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Doroshenko

Ekaterina Olegovna Doroshenko US

Patent application numberDescriptionPublished
20090238796Preparation for treatment of non-infectious inflammatory intestinal diseases - The invention relates to medicine and can be used for treating non-infectious inflammatory intestinal diseases to enhance efficiency of the non-infectious inflammatory intestinal disease treatment and reduce the length, the inventive preparation contains 5-aminosalicylic acid (5 ASA) or a substance, which decomposes in the organism that 5 ASA is formed, and a liophylically dried microbial mass of live bifido- or lactobacteria or the mixture, said bacteria can be immobilised on a sorbent. The 5 ASA can be powdered or granulated mesalazine or sulphasalazine. To ease the administration of preparation and ensure the dilution of components in defined sectors of the gastrointestinal tract, said components can be grouped in two capsules that jointly represent a single dose, wherein one capsule contains a microbial mass of live bacteria and the other capsule contains mesalazine or sulphasalazine or the capsule containing microbial mass of live bacteria is arranged in the mesalazine or sulphasalazine-containing capsule.09-24-2009

Vera Georgievna Doroshenko, Moscow RU

Patent application numberDescriptionPublished
20090087886METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE - The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus 04-02-2009
20090137010METHOD FOR PRODUCING AMINO ACIDS USING BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY - A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.05-28-2009
20100099153Method for producing L-amino acids using bacteria belonging to the genus escherichia - A method for producing lower alkyl ester of α-L-aspartyl-L-phenylalanine by cultivating a recombinant 04-22-2010
20100184162METHOD FOR PRODUCTION OF AN L-AMINO ACID - A method is provided for producing an L-amino acid by culturing a microorganism belonging to the Enterobacteriaceae family and having the ability to produce an L-amino acid, in a medium to produce and accumulate the L-amino acid in the medium. The microorganism has been modified by introduction of a DNA fragment which includes a pho regulon promoter and a structural gene encoding an L-amino acid biosynthetic enzyme, which is ligated downstream of the promoter so that the gene is expressed by the promoter, and so that the activity of the L-amino acid biosynthetic enzyme is increased by the expression of the gene by the promoter. In this way, the L-amino acid that is produced in the medium can be collected. Furthermore, the phosphorus concentration in the medium is such that the expression of the gene by the promoter is induced.07-22-2010
20110143403METHOD FOR PRODUCING L-AMINO ACIDS USING BACTERIA OF THE ENTEROBACTERIACEAE FAMILY - There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.06-16-2011

Patent applications by Vera Georgievna Doroshenko, Moscow RU

Vladimir M. Doroshenko, Sykesville, MD US

Patent application numberDescriptionPublished
20090152458METHOD AND APPARATUS FOR ION FRAGMENTATION IN MASS SPECTROMETRY - A method for fragmentation of analyte ions for mass spectroscopy and a system for mass spectroscopy. The method produces gas-phase analyte ions, produces gas-phase radical species separately from the analyte ions, and mixes the gas-phase analyte ions and the radical species at substantially atmospheric pressure conditions to produce fragment ions prior to introduction into a mass spectrometer. The system includes a gas-phase analyte ion source, a gas-phase radical species source separate from the gas-phase analyte ion source, a mixing region where the gas-phase analyte ions and the radical species are mixed at substantially atmospheric pressure to produce fragment ions of the analyte ions, a mass spectrometer having an entrance where at least a portion of the fragment ions are introduced into a vacuum of the mass spectrometer, and a detector in the mass spectrometer which determines a mass to charge ratio analysis of the fragment ions.06-18-2009
20100288920METHOD AND APPARATUS FOR ION FRAGMENTATION IN MASS SPECTROMETRY - A method for fragmentation of analyte ions for mass spectroscopy and a system for mass spectroscopy. The method produces gas-phase analyte ions, produces gas-phase odd-electron containing species separately from the analyte ions, and mixes the gas-phase analyte ions and the odd-electron containing species at substantially atmospheric pressure conditions to produce fragment ions prior to introduction into a mass spectrometer. The system includes a gas-phase analyte ion source, a gas-phase odd-electron containing species source separate from the gas-phase analyte ion source, a mixing region where the gas-phase analyte ions and the odd-electron containing species are mixed at substantially atmospheric pressure to produce fragment ions of the analyte ions, a mass spectrometer having an entrance where at least a portion of the fragment ions are introduced into a vacuum of the mass spectrometer, and a detector in the mass spectrometer which determines a mass to charge ratio analysis of the fragment ions.11-18-2010

Vladimir M. Doroshenko, Ellicott City, MD US

Patent application numberDescriptionPublished
20090001263ON-PROBE SAMPLE CLEANUP SYSTEM AND METHOD FOR MALDI ANALYSIS - A system and method for cleanup of biological samples from contaminants prior to spectroscopy analysis. The system includes a support configured to hold a sample including a liquid having at least one group of biological molecules with a surface of the support binding the molecules at a surface tension angle to the liquid of less than 180 degrees. The system includes an evaporator configured to evaporate liquid from the support, a solvent applicator configured to apply a solvent for dissolution of the contaminants in the sample, and a solvent removal device configured to remove applied solvent from the sample and thereby at least partially remove the contaminants.01-01-2009

Patent applications by Vladimir M. Doroshenko, Ellicott City, MD US