comprising microorganisms separated from the solid sample.01-01-2009