Patent application number | Description | Published |
20080259422 | SELECTIVE PHOTOCOAGULATION - A method of scanning a laser beam across a set of cells includes during a first interval, scanning a laser beam across a set of cells; and during a second interval, deflecting the laser beam away from the set of cells. The first interval is selected to cause microcavitation in at least a portion of the cells from the set of cells. | 10-23-2008 |
20080281306 | SELECTIVE PHOTOCOAGULATION - A method of scanning a laser beam across a set of cells includes during a first interval, causing a galvanometric scanner to scan a laser beam across a set of cells; and during a second interval, causing the galvanometric scanner to deflect the laser beam away from the set of cells. The first interval is selected to cause microcavitation in at least a portion of the cells from the set of cells. | 11-13-2008 |
20100049041 | IN VIVO FLOW CYTOMETRY SYSTEM AND METHOD - The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest. | 02-25-2010 |
20100233092 | IN-VIVO MONITORING OF CIRCULATING APOPTOTIC CELLS - The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in-vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest. In some embodiments, the circulating cells comprise apoptotic cells whose detection can allow, e.g., non-invasive monitoring of the efficacy of a cancer treatment, such as an anti-tumor or an anti-angiogenic therapy. | 09-16-2010 |
20110044910 | IN VIVO FLOW CYTOMETRY BASED ON CELLULAR AUTOFLUORESCENCE - The present invention generally provides methods and systems for performing in vivo flow cytometry by using blood vessels as flow chambers through which flowing cells can be monitored in a live subject in vivo without the need for withdrawing a blood sample. In some embodiments, one or more blood vessels are illuminated with radiation so as to cause a multi-photon excitation of an exogenous fluorophore that was previously introduced into the subject to label one or more cell types of interest. In some other embodiments, rather than utilizing an exogenous fluorophore, endogenous (intrinsic) cellular fluorescence can be employed for in vivo flow cytometry. The emission of fluorescence radiation from such fluorophores in response to the excitation can be detected and analyzed to obtain information regarding a cell type of interest. | 02-24-2011 |
20110060232 | RETINAL FLOW CYTOMETRY - The present invention provides methods and devices for performing flow cytometry. In one embodiment, blood circulating through one or more retinal blood vessels of a subject is illuminated in-vivo so as to excite a plurality of fluorescent-labeled cells contained in the blood. The fluorescence radiation emitted by the excited cells is then detected and analyzed to count the cells from which fluorescence is detected. | 03-10-2011 |
20120029490 | DOSE DETERMINATION FOR INDUCING MICROCAVITATION IN RETINAL PIGMENT EPITHELIUM (RPE) - Methods and systems for controlling selective targeting of retinal pigment epithelium (RPE) cells within a treatment region of the RPE. The methods include (a) depositing a selected amount of energy on a test region of the RPE; (b) determining an extent to which microcavitation has occurred in the test region; and (c) on the basis of the determination, either depositing the selected amount of energy on the treatment region, or depositing an increased amount of energy on the test region, and repeating steps (b) and (c). | 02-02-2012 |
20120136258 | Retinal Flow Cytometry - The present invention provides methods and devices for performing flow cytometry. In one embodiment, blood circulating through one or more retinal blood vessels of a subject is illuminated in-vivo so as to excite a plurality of fluorescent-labeled cells contained in the blood. The fluorescence radiation emitted by the excited cells is then detected and analyzed to count the cells from which fluorescence is detected. | 05-31-2012 |
20120296320 | OPTICAL DEVICES AND METHODS FOR SELECTIVE AND CONVENTIONAL PHOTOCOAGULATION OF THE RETINAL PIGMENT EPITHELIUM - The present invention provides devices and methods for applying radiation to the retina of a patient. In one embodiment, an apparatus includes a radiation source for generating a radiation beam suitable for absorption by retinal pigment epithelial cells. One or more optical components are included to direct the beam onto the retina. A scanner is optically coupled to the radiation source to control movement of the beam in two dimensions to allow a scan over the retina. A controller applies control signals to the scanner to adjust beam movement to illuminate a plurality of retinal locations in a temporal sequence according to a predefined pattern. The device can be operated in one mode to effect selective targeting of retinal pigment epithelial cells, or in another mode to effect thermal photocoagulation of the retina. | 11-22-2012 |
20140031647 | IN VIVO FLOW CYTOMETRY BASED ON CELLULAR AUTOFLUORESCENCE - The present invention generally provides methods and systems for performing in vivo flow cytometry by using blood vessels as flow chambers through which flowing cells can be monitored in a live subject in vivo without the need for withdrawing a blood sample. In some embodiments, one or more blood vessels are illuminated with radiation so as to cause a multi-photon excitation of an exogenous fluorophore that was previously introduced into the subject to label one or more cell types of interest. In some other embodiments, rather than utilizing an exogenous fluorophore, endogenous (intrinsic) cellular fluorescence can be employed for in vivo flow cytometry. The emission of fluorescence radiation from such fluorophores in response to the excitation can be detected and analyzed to obtain information regarding a cell type of interest. | 01-30-2014 |
20140350394 | SYSTEMS AND METHODS FOR SENSING, ENUMERATING AND IMAGING RARE CELLS WITH DIFFUSE LIGHT - Diffuse fluorescence flow cytometers and methods of using them include a plurality of excitation sources and a plurality of detectors, all circumferentially arranged about a space for accommodating a limb of a subject. Tomographic reconstructions of cells within the limb are made by varying the intensity and direction of excitation and then analyzing the results. | 11-27-2014 |