| Patent application number | Description | Published |
|---|
| 20080213809 | STABLE NAD/NADH DERIVATIVES - The present invention provides for stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives of formula (I), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits. | 09-04-2008 |
| 20080305469 | Fluorimetric Determination of Analytes By An Intramolecular Quencher-Fluorophore Conjugate - A method and reagent for detecting the presence or amount of an analyte by a redox reaction and a fluorimetric determination, is disclosed. The reagent comprises a compound of the general formula Q-F as a redox indicator, wherein Q is a quencher group and F is a fluorophore group. The quencher group Q or/and the fluorophore group F can be reduced or oxidized, and the fluorescence can change depending on the reduction or oxidation. | 12-11-2008 |
| 20090005550 | POLYNUCLEOTIDE CONTAINING A PHOSPHATE MIMETIC - The present invention concerns modified oligonucleotides and processes for their production wherein these oligonucleotides contain at least once the structure P═N-Acc where Acc is an electron acceptor or an electron acceptor substituted with a residue R and R is any organic substituent. | 01-01-2009 |
| 20090012279 | POLYNUCLEOTIDE LABELING REAGENT - The present invention provides a new labeling reagent for preparing modified oligonucleotides and processes for their production wherein these oligonucleotides contain at least once the structure P═N—SO | 01-08-2009 |
| 20090181401 | Detection format for hot start real time polymerase chain reaction - The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles. | 07-16-2009 |
| 20090269766 | NUCLEIC ACID AMPLIFICATION IN THE PRESENCE OF MODIFIED RANDOMERS - The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR. | 10-29-2009 |
| 20090275037 | FLUORESCENT DOUBLE STRANDED DNA BINDING DYES - The present invention is directed to a fluorescent dye comprising a benzothiazolium moiety and a pyrimidinium moiety connected by a mono-methine bridge, characterized in that (i) the 2-position of the pyrimidine carries a substituent which starts with a C-atom and (ii) the 5- and 6-positions of the pyrimidine ring are an integral part of a further aromatic ring structure. | 11-05-2009 |
| 20090306360 | 2-AZAPURINE COMPOUNDS AND THEIR USE - Within oligonucleotides, 2-azapurine and especially 2-azaadenine bases form specifically base pairs with guanine. This base pair is of analogous stability as an adenine-thymine but less stable than a guanine-cytosine base pair. Therefore, the incorporation of 2-azaadenine residues into oligonucleotides instead of cytosine leads specifically to hybridization complexes with nucleic acids with homogenous stability. This is useful for the adaptation of the stabilities of different oligonucleotide sequences in all kinds of hybridization techniques, for example in oligomer chip technology. | 12-10-2009 |
| 20100093990 | Nucleotide with an Alpha-Phosphate Mimetic - Described are modified mononucleotides and processes for their production wherein these nucleotides contain at least once the structure P═N-Acc in which Acc is an electron acceptor or an electron acceptor substituted with a residue R, and R is any organic substituent. | 04-15-2010 |
| 20100248237 | MINIATURIZED, HIGH-THROUGHPUT NUCLEIC ACID ANALYSIS - The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products. | 09-30-2010 |
| 20100248991 | SOLID SUPPORT FOR HIGH-THROUGHPUT NUCLEIC ACID ANALYSIS - The present invention provides a solid support which is preferably a bead comprising at least two sequence specific amplification primers wherein at least one primer is bound to the support with an inducible cleavable linker. The present invention also provides various method for preparing a solid support comprising at least two sequence specific primers, further characterized in that at least one of the primers is cleavable. | 09-30-2010 |
| 20100255547 | POLYANION FOR IMPROVED NUCLEIC ACID AMPLIFICATION - The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3≦m≦6, 30≦n≦60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids. | 10-07-2010 |
| 20100258742 | DYE COMPOSITION FOR LIQUID TRANSFER CONTROL - The present invention provides kits and methods for composition ratio control based on dyes that are designed to enable energy transfer between each other. In more detail, with the method of the present invention it is possible to verify the mixing ratio of a first component comprising a first dye with a second component comprising a second dye. | 10-14-2010 |
| 20100285535 | PCR HOT START BY MAGNESIUM SEQUESTRATION - The present invention is directed to a synthetic peptide having a length of not more than 30 amino acids comprising a divalent cation binding site. Such a peptide according to the present invention is part of a composition for nucleic acid amplification and provides for a so-called hot start effect. | 11-11-2010 |
| 20110092689 | FLUORESCENCE QUENCHER MOLECULES - Disclosed are pyridinyl-isoquinoline-dione derivatives, methods of producing these derivatives, conjugates comprising the pyridinyl-isoquinoline dione derivatives and (i) a solid support, or (ii) a biomolecule, methods of producing these conjugates as well as the use of these conjugates as quenchers in fluorescence resonance energy transfer (FRET). | 04-21-2011 |
| 20110143416 | STABILIZATION OF DEHYDROGENASES WITH STABLE COENZYMES - The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes. | 06-16-2011 |
| 20110151444 | METHOD FOR DETECTION OF AN RNA MOLECULE, A KIT AND USE RELATED THEREFOR - Described is a method for the detection of a RNA molecule, the method involving the steps of providing a sample containing the RNA molecule; hybridizing to the RNA molecule a first polynucleotide; extending the first polynucleotide to generate a first strand cDNA; hybridizing a second polynucleotide to the first strand cDNA; extending the first strand cDNA to generate an extension reaction product; amplifying the extension reaction product by means of polymerase chain reaction; and detecting the amplification product by means of real-time fluorescence readout. Also described is a kit containing a first and a second polynucleotide as defined in the present invention, a set of dNTPs, a reverse transcriptase enzyme, and a detection moiety. | 06-23-2011 |
| 20110151550 | System For Multi Color Real Time PCR - The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm. | 06-23-2011 |
