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De Both

Greta De Both, Wetteren BE

Patent application numberDescriptionPublished
20100248232METHODS FOR DETERMINING THE PRESENCE OR ABSENCE OF ELITE EVENT MS-BN1 IN BRASSICA PLANT MATERIAL - This invention relates to transgenic winter oilseed rape (WOSR) plants, plant material and seeds, harboring a specific transformation event. It pertains to winter oilseed rape plants, more particularly to a pair of winter oilseed rape plants, which is particularly suited for the production of hybrid seed. More specifically, one plant is characterized by being male-sterile, due to the presence in its genome of a male sterility gene, while the other is characterized by carrying a fertility-restorer gene, capable of preventing the activity of the male-sterility gene. The invention further provides a method for producing hybrid seed, a process for producing a transgenic WOSR plant oil or plant, and a method to identify a transgenic plant, cell or tissue. A kit for identifying the transgenic plants comparing the elite event of the present invention is also described. The WOSR plants of the invention combine the ability to form hybrid seeds with optimal overall agronomic performance, genetic stability and adaptability to different generic backgrounds.09-30-2010
20110294133METHODS FOR DETERMINING THE PRESENCE OR ABSENCE OF ELITE EVENT RF-BN1 IN BRASSICA PLANT MATERIAL - This invention relates to transgenic winter oilseed rape (WOSR) plants, plant material and seeds, harboring a specific transformation event. It pertains to winter oilseed rape plants, more particularly to a pair of winter oilseed rape plants, which is particularly suited for the production of hybrid seed. More specifically, one plant is characterized by being male-sterile, due to the presence in its genome of a male sterility gene, while the other is characterized by carrying a fertility-restorer gene, capable of preventing the activity of the male-sterility gene. The invention further provides a method for producing hybrid seed, a process for producing a transgenic WOSR plant oil or plant, and a method to identify a transgenic plant, cell or tissue. A kit for identifying the transgenic plants comparing the elite event of the present invention is also described. The WOSR plants of the invention combine the ability to form hybrid seeds with optimal overall agronomic performance, genetic stability and adaptability to different generic backgrounds.12-01-2011

Michel Theodoor Jan De Both, Wageningen NL

Patent application numberDescriptionPublished
20090307805ALTERNATIVE NUCLEOTIDES FOR IMPROVED TARGETED NUCLEOTIDE EXCHANGE - A method and oligonucleotides for targeted nucleotide exchange of a duplex DNA sequence, wherein the donor oligonucleotide contains at least one modified nucleotide having a higher binding affinity compared to naturally occurring A, C, T or G and/or binds stronger to a nucleotide in an opposite position in the first DNA sequence as compared to a naturally occurring nucleotide complementary to the nucleotide in the opposite position in the first DNA sequence.12-10-2009

Michiel Teodoor Jan De Both, Wageningen NL

Patent application numberDescriptionPublished
20110113512TRICHOME SPECIFIC PROMOTERS - Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising an trichome specific promoter and methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.05-12-2011

Michiel Theodoor Jan De Both, Wageningen NL

Patent application numberDescriptionPublished
20090064377METHOD AND MEANS FOR TARGETED NUCLEOTIDE EXCHANGE - The invention pertains to a method for targeted alteration of a duplex acceptor DNA sequence, comprising combining the duplex acceptor DNA sequence with a donor oligonucleotide, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor oligonucleotide comprises a domain that comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, and wherein a section of the donor oligonucleotide is methylated to a higher degree than the second DNA sequence and/or wherein the second DNA is methylated to a lower degree than the corresponding section of the donor oligonucleotide, optionally in the presence of proteins that are capable of targeted nucleotide exchange and to oligonucleotides for use in the method.03-05-2009
20090328248CONSTITUTIVE PLANT PROMOTERS - Strong, constitutive plant promoters are provided, referred herein to as AA12-31-2009
20100055780TARGETED NUCLEOTIDE EXCHANGE WITH PROPYNYL MODIFIED OLIGONUCLEOTIDES - A method and oligonucleotides for targeted nucleotide exchange of a duplex DNA sequence, wherein the donor oligonucleotide contains at least one modified nucleotide which is a propynylated purine and/or pyrimidine having a higher binding affinity compared to naturally occurring A, C, T or G and/or binds stronger to a nucleotide in an opposite position in the first DNA sequence as compared to a naturally occurring nucleotide complementary to the nucleotide in the opposite position in the first DNA sequence.03-04-2010
20100186124 TARGETED NUCLEOTIDE EXCHANGE WITH LNA MODIFIED OLIGONUCLEOTIDES - A method and oligonucleotides for targeted nucleotide exchange of a duplex DNA sequence, wherein the donor oligonucleotide contains at least one modified nucleotide which is a LNA having a higher binding affinity compared to naturally occurring A, C, T or G and/or binds stronger to a nucleotide in an opposite position in the first DNA sequence as compared to a naturally occurring nucleotide complementary to the nucleotide in the opposite position in the first DNA sequence.07-22-2010
20100291684MUTAGENESIS METHOD USING POLYETHYLENE GLYCOL MEDIATED INTRODUCTION OF MUTAGENIC NUCLEOBASES INTO PLANT PROTOPLASTS - Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.11-18-2010
20110307972FARMESENE SYNTHASE - A new farnesene synthase was isolated from tomato. The farnesene synthase shows surprising properties with regard to the end products formed and its gene has, on a nucleotide level, low sequence identity with known farnesene synthase genes from other sources. The invention relates to isolated polynucleotides, polypeptides encoded by said polynucleotides, genetic constructs, vectors, hosts, in particular plants, harbouring such polynucleotides, polypeptides and genetic constructs, and seed derived from such plants.12-15-2011

Patent applications by Michiel Theodoor Jan De Both, Wageningen NL

Michiel Theodoor Jan De Both, Bg Wageningen NL

Patent application numberDescriptionPublished
20120076751PLANT VOLATILES - The present invention provides compounds and compositions for attracting or repelling sap-sucking insects, such as whitefly, as well as methods for using such compositions.03-29-2012