Cornea
Razvan L. Cornea, Woodbury, MN US
Patent application number | Description | Published |
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20120021926 | FLUORESCENCE RESONANCE ENERGY TRANSFER ASSAYS FOR SARCO/ENDOPLASMIC RETICULUM CALCIUM ATPASE AND PHOSPHOLAMBAN - Provided herein are methods for identifying molecules capable of modulating SERCA, the SERCA-PLB complex or the microenvironment of the complex. An exemplary assay provided herein is fluorescence resonance energy transfer (FRET). Also provided herein are FRET assays that are optimized for high-throughput screening (HTS) for identifying small molecules that modulate SERCA or the SERCA-PLB complex. Further provided are kits for carrying out said methods for identifying molecules. | 01-26-2012 |
Razvan L. Cornea, Minneapolis, MN US
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20150160216 | METHODS TO IDENTIFY MODULATORS OF RYR CALCIUM CHANNELS - Provided herein are methods for identifying a compound that modulates a Ryanodine receptor (Ryr). Fluorescence resonance energy transfer between an FKBP bound to an RyR and fluorescent derivatives of RyR binding partners (e.g., calmodulin) or domain-peptide biosensors is used to provide a readout dependent on the RyR functional state. The methods permit measurement of RyR present in a permeabilized cell or in a purified membrane. | 06-11-2015 |
Razvan L. Cornea, Saint Paul, MN US
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20150204847 | HIGH-THROUGHPUT, HIGH-PRECISION METHODS FOR DETECTING PROTEIN STRUCTURAL CHANGES IN LIVING CELLS - Methods for identifying a compound that alters fluorescence resonance energy transfer (FRET) of a protein. The methods include use of a genetically engineered cell that includes a target protein. The target protein includes one or more heterologous domains. In one embodiment, a target protein includes two heterologous domains, and in another embodiment, the target protein includes a heterologous domain and the cell further includes a second protein that includes a heterologous domain. A heterologous domain may include a chromophore or an amino acid to which a fluorescent dye attaches. The fluorescence lifetime of one or more chromophore, one or more fluorescent dye, or the combination thereof, is measured after contacting the cell with a compound A difference between the fluorescence lifetime in the presence of the test compound and the fluorescence lifetime in the absence of the test compound indicates that the test compound alters the FRET of the target protein. | 07-23-2015 |
Toma Cornea, Hyvinkaa FI
Patent application number | Description | Published |
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20150060588 | ROPE STORAGE UNIT, A METHOD FOR INSTALLING ELEVATOR AND A METHOD FOR FABRICATING ROPE STORAGE UNIT - A rope storage unit includes a rope reel, formed by a rope wound in a spiral form and a support body provided with an inner space inside which the rope reel is positioned supported by the support body. The rope is a rod having a straight form when in rest state and elastically bendable away from the straight form, the rope being under substantial bending tension in the spiral form. The support body includes one or more support members delimiting the inner space and surrounding radially the rope reel. The outer rim of the rope reel radially compresses against the one or more support members as an effect of the bending tension. A method is provided for installing an elevator rope implementing the rope storage unit, as well as to a method for fabricating the rope storage unit. | 03-05-2015 |