Patent application number | Description | Published |
20120064630 | EUKARYOTIC HOST CELL COMPRISING AN EXPRESSION ENHANCER - Invention relates to a eukaryotic host cell comprising a recombinant nucleotide sequence encoding an expression enhancer, which is selected from the group consisting of cLC52, RPL33 and cLC61, and its use in a method of producing a protein of interest (POI). | 03-15-2012 |
20130137141 | PRODUCTION CELL LINE - The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line—to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and—to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture. | 05-30-2013 |
20140242636 | REGULATABLE PROMOTER - A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system. | 08-28-2014 |
20140274761 | CONSTITUTIVE PROMOTER - The invention relates to an isolated nucleic acid sequence comprising a promoter, which is a native sequence of | 09-18-2014 |
20140308718 | FERMENTATION PROCESS FOR PRODUCING CHEMICALS - The invention refers to a method of biotransforming a carbohydrate of a raw material into a chemical, by cultivating | 10-16-2014 |
Patent application number | Description | Published |
20080297089 | Method for Operating a Two-Phase Rotary Current Controller - A method is disclosed for operating a two-phase rotary current controller. In order to optimize the operation of a two-phase rotary current controller, magnitudes of a parameter that can be varied in both controllable phases of a two-phase rotary current controller is matched in these phases. In particular, the existing principle of phase-symmetric control with a uniform ignition delay in both controlled outer conductors is abandoned, and a separate ignition delay is stipulated for each of the two controlled outer conductors. | 12-04-2008 |
20100207554 | METHOD FOR SLOWING-DOWN CONTROL OF AN ASYNCHRONOUS MACHINE - A method is disclosed for slowing-down control of an asynchronous machine, wherein the value of a start energy is determined and stored while the asynchronous machine is being run up from being stationary to the operating rotation speed. The asynchronous machine is braked, in the reversing mode, with braking energy which corresponds to the start energy multiplied by a correction factor wherein the correction factor assumes a value between 0 and 1. In at least one embodiment, the asynchronous machine is braked further by DC braking after braking has been carried out in the reversing mode. | 08-19-2010 |
20110225442 | METHOD FOR THE OPERATION OF SYNCHRONOUS MOTORS, AND ASSOCIATED DEVICE - Synchronous motors are controlled using a three-phase AC power controller. According to at least one embodiment of the invention, a fundamental wave having the desired frequency is defined for the first phase, and corresponding fundamental waves which have the same frequency as the frequency in the first phase but are out of phase by specific values are defined in the other phases. The triggering times during which the generated current pulse concurrently has the same polarity as the respective fundamental wave in two of the phases are determined for each phase and are used. Two of the three fundamental waves are generated in phase opposition when the desired frequency amounts to half the system frequency. | 09-15-2011 |